[1]范伟,张莹,伍晓汀.血红素加氧酶-1在大鼠原位肝移植急性排斥反应中的作用[J].陆军军医大学学报(原第三军医大学学报),2008,30(17):1654-1657.
 FAN Wei,ZHANG Ying,WU Xiao-ting.Role of HO-1 in acute rejection after orthotopic liver transplantation in rats[J].J Amry Med Univ (J Third Mil Med Univ),2008,30(17):1654-1657.
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血红素加氧酶-1在大鼠原位肝移植急性排斥反应中的作用(/HTML )
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陆军军医大学学报(原第三军医大学学报)[ISSN:1000-5404/CN:51-1095/R]

卷:
30卷
期数:
2008年第17期
页码:
1654-1657
栏目:
论著
出版日期:
2008-09-15

文章信息/Info

Title:
Role of HO-1 in acute rejection after orthotopic liver transplantation in rats
作者:
范伟 张莹 伍晓汀
贵州省人民医院肝胆外科;    四川大学华西医院普通外科
Author(s):
FAN Wei ZHANG Ying WU Xiao-ting
Department of Hepatobiliary Surgery, Guizhou Provincial Hospital, Guiyang 550002;Department of General Surgery, West China University, Chengdu 610041, China
关键词:
血红素加氧酶-1 原位肝移植 大鼠
Keywords:
hemooxygenase-1 orthotopic liver transplantation rat
分类号:
R345; R392.4; R657.3
文献标志码:
A
摘要:
目的    探讨血红素加氧酶-1(hemooxygenase-1, HO-1)在大鼠原位肝移植急性排斥反应中的作用。    方法    建立大鼠原位肝移植排斥模型后分为3组:对照组(A组),ZnPP组(B组)和CoPP组(C组)。3组分别于3、7 d处死,取血及肝脏标本。进行肝功测定(AST、ALT、TBIL、ALB);肝组织HE染色;荧光RT-PCR检测HO-1表达。    结果    ①3组肝功能3、7 d时比较有明显的统计学意义(P<0.05)。②HE染色:A组3 d出现Ⅰ、Ⅱ级为主的排斥反应,7 d时出现Ⅱ级为主的排斥反应;B组3 d出现Ⅱ级为主的排斥反应,7 d时出现Ⅱ、Ⅲ级为主的排斥反应;C组3 d出现排斥反应0~Ⅱ级,以Ⅰ级为主,7 d时出现排斥反应0~Ⅱ级,以Ⅰ、Ⅱ级为主。③HO-1在C组表达明显升高,B组不明显,A组介于两组之间。    结论    血红素加氧酶-1(HO-1)过表达能减轻急性排斥反应。
Abstract:
Objective    To investigate the effect of HO-1 on acute rejection after orthotopic liver transplantation (OLT) in rats.      Methods    Taking dark agouti (DA) rats as donors and Lewis rats as recipients, OLT was performed. Before OLT, the DA rats and Lewis rats were matched in pairs, and divided into control group, ZnPP group and CoPP group, the paired rats of which intraperitoneally received normal saline, 20 mg/kg ZnPP and 5 mg/kg CoPP respectively 24 h before OLT. Ten pairs of rats were in each group. On day 3 or 7 after OLT, recipient rats were killed, then blood sample and liver specimen were collected. Plasm levels of aspartate aminotransferase (AST), alanine aminotransferase (ALT), total bilirubin (TBIL), albumin (ALB) were measured; HE staining and MPO staining were performed; The expression of HO-1 was detected by the fluorence real time RT-PCR.     Results    On day 3 and 7 after OLT, AST, ALT and TBIL showed significant statistical significance among the three groups; ALB was highest in CoPP group, then in control group, lowest in ZnPP group. On day 3 after OLT, control group had acute rejection of degree one to degree two, ZnPP group had acute rejection of degree two; CoPP group mainly had acute rejection of degree one; On day 7 after OLT, control group mainly had acute rejection of degree two; ZnPP group had acute rejection of degree two to degree three; CoPP group had acute rejection of degree one to two. The expression of HO-1 was highest in CoPP group, then control group, and lowest in ZnPP group.     Conclusion    The expression of HO-1 in OLT with acute rejection in rats can palliate the acute rejection.

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更新日期/Last Update: 2008-09-16