[1]王跃红,赵文清,李文玲,等.白介素-24抑制胶质瘤细胞生长的体内外实验研究[J].陆军军医大学学报(原第三军医大学学报),2008,30(03):229-232.
 WANG Yue-hong,ZHAO Wen-qing,LI Wen-ling,et al.Interleukin 24 suppresses the growth of malignant glioma cells in vitro and in vivo[J].J Amry Med Univ (J Third Mil Med Univ),2008,30(03):229-232.
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白介素-24抑制胶质瘤细胞生长的体内外实验研究(/HTML )
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陆军军医大学学报(原第三军医大学学报)[ISSN:1000-5404/CN:51-1095/R]

卷:
30卷
期数:
2008年第03期
页码:
229-232
栏目:
论著
出版日期:
2008-02-15

文章信息/Info

Title:
Interleukin 24 suppresses the growth of malignant glioma cells in vitro and in vivo
作者:
王跃红赵文清李文玲潘宝根梁传栋赵晓云
河北医科大学神经外科教研室;河北省人民医院:功能神经外科,神经外科,临床研究中心
Author(s):
WANG Yue-hong ZHAO Wen-qing LI Wen-ling PAN Bao-gen LIANG Chuan-dong ZHAO Xiao-yun
Department of Neurosurgery, Hebei Medical University; Department of Function Neurosurgery, Department of Neurosurgery, Clinical Research Center, Hebei General Hospital
关键词:
白介素-24基因治疗胶质瘤腺病毒感染
Keywords:
IL-24 gene therapy glioma adenovirus infection
分类号:
R73-362; R730.264; R979.19
文献标志码:
A
摘要:
目的 探讨白介素-24基因转染对胶质瘤生长的影响。  方法  将载有人白介素-24 cDNA的新型重组腺病毒(Ad5F35-IL24)感染人胶质瘤细胞系U251,体外观察Ad5F35IL24对该细胞系生长的影响;建立人胶质瘤动物模型,瘤内注射Ad5F35-IL24,观察肿瘤生长情况;用流式细胞仪检测其凋亡和Bax/Bcl-2的表达情况。  结果  Ad5F35-IL24感染U251细胞和肿瘤组织后,IL-24蛋白高表达,U251细胞和组织的生长受到明显抑制,其凋亡率明显升高,Bax/Bcl-2也明显升高。  结论  白介素-24具有抑制人胶质瘤细胞系U251生长和诱导凋亡作用。Ad5F35-IL24转导白介素-24基因可能是一种有效的胶质瘤基因治疗途径。
Abstract:
Objective To explore the potential use and the effect of adenovirus-mediated interleukin 24 gene (Ad5F35-IL24) therapy for malignant glioma.   Methods  After Ad5F35-IL24 was transfected into human malignant glioma cell line U251, we observed the growth of U251 cells. Ad5F35-IL24 was injected into tumor nodules of BALB/c mouse models of human glioma, and the tumor growth was observed. The apoptosis of tumor cells and their Bax/Bcl-2 expressions were detected with flow cytometer.   Results  The expression of IL-24 proteins were expressed highly after transfection of Ad5F35-IL24. The growth of U251 cells and xenograft tumors was suppressed obviously, and their apoptosis and Bax/Bcl-2 expression were significantly elevated.   Conclusion  IL-24 induces growth suppression and apoptosis in human glioma cells U251. Adenovirus-mediated IL-24 gene therapy is a potential effective approach in the treatment of malignant glioma.

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更新日期/Last Update: 2008-05-04