[1]吴玉卓,张玉婷,毛小荣,等.EGF和HGF体外诱导肝硬化患者骨髓间充质干细胞向肝细胞分化的实验研究[J].第三军医大学学报,2008,30(16):1541-1544.
 WU Yu-zhuo,ZHANG Yu-ting,MAO Xiao-rong,et al.Hepatocyte growth factor and epidermal growth factor induce mesenchymal stem cells isolated from patients with hepatocirrhosis into hepatocyte-like cells in vitro[J].J Third Mil Med Univ,2008,30(16):1541-1544.
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EGF和HGF体外诱导肝硬化患者骨髓间充质干细胞向肝细胞分化的实验研究(/HTML )
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《第三军医大学学报》[ISSN:1000-5404/CN:51-1095/R]

卷:
30卷
期数:
2008年第16期
页码:
1541-1544
栏目:
论著
出版日期:
2008-08-30

文章信息/Info

Title:
Hepatocyte growth factor and epidermal growth factor induce mesenchymal stem cells isolated from patients with hepatocirrhosis into hepatocyte-like cells in vitro
作者:
吴玉卓张玉婷毛小荣陈红
兰州大学第一医院感染科
Author(s):
WU Yu-zhuoZHANG Yu-ting MAO Xiao-rongCHEN Hong
Department of Infection, The First Hospital of Lanzhou University, Lanzhou 730000, China
关键词:
肝硬化干细胞 细胞分化 肝样细胞
Keywords:
cirrhosis stem cells cell differentiation hepatocyte-like cells
分类号:
R329.21;R331.22;R575.202
文献标志码:
A
摘要:
目的  探讨表皮细胞生长因子(epidermal growth factor, EGF)和肝细胞生长因子(hepatocyte growth factor, HGF)诱导肝硬化患者骨髓间充质干细胞(MSCs)向肝细胞分化的可行性。 方法  取肝硬化志愿者骨髓,密度梯度离心法联合贴壁筛选法获取MSCs,分别用含有HGF、EGF、HGF+EGF或不加生长因子的培养基进行培养。用流式细胞仪、免疫细胞化学法鉴定细胞表型,酶联免疫吸附法检测培养上清液中Alb水平。 结果 肝硬化患者骨髓MSCs生长良好,表型为CD29阳性,CD34阴性。诱导组细胞可在21~28 d时,形态变为三角形、多角型或类圆形。诱导组细胞7 d检测到AFP的表达,21、28 d检测出CK18阳性,并以时间依赖方式产生Alb。未诱导组在以上时间点未检测到上述指标。 结论  HGF、EGF均能诱导骨髓MSCs分化为肝样细胞,两者在一定程度上具有联合作用。
Abstract:
Objective    To explore the possibility of differentiation of mesenchymal stem cells isolated from patients with hepatocirrhosis into hepatocyte-like cells induced by EGF and HGF and to lay basis for transplanted autologous bone marrow MSCs in treatment of liver disease at terminal stage.     Methods    Bone marrow cells were obtained from volunteers with liver cirrhosis. MSCs were separated by density gradient centrifugation and were cultured through adhere culture. MSCs were cultured in DMEM medium with HGF, EGF, HGF+EGF or no growth factor. The phenotypes of MSCs were identified by flow cytometry, immunohistochemistry, and Albumin levels in culture supernatants were determined by ELISA.     Results    Growth and division of adherent cells obtained from the patients with hepatocirrhosis were good and the phenotypes of MSCs were CD29 positive and CD34 negative. The shape of MSCs changed from long fusiform to polygonal or round on 21th-28th days in grow factor induced groups. Immunocytochemical analysis for CK18 and AFP showed positive staining reaction for AFP on 7th day, for CK18 on 21st and 28th day in grow factor induced groups with MSCs-induced Alb production increasing in a time-dependent manner. No markers of hepatocyte linear cells were detected in no growth factor induction group.     Conclusion    Both HGF and EGF can induce mesenchymal stem cells to differentiate hepatocyte-like cells alone or coordinately.

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更新日期/Last Update: 2008-07-30