[1]唐仕明,何大朋,王忠,等.抑制Kit信号通路对豚鼠膀胱间质细胞增殖及自主节律性运动的影响[J].第三军医大学学报,2007,29(23):2264-2266.
 TANG Shi-ming,HE Da-peng,WANG Zhong,et al.Effects of inhibiting Kit signal pathway on proliferation and spontaneous rhythmic motility of interstitial cells in bladder of guinea pigs[J].J Third Mil Med Univ,2007,29(23):2264-2266.
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《第三军医大学学报》[ISSN:1000-5404/CN:51-1095/R]

卷:
29卷
期数:
2007年第23期
页码:
2264-2266
栏目:
论著
出版日期:
2007-12-15

文章信息/Info

Title:
Effects of inhibiting Kit signal pathway on proliferation and spontaneous rhythmic motility of interstitial cells in bladder of guinea pigs
作者:
唐仕明何大朋王忠辜福贤王明荣
四川省广元市人民医院泌尿外科;第三军医大学西南医院胸心外科
Author(s):
TANG Shi-ming HE Da-peng WANG Zhong GU Fu-xian WANG Ming-rong
Department of Urinary Surgery, Guangyuan People’s Hospital; Department of Cardiothoracic Surgery, Southwest Hospital, Third Military Medical University
关键词:
Kit蛋白间质细胞伊马替尼
Keywords:
Kit protein interstitial cell Imatinib
分类号:
R322.62; R329.28
文献标志码:
A
摘要:
目的  探讨抑制Kit表达对豚鼠膀胱间质细胞(interstitial cells, ICs)增殖及自主节律性运动的影响。  方法   成年豚鼠分别予伊马替尼灌胃20 d和40 d,免疫荧光、Western blot和体外灌流观察间质细胞形态和功能的变化。  结果  正常豚鼠膀胱壁内可见较多的间质细胞,用药20 d和40 d后,细胞数量逐渐减少,Kit蛋白的表达逐渐降低,同时,膀胱的自主节律性运动受到抑制。  结论  膀胱内含间质细胞,这些细胞可能是膀胱运动的起搏细胞,其细胞表面表达的Kit蛋白对间质细胞的存活和功能维持都具有重要的作用。
Abstract:
Objective  To investigate the role of Kit signal pathway in the proliferation and spontaneous rhythmic motility of interstitial cells (ICs) in the bladder of guinea pigs.   Methods  Fourteen Guinea pigs aged 45-60 days were administered with Imatinib intragastrically for 20 and 40 days. Three were fed on normal saline as control. The morph and number of ICs were observed by immunofluorescence, Kit protein expression was detected by Western blot and spontaneous rhythmic motility of bladder were investigated in in vitro organ bath.   Results  Quite a few ICs were observed in the bladder of controls. After administration of Imatinib, the amount of ICs and the expression of Kit protein decreased gradually, and the spontaneous rhythmic contraction weakened.   Conclusion  ICs might be the pacemaker of bladder in guinea pigs. Kit protein might play an important role in the survival and functional maintenance of ICs.

参考文献/References:

唐仕明,何大朋,王忠,等.抑制Kit信号通路对豚鼠膀胱间质细胞增殖及自主节律性运动的影响[J].第三军医大学学报,2007,29(23):2264-2266.

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更新日期/Last Update: 2008-05-23