[1]姚声涛,唐文渊,郭川.LRIG1诱导人胶质瘤细胞凋亡与表皮生长因子受体基因关系[J].陆军军医大学学报(原第三军医大学学报),2008,30(02):157-160.
 YAO Sheng-tao,TANG Wen-yuan,GUO Chuan.Relationship between LRIG1 induced human glioma apoptosis and EGFR gene[J].J Amry Med Univ (J Third Mil Med Univ),2008,30(02):157-160.
点击复制

LRIG1诱导人胶质瘤细胞凋亡与表皮生长因子受体基因关系(/HTML )
分享到:

陆军军医大学学报(原第三军医大学学报)[ISSN:1000-5404/CN:51-1095/R]

卷:
30卷
期数:
2008年第02期
页码:
157-160
栏目:
论著
出版日期:
2008-01-30

文章信息/Info

Title:
Relationship between LRIG1 induced human glioma apoptosis and EGFR gene
作者:
姚声涛唐文渊郭川
重庆医科大学附属第一医院神经外科
Author(s):
YAO Sheng-tao TANG Wen-yuan GUO Chuan
Department of Neurosurgery, The Affiliated First Hospital of Chongqing Medical University
关键词:
胶质瘤 LRIG1 表皮生长因子受体
Keywords:
glioma LRIG1 epidermal growth factor receptor
分类号:
R73-362;R730.23;R730.264
文献标志码:
A
摘要:
目的 研究LRIG1诱导神经胶质瘤细胞的凋亡作用,探讨LRIG1对表皮生长因子受体信号转导通路抑制效应的分子机制。  方法 采用Lipofectamine介导的基因转染技术,将质粒pcDNA3.1-LRIG1转染原代神经胶质瘤细胞,应用逆转录聚合酶链反应(RT-PCR)和Western blot方法检测转染后胶质瘤细胞LRIG1和EGFR mRNA与蛋白水平的变化,Western blot方法检测神经胶质瘤细胞PKCα、Bax、bcl-2蛋白水平的变化。MTT法和流式细胞技术分析细胞的增殖和凋亡的变化。  结果 细胞中转染pcDNA3.1-LRIG1组中以LRIG1 mRNA和蛋白质的表达水平较未处理组和转染空载体组明显升高,而EGFR mRNA和蛋白质的表达水平较对照组和转染空载体组明显降低,PKCα、Bax表达上调,bc1-2表达下降,胶质瘤细胞生长受到抑制,凋亡显著增强。  结论 LRIG1可能通过参与形成EGFR的负反馈环,从多种途径抑制了肿瘤的生长。
Abstract:
Objective To explore the molecular mechanism that LRIG1 inhibits signal transduction system of epidermal growth factor receptor (EGFR) by investigating the role of LRIG1 in glioma.   Methods The plasmid pcDNA3.1-LRIG1 was transfected into primary glioma cells by Lipofectamine. Then, the changes of LRJG1 and EGFR in the transfected glioma cells were measured by RT-PCR and Western blot, and the cell proliferation and apoptosis were analyzed by MTT and flow cytometry.    Results The expression levels of LRIG1 mRNA and protein in the glioma cells transfected with pcDNA-LRIG1 were significantly higher than those of control group and pcDNA3.1 transfected glioma cells, while those of EGFR mRNA and protein were significantly lower. The expression of PKCα and Bax was up-regulated, while the expression of bcl-2 was down-regulated. The growth of glioma cells was inhibited and their apoptosis was obviously enhanced.    Conclusion By participating the construction of the negative feedback loop of EGFR, LRIG1 inhibits the occurrence and growth of tumor through several pathways.

相似文献/References:

[1]陈松,程远,廖鹏,等.S100A9真核表达载体pEGFP-N3-S100A9的构建及其对U251细胞生长的影响[J].陆军军医大学学报(原第三军医大学学报),2012,34(16):1596.
 Chen Song,Cheng Yuan,Liao Peng,et al.Construction of S100A9 eukaryotic expression vector and its effect on proliferation of glioma cell line U251[J].J Amry Med Univ (J Third Mil Med Univ),2012,34(02):1596.
[2]姚一搏,许民辉,徐伦山,等.1H-MRS引导辅助伽玛刀治疗20例脑胶质瘤的初步观察[J].陆军军医大学学报(原第三军医大学学报),2012,34(18):1881.
 Yao Yibo,Xu Minhui,Xu Lunshan,et al.Preliminary observation on the effect of Gamma Knife treatment for 20 glioma patients with 1H-MRS guidance[J].J Amry Med Univ (J Third Mil Med Univ),2012,34(02):1881.
[3]王跃红,赵文清,李文玲,等.白介素-24抑制胶质瘤细胞生长的体内外实验研究[J].陆军军医大学学报(原第三军医大学学报),2008,30(03):229.
 WANG Yue-hong,ZHAO Wen-qing,LI Wen-ling,et al.Interleukin 24 suppresses the growth of malignant glioma cells in vitro and in vivo[J].J Amry Med Univ (J Third Mil Med Univ),2008,30(02):229.
[4]张启川,张冬,廖翠薇,等.弥散加权成像对胶质瘤和脑膜瘤的诊断价值[J].陆军军医大学学报(原第三军医大学学报),2008,30(16):1522.
 ZHANG Qi-chuan,ZHANG Dong,LIAO Cui-wei,et al.Clinical application of DWI in diagnosis of glioma and meningioma[J].J Amry Med Univ (J Third Mil Med Univ),2008,30(02):1522.
[5]王博,王毅,陈金华,等.大鼠C6脑胶质瘤生长规律的影像学与病理学观察[J].陆军军医大学学报(原第三军医大学学报),2008,30(20):1940.
 WANG Bo,WANG Yi,CHEN Jin-hua,et al.Growth pattern of rat C6 brain gliomas: imaging and histopathologic observation[J].J Amry Med Univ (J Third Mil Med Univ),2008,30(02):1940.
[6]王舒楠,王毅,李雪,等.大鼠C6脑胶质瘤血管生成的多层螺旋CT灌注成像研究[J].陆军军医大学学报(原第三军医大学学报),2008,30(20):1944.
 WANG Shu-nan,WANG Yi,LI Xue,et al.Study on angiogenesis of C6 brain gliomas in rats by MSCT perfusion imaging[J].J Amry Med Univ (J Third Mil Med Univ),2008,30(02):1944.
[7]冯玉慧,卞修武,郭德玉,等.在人恶性胶质瘤原代培养细胞甲酰化肽受体FPR的表达和功能意义[J].陆军军医大学学报(原第三军医大学学报),2006,28(24):2440.
[8]程迎新,高原,唐文渊,等.垂体瘤转化基因反义寡核苷酸抑制胶质瘤增殖的实验研究[J].陆军军医大学学报(原第三军医大学学报),2006,28(19):1962.
[9]程迎新,唐文渊.恶性胶质瘤患者外周血淋巴细胞基因表达谱的研究[J].陆军军医大学学报(原第三军医大学学报),2006,28(16):1707.
[10]王清良,卞修武,蒋雪峰,等.计算机辅助的人脑间变性星形细胞瘤微血管构筑三维重建[J].陆军军医大学学报(原第三军医大学学报),2006,28(11):1148.

更新日期/Last Update: 2008-04-30