[1]蔡志友,晏勇,余昌胤,等.阿托伐他汀抑制脑缺血再灌注大鼠脑组织MMP-2、MMP-9表达[J].第三军医大学学报,2008,30(19):1828-1831.
 CAI Zhi-you,YAN Yong,YU Chang-yin,et al.Atorvastatin downregulates MMP-2, MMP-9 in brain of rats with focal cerebral ischemia-reperfusion[J].J Third Mil Med Univ,2008,30(19):1828-1831.
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《第三军医大学学报》[ISSN:1000-5404/CN:51-1095/R]

卷:
30卷
期数:
2008年第19期
页码:
1828-1831
栏目:
论著
出版日期:
2008-10-10

文章信息/Info

Title:
Atorvastatin downregulates MMP-2, MMP-9  in brain of rats with focal cerebral ischemia-reperfusion
作者:
蔡志友晏勇余昌胤张骏黄良国胡永涛周妮王凤英
重庆医科大学附属第一医院神经内科,重庆市神经病学重点实验室;遵义医学院附属医院神经内科
Author(s):
CAI Zhi-you YAN Yong YU Chang-yin ZHANG Jun HUANG Liang-guo HU Yong-tao ZHOU Ni WANG Feng-ying
Department of Neurology, The First Affiliated Hospital of Chongqing Medical University, Chongqing 400016;Department of Neurology, The Affiliated Hospital of Zunyi Medical College, Zunyi Guizhou 563003, China
关键词:
脑缺血再灌注阿托伐他汀金属基质蛋白酶
Keywords:
cerebral ischemia-reperfusion atorvastatin matrix metalloproteinase
分类号:
R741.02; R743.21; R972.6
文献标志码:
A
摘要:
目的    观察阿托伐他汀对大鼠脑缺血再灌注脑组织MMP-2、MMP-9表达的影响,探讨阿托伐他汀对脑缺血再灌注脑保护作用机制。    方法    将Wistar大鼠随机分组:正常组(N组)、缺血再灌注组(IR组)、假手术组(NS组)、阿托伐他汀对照组(At组)、阿托伐他汀治疗组(AtT组)、阿托伐他汀预处理组(AtP组)。采用免疫组织化学法检测大鼠脑组织MMP-2、MMP-9表达,用图像分析仪测定光密度值,电镜下分析面密度。    结果    AtT、AtP组脑组织MMP-2、MMP-9表达较IR组明显降低(P<0.05),较NS、N、At组明显增高(P<0.01);梗死灶体积AtT、AtP组较IR组明显降低(P<0.05),较NS、N、At组明显增高(P<0.01)。    结论    阿托伐他汀能抑制大鼠脑缺血再灌注后能脑组织MMP-2、MMP-9表达,发挥脑保护作用。
Abstract:
Objective    To study the neuroprotective mechanism of atorvastatin against the injury induced by cerebral ischemia-reperfusion.     Methods    Wistar rats were randomly divided into: normal group (N), ischemia-reperfusion group (IR), sham-operation group (NS), atorvastatin group (At), atorvastatin treatment group (AtT) and atorvastatin preconditioning group (AtP). The rat model of focal cerebral ischemia-reperfusion was established by inserting a piece of nylon thread into the left internal carotid artery. Ischemia of 30 min and reperfusion of 30 min were carried out alternately for 12 h or 24 h respectively. Their expressions of MMP-2 and MMP-9 in brain tissues were measured by immunohistochemical methods and their optic density value was measured by imaging analysis system. The positive cells for MMP-2 and MMP-9 expressions were counted under optic microscope.     Results    The expressions of MMP-2 and MMP-9 in AtP and AtT groups were lower than those in IR group (P<0.05), significantly higher than those in NS, N, At groups (P<0.01); Infarct volume was smaller than that of in IR group (P<0.05), significantly higher than that in NS, N, At groups (P<0.01).     Conclusion    Atorvastatin can downregulate MMP-2 and MMP-9 expressions and restrain infarct volume during focal cerebral ischemia-reperfusion. The treatment with atorvastatin can protect neural function by maintaining the structural integrality of blood brain barrier.

参考文献/References:

蔡志友,晏勇,余昌胤,等. 阿托伐他汀抑制脑缺血再灌注大鼠脑组织MMP-2、MMP-9表达[J]. 第三军医大学学报, 2008, 30(19):1828-1831.

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更新日期/Last Update: 2008-09-26