[1]黄广宇,毛青,邓国宏,等.HIV-1准种变化与人类白细胞抗原限制性细胞毒性T淋巴细胞表位的相关性研究[J].第三军医大学学报,2008,30(09):814-816.
 HUANG Guang-yu,MAO Qing,DENG Guo-hong,et al.Correlation of diversity of HIV-1 quasispecies and their HLA-restricted CTL epitopes[J].J Third Mil Med Univ,2008,30(09):814-816.
点击复制

HIV-1准种变化与人类白细胞抗原限制性细胞毒性T淋巴细胞表位的相关性研究(/HTML )
分享到:

《第三军医大学学报》[ISSN:1000-5404/CN:51-1095/R]

卷:
30卷
期数:
2008年第09期
页码:
814-816
栏目:
论著
出版日期:
2008-05-15

文章信息/Info

Title:
Correlation of diversity of HIV-1 quasispecies and their HLA-restricted CTL epitopes
作者:
黄广宇毛青邓国宏兰林李俊刚蒋黎周吉军王宇明
第三军医大学西南医院全军感染病研究所
Author(s):
HUANG Guang-yu MAO Qing DENG Guo-hong LAN Lin LI Jun-gang JIANG Li ZHOU Ji-jun WANG Yu-ming
Institute of Infectious Diseases, Southwest Hospital, Third Military Medical University
关键词:
HIV-1准种CTL表位
Keywords:
HIV-1 quasispecies human leukemia antigen cytotoxic T lymphocyte epitope
分类号:
R373.9; R392-33; R392.2
文献标志码:
A
摘要:
目的  研究HIV-1准种变化与人类白细胞抗原(human leucocyte antigen,HLA)限制性细胞毒性T淋巴细胞(cytotoxic T lymphocyte,CTL)表位的相关性。  方法  从2对母婴传播HIV-1感染者血清内提取HIV-1核酸,进行逆转录套式PCR(RT-nested PCR)扩增,产物克隆后,挑选阳性克隆行CSGE分析,对优势和劣势准种进行克隆测序分析,对氨基酸序列中可能的CTL表位进行分析。  结果  第1对感染的母子:母亲YJ体内存在的CTL表位在其54 d的女儿WYF体内都存在,而WYF体内存在1株突变准种,其CTL表位在YJ体内不存在;第2对感染的母子:母亲CM和其12个月的儿子ZDB体内HIV-1 env准种无相同的CTL表位。  结论  HIV-1在改变宿主后,随着时间的延长, HIV-1突变准种所导致HLA限制性CTL表位发生改变的概率增大。
Abstract:
Objective  To study the correlation of diversity of HIV-1 quasispecies and HLA-restricted CTL epitopes.   Methods  RNAs were extracted from the plasma samples of 2 pairs of individuals with HIV-1 vertical transmission (mother YJ and her 54-day daughter WYF, mother CM and her 12-month son ZDB) and polymersed by RT-nested PCR. PCR products were T-A cloned. Positive clones were analyzed by conformation sensitive gel electrophoresis (CSGE). The clones of major and minor quasispecies were sequenced. Sequencing results were analyzed to determine CTL epitopes.   Results  For the first pair mother-child, all CTL epitopes in env region of mother YJ’s HIV-1 quasispecies existed in env region of daughter WYF’s HIV-1 quasispecies, but one of CTL epitopes in env region of WYF’s HIV-1 minor quasispecies was not found in env region of mother YJ’s HIV-1 quasispecies; while for  the second pair mother-child, no same CTL epitodes was found in env region of mother CM’s and son ZDB’s HIV-1 quasispecies.   Conclusion  The longer time after vertical transmission is, the more likely HLA restricted-CTL epitopes will change in mutated HIV-1 quasispecies.

相似文献/References:

[1]刘霖,汤影子,王宇明,等.构象敏感凝胶电泳用于检测乙型肝炎病毒P区核苷类似物耐药准种的初步研究[J].第三军医大学学报,2007,29(21):2103.
[2]陈嵩,王宇明.丙型肝炎病毒结构区基因变异的研究[J].第三军医大学学报,2001,23(03):0.[doi:10.16016/j.1000-5404.2001.03.022 ]
 CHEN Song,WANG Yu ming.[J].J Third Mil Med Univ,2001,23(09):0.[doi:10.16016/j.1000-5404.2001.03.022 ]
[3]蔡林,郑钧丰,谭文婷,等.基于第三代测序技术进行世居藏族人群HBV全长准种特征研究[J].第三军医大学学报,2019,41(07):637.
 CAI Lin,ZHENG Junfeng,TAN Wenting,et al.Quasispecies characteristics of hepatitis B virus full genome in Tibetans studied by the third-generation sequencing [J].J Third Mil Med Univ,2019,41(09):637.
[4]黄广宇,王宇明.人类免疫缺陷病毒1型在垂直传播的个体间病毒基因型和准种的差异性的实验研究[J].第三军医大学学报,2005,27(17):1785.

更新日期/Last Update: 2008-05-07