[1]李静,沈宜,杨麟,等.小鼠肝癌细胞(H22)源外泌体的体内抗肝癌作用研究[J].第三军医大学学报,2008,30(19):1836-1838.
 LI Jing,SHEN Yi,YANG Lin,et al.Antitumor effects of exosomes derived from a mouse hepatoma carcinoma cell line H22[J].J Third Mil Med Univ,2008,30(19):1836-1838.
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《第三军医大学学报》[ISSN:1000-5404/CN:51-1095/R]

卷:
30卷
期数:
2008年第19期
页码:
1836-1838
栏目:
论著
出版日期:
2008-10-10

文章信息/Info

Title:
Antitumor effects of exosomes derived from a mouse hepatoma carcinoma cell line H22
作者:
李静沈宜杨麟段红郑维平
重庆医科大学基础医学院病理生理教研室
Author(s):
LI Jing SHEN Yi YANG Lin DUAN Hong ZHENG Wei-ping
Department of Pathophysiology, College of Basic Medicine, Chongqing Medical University, Chongqing 400016, China
关键词:
外泌体肝细胞癌抗肿瘤免疫肿瘤疫苗
Keywords:
exosomes hepatocellular carcinoma antitumor immunity tumor vaccine
分类号:
R392.32; R73-362; R735.7
文献标志码:
A
摘要:
目的    在荷瘤动物模型观察小鼠肝癌细胞(H22)来源的外泌体(exosomes)激发宿主抗肝癌免疫应答的效应。    方法    用自行分离纯化的小鼠肝癌细胞H22源外泌体免疫小鼠,然后给小鼠皮下注射H22肿瘤细胞,观察肿瘤的生长状况。用MTT法检测小鼠脾细胞增殖情况和细胞毒活性;用免疫组织化学法检测肿瘤组织中CD4+、CD8+T淋巴细胞浸润情况。    结果    该外泌体使肿瘤出现时间延迟,肿瘤生长缓慢,小鼠生存率提高。促进脾淋巴细胞增殖并增强其细胞毒活性,促进CD4+和CD8+T细胞活化。    结论    小鼠肝癌细胞(H22)源外泌体能诱导抗肿瘤应答,有效地诱导特异性的杀伤肿瘤细胞活性,有针对亲本细胞的免疫保护作用。
Abstract:
Objective    To investigate the induction of antitumor response and its immune mechanism of H22 cell-derived exosomes.     Methods    Fifteen BALB/c mice were immunized by H22 cell-derived exosomes three times at weekly intervals, then inoculated with the wild-type H22. Another 15 BALB/c mice that were injected with PBS were used as controls. In the 80-day observation period, the tumor growth and survival time were observed. MTT was used to determine lymphocyte proliferation and specific cytotoxicity of mice splenic cells. Immunohistochemical staining of CD4+ and CD8+ was performed for lymphocyte infiltration in tumor tissues.     Results    Immunization with exosomes derived from H22 delayed the tumor growth and promoted the survival rate of the mice bearing the tumor, and also induced lymphocyte proliferation and triggered strong specific cytotoxic response of spleen lymphocytes and activated CD4+ and CD8+T cells.     Conclusion    Vaccination of H22 cell-derived exosomes is effective in inducing the protective immunity. Furthermore, those exosomes have strong antitumor activities.

参考文献/References:

李静,沈宜,杨麟,等. 小鼠肝癌细胞(H22)源外泌体的体内抗肝癌作用研究[J]. 第三军医大学学报, 2008, 30(19):1836-1838.

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更新日期/Last Update: 2008-09-26