[1]陈章荣,罗开良,殷跃辉,等.蛋白酶体抑制剂MG-132改善大鼠心肌梗死后心肌结构重塑[J].第三军医大学学报,2008,30(08):750-753.
 CHEN Zhang-rong,LUO Kai-liang,YIN Yue-hui,et al.Proteasome inhibitor MG132 improves left ventricular remodeling after myocardial infarction in rats[J].J Third Mil Med Univ,2008,30(08):750-753.
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《第三军医大学学报》[ISSN:1000-5404/CN:51-1095/R]

卷:
30卷
期数:
2008年第08期
页码:
750-753
栏目:
论著
出版日期:
2008-04-30

文章信息/Info

Title:
Proteasome inhibitor MG132 improves left ventricular remodeling after myocardial infarction in rats
作者:
陈章荣罗开良殷跃辉胡蓉肖骏
重庆医科大学附属第二医院心血管内科
Author(s):
CHEN Zhang-rong LUO Kai-liang YIN Yue-hui HU Rong XIAO Jun
Department of Cardiology, The Second Affiliated Hospital of Chongqing Medical University
关键词:
心肌梗死心肌结构重塑蛋白酶体抑制剂NF-κBIL-1β
Keywords:
myocardial infraction myocardial remodeling proteasome inhibitor NF-κB IL-1β
分类号:
R364.33;R392.3;R542.22
文献标志码:
A
摘要:
目的 探讨MG-132对大鼠心肌梗死(心梗)后心肌结构重塑的影响及机制。 方法 制作大鼠心梗模型,分为MG-132干预(MG)组和心梗对照(MI)组,另设假手术(SH)组,每组大鼠16只。MG组于术后24 h腹腔注射MG-132(0.1 mg·kg-1·d-1),MI组及SH组注射生理盐水对照。连续给药28 d,然后超声检测心脏左室前壁厚度、左室后壁厚度、左室舒张末期直径;处死动物计算左室重量指数及梗死面积,RT-PCR和Western blot分别检测核转录因子-κB p65 (NF-κB p65)及白介素-1β(IL-1β)的mRNA和蛋白质表达量。 结果 梗死面积在MI组和MG组间差异无统计学意义(P>0.05)。与SH组比较,MI组和MG组的左室后壁厚度、左室舒张末直径、左室质量指数明显增大,左室前壁厚度明显减少, NF-κBp65和IL-1β的mRNA及蛋白质表达量明显增加,差异均具有统计学意义(P<0.01)。与MI组比较,MG组左室后壁厚度、左室舒张末直径、左室质量指数,左室前壁厚度改变明显减轻,NF-κB p65和IL-1β的mRNA及蛋白质表达量明显降低,差异具有统计学意义(P<0.01)。 结论 MG-132能一定程度地改善大鼠心梗后心肌结构重塑,其机制可能与抑制NF-κB激活,下调炎性细胞因子如IL-1β表达有关。
Abstract:
Objective To investigate the effects and possible mechanisms of proteasome inhibitor MG-132 on left ventricular remodeling following acute myocardial infarction in rats.  Methods The myocardial infarction model in rats was induced by ligating left anterior descending coronary artery. Thirty-two adult Sprague-Dawley rats that survived 24 h after acute myocardial infarction were randomly divided into myocardial infarction (MI) group and MG-132-treated (MG) group. Sixteen matchable rats were designated as sham-operated group (SH group). Rats in MG group were treated with MG-132 (0.1 mg/kg, i.p. daily) for 28 d, while rats in MI group and SH group were given normal saline as control. On 28th day, the changes of left ventricle structure were measured by echocardiography. The left weight index and infarct size were evaluated. The mRNA and protein levels of NF-κB p65 and IL-1β were determined by reverse transcription-polymerase chain reaction (RT-PCR) and by Western blot, respectively.  Results Compared with SH group, the values of left ventricle posterior wall thickness (LVPWT) and left ventricle end diastolic diameter (LVEDD) were significantly increased in MI group and MG group (P<0.01). However, the values of left ventricle anterior wall thickness (LVAWT) were remarkably decreased (P<0.01). The values of LVPWT, LVEDD and LVW/BW in MG group were notably decreased than those in MI group (P<0.01), whereas the changes of LVAWT in MG group increased  (P<0.01). Moreover, the mRNA and protein levels of NF-κB p65 and IL-1β in MG group were lower than those in MI group (P<0.01).  Conclusion Left ventricular remodeling following acute myocardial infarction could be improved by proteasome inhibitor MG-132 through suppressing NF-κB activation and the expression of inflammatory factor such as IL-1β in rats.

参考文献/References:

陈章荣,罗开良,殷跃辉,等. 蛋白酶体抑制剂MG-132改善大鼠心肌梗死后心肌结构重塑[J]. 第三军医大学学报,2008,30(8):750-753.

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更新日期/Last Update: 2008-04-28