[1]李强,程湘,孙守勋,等.乳腺癌细胞过表达iASPP对顺铂作用的影响[J].第三军医大学学报,2008,30(15):1438-1440.
 LI Qiang,CHENG Xiang,SUN Shou-xun,et al.Effect of overexpressed iASPP on breast cancer cell line MCF-7 exposed to cisplatin[J].J Third Mil Med Univ,2008,30(15):1438-1440.
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《第三军医大学学报》[ISSN:1000-5404/CN:51-1095/R]

卷:
30卷
期数:
2008年第15期
页码:
1438-1440
栏目:
论著
出版日期:
2008-08-15

文章信息/Info

Title:
Effect of overexpressed iASPP on breast cancer cell line MCF-7 exposed to cisplatin
作者:
李强程湘孙守勋刘莹
第三军医大学新桥医院:检验科,妇产科
Author(s):
LI Qiang CHENG Xiang SUN Shou-xun LIU Ying
Department of Clinical Laboratory, Department of Gynaecology and Obstetrics, Xinqiao Hospital, Third Military Medical University, Chongqing 400037, China
关键词:
iASPP顺铂乳腺癌细胞
Keywords:
iASPP cisplatin breast cancer cell
分类号:
R73-354; R737.9; R979.11
文献标志码:
A
摘要:
目的  构建过表达iASPP的乳腺癌细胞株,分析过表达iASPP对顺铂(cis-dichlorodiamine platinum,DDP)作用下乳腺癌细胞的影响。 方法  采用RT-PCR方法在乳腺组织中扩增出iASPP,转染入MCF-7,G418筛选获得过表达iASPP的乳腺癌细胞株,MTT和流式细胞仪分析过表达iASPP的乳腺癌细胞株在DDP作用下增殖和凋亡情况。 结果  成功构建了过表达iASPP的乳腺癌细胞株,过表达iASPP可明显降低DDP对乳腺癌细胞增殖的抑制作用(P<0.01),并降低细胞凋亡的百分数(P<0.01)。 结论  过表达iASPP可以明显降低乳腺癌细胞MCF-7对DDP作用的敏感性。
Abstract:
Objective    To analyze the effects of overexpressed iASPP on breast cancer cell line MCF-7.     Methods    The iASPP cDNA was obtained from breast carcinoma tissue by RT- PCR. The breast cancer cell line MCF-7 that overexpressed iASPP was established by stable transfection and G418 selection. The proliferation and apoptosis of the MCF-7 cells that overexpressed iASPP after exposure to cisplatin were detected by MTT and flow cytometry.     Results    The MCF-7 cells that overexpressed iASPP were successfully constructed. Overexpressed iASPP could significantly decrease the apoptosis rate of MCF-7 cells exposed to cisplatin and weaken the inhibitory effects of DDP on the proliferation of MCF-7 cells.     Conclusion    Overexpressed iASPP attenuates the sensitivity of human breast cancer cell line MCF-7 to cisplatin.

参考文献/References:

李强,程湘,孙守勋,等.  乳腺癌细胞过表达iASPP对顺铂作用的影响[J].第三军医大学学报,2008,30(15):1438-1440.

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更新日期/Last Update: 2008-07-24