[1]肖骏,佘强,罗开良,等.别嘌呤醇对心肌梗死后大鼠心肌细胞凋亡的抑制作用[J].第三军医大学学报,2008,30(08):735-738.
 XIAO Jun,SHE Qiang,LUO Kai liang,et al.Allopurinol inhibits cardiomyocyte apoptosis in rats with myocardial infarction[J].J Third Mil Med Univ,2008,30(08):735-738.
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《第三军医大学学报》[ISSN:1000-5404/CN:51-1095/R]

卷:
30卷
期数:
2008年第08期
页码:
735-738
栏目:
论著
出版日期:
2008-04-30

文章信息/Info

Title:
Allopurinol inhibits cardiomyocyte apoptosis in rats with myocardial infarction
作者:
肖骏佘强罗开良黄开顺曹友德
重庆医科大学: 附属第二医院心血管内科,基础研究所,  基础医学院病理学教研室
Author(s):
XIAO Jun1 SHE Qiang1 LUO Kailiang1 HUANG Kaishun2 CAO Youde
Department of Cardiology, The Second Affiliated Hospital;  Department of Basic Institute; Department of Pathology, Chongqing Medical University
关键词:
别嘌呤醇黄嘌呤氧化酶活性氧细胞凋亡心肌梗死
Keywords:
allopurinol xanthine oxidase reactive oxygen species apoptosis myocardial infarction
分类号:
R322.11;R329.25;R977.6
文献标志码:
A
摘要:
目的 探讨黄嘌呤氧化酶(xanthine oxidase, XO)抑制剂别嘌呤醇对心肌梗死大鼠心肌细胞凋亡的影响。 方法 结扎冠脉前降支制作大鼠心肌梗死模型,随机分为假手术组(sham,n=5),心肌梗死组(MI,n=16)和别嘌呤醇组(50 mg·kg-1·d-1n=15),28 d后检测心肌梗死面积,TUNEL法检测心肌细胞凋亡,免疫组化法检测Fas蛋白表达,Western blot检测XO和caspase 3蛋白表达,比色法测定XO和清除活性氧活力。 结果 MI组非梗死区心肌细胞凋亡指数升高,Fas、XO、caspase 3蛋白表达增强,XO活力增加,清除活性氧活力降低(P<0.01);别嘌呤醇组明显缓解了上述指标的变化。 结论 别嘌呤醇能够抑制非梗死区心肌细胞凋亡,其作用机制可能与减少活性氧生成及降低Fas和caspase 3的表达有关。
Abstract:
Objective To explore the effect of xanthine oxidase inhibitor allopurinol on cardiomyocyte apoptosis in rats after myocardial infarction (MI).  Methods MI model was established by the ligation of anterior descending coronary artery. The survivors were randomly divided into 3 groups: sham operation group (n=5), MI group (n=16) and allopurinol group (n=15, receiving allopurinol 50 mg·kg-1· d-1). After 28 days, the infarct size was measured. In noninfarcted zone (NIZ), cardiomyocyte apoptosis was detected by TUNEL; the expression of Fas was detected by immunohistochemistry; the expressions of xanthine oxidase (XO) and caspase 3 were detected by Western blot. In addition, the activities of XO and ·O2-·OH-scavenging in NIZ were detected by colorimetry.  Results Compared with sham operation group, the apoptosis index and expressions of Fas, XO, caspase 3 in NIZ were significantly increased in MI group. The activity of XO was increased but the activities of ·O2- and ·OH-scavenging were decreased (P<0.01). Compared with MI group, the changes of these parameters above were alleviated in allopurinol group. There was no significant difference in infarct size and XO expression between allopurinol and MI groups (P>0.05).  Conclusion Allopurinol could inhibit the cardiomyocyte apoptosis in NIZ in rats. The protective mechanism of allopurinol involves the reduction of reactive oxygen species and depression of the expressions of Fas and caspase 3.

参考文献/References:

肖骏,佘强,罗开良,等. 别嘌呤醇对心肌梗死后大鼠心肌细胞凋亡的抑制作用[J]. 第三军医大学学报,2008,30(8):735-738.

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[1]王真真,刘地川,蔡敏,等.别嘌呤醇对慢性心衰大鼠心功能的改善作用[J].第三军医大学学报,2014,36(10):1021.
 Wang Zhenzhen,Liu Dichuan,Cai Min,et al.Allopurinol improves cardiac function of chronic heart failure rats[J].J Third Mil Med Univ,2014,36(08):1021.
[2]李晓辉,唐汝愚.抗炎药对心肌梗塞兔脂质过氧化的影响与其抑制中性粒细胞功能的关系[J].第三军医大学学报,1991,13(03):0.[doi:10.16016/j.1000-5404.1991.03.043 ]

更新日期/Last Update: 2008-04-28