[1]申丽丽,彭家和,刘红,等.法尼酯X受体对脂肪酸合酶表达的影响[J].陆军军医大学学报(原第三军医大学学报),2007,29(22):2142-2144.
　SHEN Li-li,PENG Jia-he,LIU Hong,et al.Effects of farnesoid X receptor on expression of fatty acid synthetase in HepG2 cells[J].J Amry Med Univ (J Third Mil Med Univ),2007,29(22):2142-2144.
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陆军军医大学学报（原第三军医大学学报）[ISSN:1000-5404/CN:51-1095/R]

卷:
29卷
期数:
2007年第22期
页码:
2142-2144
栏目:
论著
出版日期:
2007-11-30

文章信息/Info

Title:
Effects of farnesoid X receptor on expression of fatty acid synthetase in HepG2 cells
作者:
申丽丽; 彭家和; 刘红; 彭彦孟; 甘淋; 鲁立; 张迁; 江渝
第三军医大学：基础医学部生物化学与分子生物学教研室，学员旅，新桥医院血液内科
Author(s):
SHEN  Li-li;  PENG Jia-he;  LIU Hong;  PENG Yan-meng;  GAN Lin;  LU Li;  ZHANG Qian;  JIANG Yu
Department of Biochemistry and Molecular Biology, College of Medicine, Administration Office of Undergraduates, Third Military Medical University, Chongqing 400038; Department of Hematology, Xinqiao Hospital, Third Military Medical University, Chongqing 400037, China
关键词:
法尼酯X受体; 脂肪酸合酶; 动脉粥样硬化; 甘油三酯; 胆汁酸
Keywords:
farnesoid X receptor;  fatty acid synthetase;  ;  atherosclerosis
分类号:
Q591.5;R394;R541.402
文献标志码:
A
摘要:
目的  探讨法尼酯X受体(farnesoid X receptor,FXR)对脂肪酸合酶（fatty acid synthetase, FAS）表达的影响。 方法  用FXR激动剂CDCA作用人肝癌细胞株（HepG2），应用 RT-PCR和Western blotting分别从mRNA水平和蛋白质水平检测FAS的表达情况。 结果  用不同浓度的CDCA（25、50、75 μmol/L）分别作用于HepG2细胞，6、12、24、48 h后，FAS mRNA和蛋白质水平呈时间和剂量依赖性下调。 结论  FXR激动剂可抑制脂肪酸合酶的表达。
Abstract:
Objective    To investigate the effect of the farnesoid X receptor (FXR) on fatty acid synthetase expression.       Methods    Human hepatoblastoma HepG2 cells were treated with a FXR’s agonist, chenodeoxycholic acid (CDCA) at 75 μmol/L for 6, 12, 24 and 48 h respectively, or at 25, 50 and 75 μmol/L for 48 h. Expressions of FAS mRNA and protein were determined by RT-PCR and Western blot analysis respectively.     Results    RT-PCR and Western blot analysis indicated that the mRNA and protein were expressed in a time- and dose-dependent manner in the HepG2 cells treatmented by CDCA.       Conclusion    FXR agonist decreases the expression of fatty acid synthetase.

相似文献/References:

[1]娄桂予,周度金.法尼酯X受体的研究进展[J].陆军军医大学学报(原第三军医大学学报),2005,27(20):2088.
[2]董金瑜,刘红,彭家和,等.法尼酯X受体可下调肝细胞中肝型脂肪酸结合蛋白的表达[J].陆军军医大学学报(原第三军医大学学报),2010,32(05):458.
　Dong Jinyu,Liu Hong,Peng Jiahe,et al.Farnesoid X receptor down-regulates expression of liver fatty acid-binding protein in liver cells[J].J Amry Med Univ (J Third Mil Med Univ),2010,32(22):458.
[3]王永超,刘红,彭家和,等.法尼酯X受体上调肝细胞中成纤维细胞生长因子21的表达[J].陆军军医大学学报(原第三军医大学学报),2011,33(09):912.
　Wang Yongchao,Liu Hong,Peng Jiahe,et al.Farnesoid X receptor upregulates fibroblast growth factor 21 expression in cultured liver cells[J].J Amry Med Univ (J Third Mil Med Univ),2011,33(22):912.
[4]俞慧宏,沈薇,郭进军.与肝X受体相关的脂肪酸合酶转录调控区乙酰化组蛋白动态表达[J].陆军军医大学学报(原第三军医大学学报),2012,34(01):54.
　Yu Huihong,Shen Wei,Guo Jinjun.Dynamic expression of acetylated histone in transcription regulation area of fatty acid synthase related to liver X receptor[J].J Amry Med Univ (J Third Mil Med Univ),2012,34(22):54.

更新日期/Last Update: 2008-07-01