[1]金旭红,杨柳,段小军,等.体外纳米磁标记骨髓间充质干细胞生物学特性及其MR成像[J].第三军医大学学报,2008,30(04):275-279.
 JIN Xu-hong,YANG Liu,DUAN Xiao-jun,et al.Biological characteristics and in vitro MRI of superparamagnetic ironoxide labeled bonederived mesenchymal stem cells from rabbits[J].J Third Mil Med Univ,2008,30(04):275-279.
点击复制

体外纳米磁标记骨髓间充质干细胞生物学特性及其MR成像(/HTML )
分享到:

《第三军医大学学报》[ISSN:1000-5404/CN:51-1095/R]

卷:
30卷
期数:
2008年第04期
页码:
275-279
栏目:
论著
出版日期:
2008-02-28

文章信息/Info

Title:
Biological characteristics and in vitro MRI of superparamagnetic ironoxide labeled bonederived mesenchymal stem cells from rabbits
作者:
金旭红杨柳段小军陈伟李忠
(第三军医大学西南医院: 关节外科中心, 放射科
Author(s):
JIN Xu-hong YANG Liu DUAN Xiao-jun CHEN Wei LI Zhong
Center of Joint Surgery, Department of Radiology, Southwest Hospital, Third Military Medical University
关键词:
超顺磁性氧化铁粒子骨髓间充质干细胞磁标记细胞磁共振成像
Keywords:
superparamagnetic iron oxide particles bone-derived mesenchymal stem cells magnetically labeled cell magnetic resonance imaging
分类号:
R329.1;R445.2;R811.5
文献标志码:
A
摘要:
目的 研究超顺磁性氧化铁粒子(superparamagnetic iron oxide particles, SPIO)体外标记兔骨髓间充质干细胞(mesenchymal stem cells, MSCs)及MR细胞成像示踪可行性。  方法 从兔骨髓中分离培养MSCs,体外不同浓度SPIO联合硫酸鱼精蛋白标记,未标记细胞设为对照组。普鲁士蓝染色和电镜检查鉴定细胞内铁颗粒,台盼蓝染色检测细胞存活,MTT法测定细胞生长曲线的变化,磁标记MSCs转入成骨、成脂肪培养基中进行诱导培养后进行鉴定,应用1.5T MR梯度回波T2加权(GRE T2*WI)扫描序列和自旋回波T2加权(SE T2WI)扫描序列对磁标记细胞成像示踪。  结果 普鲁士蓝染色和电镜检查显示细胞质内含致密铁颗粒,磁标记对MSCs活性和增殖无统计学差异(P>0.05),标记细胞可正常成骨、成脂肪分化。GRE T2*WI序列和SE T2WI序列提示与未标记细胞信号强度(SI)相比,1×106(标记细胞)、5×105(标记细胞)SI均显著性下降(P<0.05),其中GRE T2*WI的信号强度衰减率(△SI)显著高于T2WI序列(P<0.05)。在2个序列中1×106(标记细胞)△SI均高于5×105(标记细胞)△SI,但不具有显著性差异(P>0.05)。  结论 SPIO联合硫酸鱼精蛋白转染剂能成功标记MSCs,磁标记对细胞存活、增殖及潜在多向分化能力无影响。磁标记细胞在MR上产生特征性的低信号改变。应用1.5T MR成像示踪标记细胞可行,以GRE T2*WI序列成像最为敏感。
Abstract:
Objective To label rabbit bone marrow-derived mesenchymal stem cells (MSCs) with superparamagnetic iron oxide particles (SPIO) and study the effects of magnetic labeling on the proliferation and differentiation of MSCs.  Methods Rabbit MSCs were isolated, purified, expanded, then incubated with various doses of SPIO complexed to protamine sulfate (Pro) transfection agents for 12 h. Prussian blue staining and transmission electron microscopy were performed to show intracellular iron. Cell viability and differentiation were evaluated. Vials containing cells underwent 1.5T MR imaging (MRI) with GRE T2*WI weighted and SE T2WI sequence, and the signal intensity were measured and statistically analyzed.  Results Intracytoplasmic nanoparticles were confirmed by Prussian blue staining and transmission electron microscopy. As compared with the unlabeled cells, no significant difference was found in cell viability, growth rate and differentiation of the labeled MSCs. GRE T2*WI and T2*WI demonstrated significant decrease of signal intensity (SI) in vials containing 1×106 and 5×105 labeled cells, in comparison with unlabeled cells (P<0.05). The percentage change of SI (△SI) was significantly higher in 1×106labeled cells than that in 5×105 labeled cells, particularly on GRE T2*WI (P<0.05). Among pulse sequences, GRE T2 demonstrated the highest △SI ut significant (P<0.05).  Conclusion MSCs can be labeled with Fe-Pro efficiently witho change in cell  viability and differentiation capacity. Visualization of SPIO-labeled MSCs by 1.5T MRI is feasible.

参考文献/References:

金旭红, 杨柳, 段小军,等. 体外纳米磁标记骨髓间充质干细胞生物学特性及其MR成像[J].第三军医大学学报,2008,30(4):275-279.

相似文献/References:

[1]李茂华,滕苗.严重烧伤大鼠血清对骨髓间充质干细胞迁移的影响[J].第三军医大学学报,2015,37(16):1643.
 Li Maohua,Teng Miao.Serum from severely burned rats promotes migration in rat bone marrow mesenchymal stem cells through PI3K/Akt signal pathway[J].J Third Mil Med Univ,2015,37(04):1643.
[2]张蕾,陈沅,田杰,等.心肌细胞介导骨髓间充质干细胞的心肌样分化[J].第三军医大学学报,2005,27(16):1681.
[3]王颖楠,范雪梅,赵敏,等.脱细胞膀胱基质复合大鼠骨髓间充质干细胞体外构建组织工程化吊带治疗压力性尿失禁的初步研究[J].第三军医大学学报,2012,34(22):2269.
 Wang Yingnan,Fan Xuemei,Zhao Min,et al.Preliminary study on the construction of a tissue-engineered sling with BMSCs and UBM in vitro for treating stress urinary incontinence[J].J Third Mil Med Univ,2012,34(04):2269.
[4]周长立,任先军,蒋涛,等.Wnt7a基因对大鼠骨髓间充质干细胞增殖及向神经元样细胞分化的影响[J].第三军医大学学报,2013,35(08):702.
 Zhou Changli,Ren Xianjun,Jiang Tao,et al.Wnt7a gene stimulates mesenchymal stem cell proliferation and differentiation into neuron-like cells[J].J Third Mil Med Univ,2013,35(04):702.
[5]郝磊,邹仲敏,王军平,等.hPDGF-A/hBD2双基因共表达腺病毒载体的构建及表达[J].第三军医大学学报,2007,29(10):859.
 HAO Lei,ZOU Zhong-min,WANG Jun-ping,et al.Construction and identification of recombinant adenovirus expressing hPDGF-A and hBD2[J].J Third Mil Med Univ,2007,29(04):859.
[6]郭书权,吴雪晖,许建中,等.两种方法分离小型猪骨髓间充质干细胞的比较[J].第三军医大学学报,2007,29(10):988.
[7]黄文秋,黄宏,徐祥,等.mTOR及其下游信号通路在骨髓间充质干细胞氧化应激损伤中的变化及作用[J].第三军医大学学报,2013,35(02):114.
 Huang Wenqiu,Huang Hong,Xu Xiang,et al.Changes and roles of mTOR and its downstream signaling passway in mouse bone marrow stem cells with oxidative stress injury[J].J Third Mil Med Univ,2013,35(04):114.
[8]冯一梅,徐辉,邹仲敏,等.hPDGF-A/hBD2双基因转染对大鼠骨髓间充质干细胞生物学特性的影响[J].第三军医大学学报,2008,30(06):472.
 FENG Yi-mei,XU Hui,ZOU Zhong-min,et al.Effects of hPDGF-A/hBD2 genes transfection on rat bone marrow mesenchymal stem cells[J].J Third Mil Med Univ,2008,30(04):472.
[9]姚青,宋治远,马显光.脉冲微交流电刺激促进体外诱导大鼠骨髓间充质干细胞向心肌分化[J].第三军医大学学报,2008,30(05):410.
 YAO Qing,SONG Zhi-Yuan,MA Xian-guang.Electrical stimulation promotes the differentiation of rat bone marrow mesenchymal stem cells to cardiomyocyte induced by 5-azacytidine in vitro[J].J Third Mil Med Univ,2008,30(04):410.
[10]朱淑霞,李永华,宋治远,等.电磁场促进骨髓间充质干细胞体外诱导分化时细胞增殖[J].第三军医大学学报,2008,30(05):421.
 ZHU Shu-xia,LI Yong-hua,SONG Zhi-yuan,et al.Pulsed electromagnetic fields improve proliferation of rat marrow mesenchymal stem cells induced by 5-azacytidine[J].J Third Mil Med Univ,2008,30(04):421.

更新日期/Last Update: 2008-04-29