[1]温磊,孟刚,李懿,等.人骨肉瘤抗失巢凋亡细胞的生物学特性研究[J].第三军医大学学报,2008,30(10):942-945.
 WEN Lei,MENG Gang,LI Yi,et al.Biological characteristics of anoikis-resistant cells from human osteosarcoma cell line hFOB1.19[J].J Third Mil Med Univ,2008,30(10):942-945.
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人骨肉瘤抗失巢凋亡细胞的生物学特性研究(/HTML )
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《第三军医大学学报》[ISSN:1000-5404/CN:51-1095/R]

卷:
30卷
期数:
2008年第10期
页码:
942-945
栏目:
论著
出版日期:
2008-05-30

文章信息/Info

Title:
Biological characteristics of anoikis-resistant cells from human osteosarcoma cell line hFOB1.19
作者:
温磊孟刚李懿郭乔楠
第三军医大学西南医院病理学研究所
Author(s):
WEN Lei MENG Gang LI Yi GUO Qiao-nan
Institute of Pathology, Southwest Hospital, Third Military Medical University
关键词:
骨肉瘤失巢凋亡细胞迁移细胞增殖
Keywords:
osteosarcomaanoikismigrationproliferation
分类号:
R329.25;R730.23;R738.1
文献标志码:
A
摘要:
目的  获得人骨肉瘤抗失巢凋亡细胞,了解其生物学特性。  方法  采用本室建立的人永生化成骨细胞(hFOB1.19)恶性转化细胞株(转化细胞),用抗黏附培养法,模拟脱离基质情况,获得抗失巢凋亡细胞。电子显微镜、荧光显微镜和流式细胞仪观察悬浮培养后不同时相点细胞的形态学改变和凋亡率。MTT法、Transwell小室分别检测细胞增殖情况和细胞的迁移能力。  结果  形态学观察可见到典型的凋亡细胞,流式细胞仪检测细胞悬浮后24、48、72 h的凋亡(PI染色)情况,凋亡率逐渐升高。抗失巢凋亡细胞再次悬浮同时相凋亡率与转化株相比下降;抗失巢凋亡细胞较对照组(转化细胞)的细胞增殖能力增强,迁移能力增强,转化细胞差异有统计学意义(P<0.05)。  结论  经过14 d的悬浮培养处理后,获得的人骨肉瘤抗失巢凋亡细胞不仅抗失巢凋亡能力有所增强,增殖能力和迁移能力也有增强。
Abstract:
Objective  To obtain anoikis-resistant cells from human osteosarcoma cell line hFOB1.19 and investigate its biological characteristics.   Methods  The osteosarcoma cell line we used was malignant transformed immortalized human fetal osteoblastic human cells (hFOB1.19) which was transformed in our lab (called transformant in this paper). We have used anti-adhesion cell culture method to mimic detachment of tumor cells from extracellular matrix (ECM) to obtain anoikis-resistant variants. Transmission electron microscopy and fluorescence microscopy were used to observe the morphological changes, flow cytometry was used to observe the apoptotic rate. The growth characteristic was observed by MTT. The migration characteristics was examined by transwell cell culture system.   Results  Typical apoptotic cells were seen after de-adhesion culture. The apoptotic rate of the transformant at 24, 48 and 72 h showed a gradually increasing tendency. The apoptotic rate of anoikis-resistant cells was lower than that of the transformant at the same time point. Compared with the transformant, the anoikis-resistant variants had stronger ability in proliferation and in migration with statistical significance (P<0.05).   Conclusion  After 14 days’de-adhesion culture, we obtain the anoikis-resistant variant cells of osteosarcoma cells, which have higher ability in proliferation and migration.

参考文献/References:

温磊,孟刚,李懿,等. 人骨肉瘤抗失巢凋亡细胞的生物学特性研究[J]. 第三军医大学学报,2008,30(10):942-945.

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更新日期/Last Update: 2008-05-27