[1]刘晓红,曾俊伟,张金海,等.神经病理性痛大鼠背根神经节P2X3受体表达及电生理学特性的改变[J].第三军医大学学报,2008,30(04):288-291.
 LIU Xiao-hong,ZENG Jun-wei,ZHANG Jin-hai,et al.Dorsal root ganglion P2X3 receptor expression and electrophysiological characteristics in rats with neuropathic pain[J].J Third Mil Med Univ,2008,30(04):288-291.
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《第三军医大学学报》[ISSN:1000-5404/CN:51-1095/R]

卷:
30卷
期数:
2008年第04期
页码:
288-291
栏目:
论著
出版日期:
2008-02-28

文章信息/Info

Title:
Dorsal root ganglion P2X3 receptor expression and electrophysiological characteristics in rats with neuropathic pain
作者:
刘晓红曾俊伟张金海阮怀珍
第三军医大学基础医学部神经生物学教研室,重庆市神经科学研究所;遵义医学院生理学教研室
Author(s):
LIU Xiao-hong ZENG Jun-wei ZHANG Jin-hai RUAN Huai-zhen
Department of Neurobiology, College of Medicine, Third Military Medical University; Department of Physiology, Zunyi Medical College
关键词:
神经损伤背根神经节 P2X受体膜片钳ATP
Keywords:
nerve injury dorsal root ganglion P2X receptor patch clamp ATP
分类号:
R329.25;R338.8;R741.02
文献标志码:
A
摘要:
目的 观察坐骨神经缩窄性损伤(CCI)致神经病理性痛后大鼠背根节神经元P2X3受体的表达和电生理学特性的变化。  方法 应用免疫组化技术观察CCI后不同时间相应节段(L4、L5、L6)脊髓及背根神经节P2X3受体表达的变化;全细胞膜片钳技术观察CCI后不同时间L4、L5、L6节段背根节神经元ATP反应电流的变化。  结果 CCI后7、14 d,损伤同侧L4、L5、L6节段背根节P2X3免疫阳性神经元数量明显增加,而且染色加深,相应节段的脊髓背角浅层P2X3免疫反应性亦明显增加。CCI后7、14 d,损伤同侧相应节段背根节产生ATP快反应电流的细胞数量明显增加,电流幅度亦增强。应用非选择性P2X受体拮抗剂Suramin能明显抑制ATP反应电流。  结论 坐骨神经缩窄性损伤致神经痛后同侧对应节段的背根节神经元P2X3受体表达明显增加, ATP快反应电流明显增强。
Abstract:
Objective To investigate the P2X3 receptor expression and electrophysiological characteristics in dorsal root ganglion (DRG) in rat with neuropathic pain caused by chronic constriction injury of sciatic nerve (CCI).  Methods P2X3 receptor expressions in L4, L5and L6 DRG following CCI were observed by using a polyclonal antibody to label the P2X3 receptor. ATP-activated currents in corresponding DRG neurons following CCI were observed by using electrophysiological technique.  Results A significant increase in P2X3 immunoreactivity was observed in the ipsilateral (injured) L4, L5 and L6DRG and spinal cord on 7, 14 d after CCI. In small diameter neurons, a significant increase in the number of cells exhibiting a transient current to ATP was observed on 7, 14 d after CCI. Moreover, amplitude of these currents was increased.  Conclusion After CCI, the expression and function of P2X3 receptor in corresponding DRG are increased.

参考文献/References:

刘晓红, 曾俊伟, 张金海,等. 神经病理性痛大鼠背根神经节P2X3受体表达及电生理学特性的改变[J].第三军医大学学报,2008,30(4):288-291.

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更新日期/Last Update: 2008-04-29