[1]李志方,唐军,李露斯,等.C17.2神经干细胞移植Aβ1-40损伤大鼠海马后的分化及对学习记忆的改善[J].第三军医大学学报,2008,30(07):595-599.
 LI Zhi-fang,TANG Jun,LI Lu-si,et al.Differentiation and therapeutic effects of C17.2 neural stem cells after transplanted into hippocampus of Aβ1-40 injuried rats[J].J Third Mil Med Univ,2008,30(07):595-599.
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C17.2神经干细胞移植Aβ1-40损伤大鼠海马后的分化及对学习记忆的改善(/HTML )
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《第三军医大学学报》[ISSN:1000-5404/CN:51-1095/R]

卷:
30卷
期数:
2008年第07期
页码:
595-599
栏目:
论著
出版日期:
2008-04-15

文章信息/Info

Title:
Differentiation and therapeutic effects of C17.2 neural stem cells after transplanted into hippocampus of Aβ1-40 injuried rats
作者:
李志方唐军李露斯杨丽阴金波徐海伟
第三军医大学:西南医院神经内科,基础医学部生理学教研室,重庆市神经科学研究所,新桥医院神经外科
Author(s):
LI Zhi-fang TANG Jun LI Lu-si YANG Li YIN Jin-bo XU Haiwei
Department of Neurology, Southwest Hospital, Department of Physiology, College of Medicine, Department of Neurosurgery, Xinqiao Hospital, Third Military Medical University
关键词:
阿尔茨海默病神经干细胞细胞移植学习记忆
Keywords:
Alzheimer’s disease neural stem cells cell transplantation learning and memory
分类号:
R329.2; R338.64; R749.1
文献标志码:
A
摘要:
目的 探讨永生化神经干细胞株C17.2移植Aβ1-40损伤大鼠海马后的存活、分化以及细胞移植对AD大鼠的治疗作用。 方法 单侧海马齿回注射凝聚态Aβ1-40复制AD大鼠模型;C17.2神经干细胞移植到海马Aβ1-40注射处,免疫荧光观察移植细胞的存活、分化,Morris水迷宫检测干细胞移植对AD大鼠空间学习记忆障碍的改善情况。 结果 C17.2神经干细胞移植到AD大鼠海马后存活良好,DAPI核染显示移植细胞广泛迁移,Cy3荧光显示大部分移植细胞分化为GFAP阳性细胞,并可见一定数量的移植细胞分化为NF200阳性细胞,其中部分发出类似神经元的长突起。行为学检测表明干细胞移植对AD大鼠空间学习记忆能力有明显改善。 结论 永生化神经干细胞移植AD大鼠海马后能有效存活,移植细胞可分化为胶质细胞、神经元并能改善AD大鼠学习记忆功能障碍。
Abstract:
Objective To explore the differentiation and therapeutic effects of immortalized C17.2 neural stem cells in the rat model of Alzheimer’s disease (AD). Method Pre-aggregated Aβ1-40 was microinjected into the dentate gyrus of rat hippocampus to mimic AD, and C17.2 neural stem cells were transplanted into the vicinity of Aβ1-40 2 weeks later. The survival and differentiation of grafted cells were observed with immunofluorescence staining. Learning and memory abilities of animals were evaluated by Morris water maze test. Results The grafted cells survived and migrated extensively. Most C17.2 cells differentiated into GFAP-positive glia and some into NF200-positive neurons with long processes into the host brain. Learning and memory of AD rats were significantly improved after C17.2 neural stem cell transplantation. Conclusion The satisfactory survival, differentiation and improvement on learning and memory indicate the therapeutic potential of immortalized neural stem cells for neuronal replacement.

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更新日期/Last Update: 2008-04-16