[1]魏勇,顾健腾,鲁开智,等.瑞芬太尼对肝细胞缺氧复氧损伤保护作用的机制研究[J].第三军医大学学报,2007,29(20):1976-1978.
　WEI Yong,GU Jian-teng,LU Kai-zhi,et al.Remifentanyl protects hepatocytes against anoxia-reoxygenation injury[J].J Third Mil Med Univ,2007,29(20):1976-1978.
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瑞芬太尼对肝细胞缺氧复氧损伤保护作用的机制研究(/HTML )
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《第三军医大学学报》[ISSN:1000-5404/CN:51-1095/R]

卷:
29卷
期数:
2007年第20期
页码:
1976-1978
栏目:
论著
出版日期:
2007-10-30

文章信息/Info

Title:
Remifentanyl protects hepatocytes against anoxia-reoxygenation injury
作者:
魏勇; 顾健腾; 鲁开智; 陶国才
第三军医大学西南医院麻醉科
Author(s):
WEI Yong;  GU Jian-teng;  LU Kai-zhi;  TAO Guo-cai
Department of Anesthesiology, Southwest Hospital, Third Military Medical University, Chongqing 400038, China
关键词:
瑞芬太尼; 肝细胞; 缺氧; 复氧; 蛋白激酶C
Keywords:
remifetanil;  hepatocytes;  anoxia;  reoxygenation;  protein kinase C
分类号:
R322.47;R364.4;R791.1
文献标志码:
A
摘要:
目的  探讨瑞芬太尼对缺氧复氧的人肝细胞损伤的作用及其可能的作用机制。 方法  将培养的人肝细胞分为5组，分别为正常对照（C）组、单纯缺氧复氧损伤（AR）组、瑞芬太尼（R）组、白屈菜红碱（CH）组和混合（R+CH）组，n=6。C组正常培养，其余各组缺氧培养15 h，复氧5 h，然后收集细胞，进行：①肝细胞线粒体的丙二醛（MDA）含量测定；②用Annexin V/PI染色，于流式细胞仪下计数凋亡细胞的百分数；③提取RNA，以PKC mRNA为目的基因，进行RTPCR，然后电泳，对条带的吸光值进行比较。 结果  AR组的MDA含量明显高于C组，R组和CH组的MDA含量介于AR与C组之间（P<0.05）。AR组的肝细胞凋亡率明显高于C组，R组和CH组的肝细胞凋亡率介于AR与C组之间（P<0.05）。AR组的PKC mRNA转录量明显高于C组，R组和CH组的PKC mRNA转录量介于AR与C组之间（P<0.05），R+CH组的PKC mRNA转录量低于C组（P<0.05）。 结论  瑞芬太尼对缺氧复氧的肝细胞具有保护作用，且可能与其抑制PKC mRNA的转录有关。
Abstract:
Objective    To assess the protective effect of remifentanil on cultured human hepatocytes against anoxia-reoxygenation injury.     Methods    Cultured hepatocytes were divided into 5 groups: group C receiving normoxia as control; groups AR, R, CH, R+CH receiving 15-hour xypoxia followed by 5-hour reoxygenation (group R receiving 5 ng/ml remifentanil, group CH 10 μmol/L chelerythrine, group R+CH 5 ng/ml remifentanil and 10 μmol/L chelerythrine before reoxygenation). The content of MDA in the hepatocyte mitochondria were measured. The rate of apoptotic cells was measured by flow cytometry. The expression of protein kinase C mRNA was measured by RT-PCR.     Results    Anoxia-reoxygenation caused dramatic increase in the content of MDA, the rate of apoptotic cells and the expression of protein kinase C mRNA. The three indexes mentioned above of groups R and CH were between that of groups C and AR (P<0.05). The expression of protein kinase C mRNA in group R+CH was less than that in group C (P<0.05).     Conclusion    Remifentanil can protect cultured hepatocytes against anoxia-reoxygenation injury, and its mechanism may be associated with protein kinase C mRNA.

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更新日期/Last Update: 2008-07-11