[1]赛燕,田原,李云鹏,等.急性缺氧条件下氰化钠中毒对大鼠脑组织能量代谢的影响[J].陆军军医大学学报(原第三军医大学学报),2008,30(16):1535-1537.
 SAI Yan,TIAN Yuan,LI Yun-peng,et al.Effects of sodium cyanide intoxication on energy metabolism in rat brain tissues under simulated hypoxia[J].J Amry Med Univ (J Third Mil Med Univ),2008,30(16):1535-1537.
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急性缺氧条件下氰化钠中毒对大鼠脑组织能量代谢的影响(/HTML )
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陆军军医大学学报(原第三军医大学学报)[ISSN:1000-5404/CN:51-1095/R]

卷:
30卷
期数:
2008年第16期
页码:
1535-1537
栏目:
论著
出版日期:
2008-08-30

文章信息/Info

Title:
Effects of sodium cyanide intoxication on energy metabolism in rat brain tissues under simulated hypoxia
作者:
赛燕田原李云鹏王涛叶枫董兆君
第三军医大学军事预防医学院防化医学教研室; 兰州军区疾病预防和控制中心
Author(s):
SAI Yan TIAN Yuan LI Yun-peng WANG Tao YE Feng DONG Zhao-jun
Institute of Chemical Defence, Department of Preventive Medicine, Third Military Medical University, Chongqing 400038;Center for Disease Prevention and Control of Lanzhou Military Command, Lanzhou 730020, China
关键词:
高原低氧氰化钠腺苷酸 海马纹状体
Keywords:
high altitude simulated 4 000 m hypoxia sodium cyanide adenine nucleotide hippocampus corpus striatum
分类号:
R322.81;R364.4;R827.172
文献标志码:
A
摘要:
目的  探讨急性高原缺氧条件下氰化钠中毒对大鼠脑组织能量代谢的影响。 方法  雄性SD大鼠,分为平原组和高原组。平原组动物在本地常规实验室内处理。高原组动物放置于模拟4 000 m海拔高度的低压舱内3 d后开始实验。为大鼠腹腔注射氰化钠(NaCN)3.6 mg/kg染毒,于0、0.5、1、2、4、6 h 6个时相点麻醉后断头取脑。以干湿质量法测定脑组织含水量,伊文思蓝法检测脑组织血管通透性,高效液相色谱(high performance liquid chromatography,HPLC)法测定大鼠脑纹状体和海马组织腺苷三磷酸(adenosine triphosphate,ATP)、腺苷二磷酸(adenosine diphosphate,ADP)和腺苷一磷酸(adenosine monophosphate,AMP)含量。 结果  与平原组比较,高原缺氧环境氰化钠中毒大鼠脑组织含水量增加,纹状体和海马组织ATP和ADP含量减少,AMP含量增加。 结论  高原缺氧可导致大鼠脑组织腺苷酸能量代谢障碍,缺氧条件下氰化钠中毒可进一步加重脑组织能量代谢障碍,同时脑水肿也进一步加重。
Abstract:
Objective    To investigate the effect of sodium cyanide (NaCN) intoxication on brain energy metabolism in rats under hypoxia.     Methods    Male SD rats were randomized equally into normal altitude group and high altitude hypoxia group. The animals in the high altitude hypoxia group were pre-exposed in a hypobaric chamber simulating 4 000 m above sea level for 3 d and were injected with 3.6 mg/kg NaCN intraperitoneally. The rats in the normal altitude group were treated in a routine laboratory in Chongqing (308 m above sea level). The rats were sacrificed, then the brains were isolated, striatum and hippocampus were separated on ice at 0, 0.5, 1.0, 2.0, 4.0 and 6.0 h after intoxication. Brain water content was tested by measuring the ratio of dry/wet weight. Evans blue (EB) determination was used to understand the permeability of blood vessels in the brain. HPLC was employed to test the contents of adenosine monophosphate (ATP), adenosine diphosphate (ADP) and adenosine monophosphate (AMP) in hippocampus and corpus striatum of the rat brain.     Results    The brain water was significantly increased in the rats of high altitude hypoxia group by NaCN intoxication, and ATP and ADP contents were significantly decreased in the tissues of hippocampus and corpus striatum either by hypoxia only or by hypoxia and cyanide intoxications, compared with those in the normal altitude group. However, AMP content in the brain tissues was increased by both.     Conclusion    NaCN intoxication under simulated hypoxia can produce energy metabolism turbulence, which may be involved in brain edema formation.

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更新日期/Last Update: 2008-07-30