[1]方玉强,黄岚,赵晓辉,等.内皮脂肪酶对人脐静脉内皮细胞ICAM-1表达的影响及量效关系[J].第三军医大学学报,2008,30(17):1615-1617.
 FANG Yu-qiang,HUANG Lan,ZHAO Xiao-hui,et al.Effects of endothelial lipase on ICAM-1 expression in human umbilical vein endothelial cells[J].J Third Mil Med Univ,2008,30(17):1615-1617.
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《第三军医大学学报》[ISSN:1000-5404/CN:51-1095/R]

卷:
30卷
期数:
2008年第17期
页码:
1615-1617
栏目:
论著
出版日期:
2008-09-15

文章信息/Info

Title:
Effects of endothelial lipase on ICAM-1 expression in human umbilical vein endothelial cells
作者:
方玉强黄岚赵晓辉尹扬光康华丽邓梦杨
第三军医大学新桥医院心血管内科,全军心血管病研究所
Author(s):
FANG Yu-qiang HUANG Lan ZHAO Xiao-hui YIN Yang-guang KANG Hua-li DENG Meng-yang
Institute of Cardiovascular Diseases, Xinqiao Hospital, Third Military Medical University, Chongqing 400037, China
关键词:
 内皮脂肪酶HUVECICAM-1TNF-α
Keywords:
endothelial lipase HUVEC ICAM-1 TNF-α
分类号:
Q556; R322.123; R392.11
文献标志码:
A
摘要:
目的    研究内皮脂肪酶(endothelial lipase, EL)对人脐静脉内皮细胞(human umbilical vein endothelial cells, HUVEC)黏附分子ICAM-1表达的影响及量效关系。    方法    以TNF-α(10ng/ml)刺激HUVEC,用RT-PCR检测黏附分子ICAM-1 mRNA表达的变化;以50 ng/ml EL抗体进行预处理,再检测黏附分子mRNA表达的变化;再用0~50 ng/ml不同浓度EL抗体对HUVEC进行预处理,检测不同浓度EL抗体作用后ICAM-1 mRNA表达的变化。    结果    HUVEC受TNF-α作用后, ICAM-1表达显著增加(P<0.01),该作用可为EL抗体所拮抗,这种拮抗作用呈现剂量依赖性增强(P<0.01,P<0.05)。    结论    EL可呈剂量依赖性的促进内皮细胞表达ICAM-1,提示TNF-α作用于HUVEC产生的EL可促进黏附分子ICAM-1的表达。
Abstract:
Objective    Endothelial lipase (EL) modulates the monocyte adhesion to the endothelial cells. We explore the effect of EL on the expression of adhesion molecules in endothelial cells.     Methods    Human umbilical vein endothelial cells (HUVECs) were treated with TNF-α (10 ng/ml), TNF-α (10 ng/ml)+anti-EL antibody (0, 2.5, 5, 10, 25, 50 ng/ml, respectively) or serum-free media, then the expression levels of intercellular adhesion molecule-1 (ICAM-1) mRNA were detected by RT-PCR.     Results    After treated with TNF-α, the ICAM-1 mRNA levels expressed by HUVECs were significantly increased. These effects were significantly antagonized by anti-EL antibody in a dose-dependent manner.      Conclusion    EL expressed in HUVECs stimulated by TNF-α can promote the expressions of adhesion molecules in endothelial cells, indicating EL may participate in  the pathophysiologic progress of coronary artery disease.

参考文献/References:

方玉强,黄岚,赵晓辉,等.   内皮脂肪酶对人脐静脉内皮细胞ICAM-1表达的影响及量效关系[J]. 第三军医大学学报, 2008, 30(17):1615-1617.

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更新日期/Last Update: 2008-09-16