[1]秦艳,王娅兰,李圆圆.PARP抑制剂对小鼠结肠腺癌CT26细胞肝转移的影响[J].第三军医大学学报,2008,30(14):1330-1333.
 QIN Yan,WANG Ya-lan,LI Yuan-yuan.Effect of poly(ADP-ribose)polymerase inhibitor on liver metastasis of colorectal carcinoma CT26 cell line in mice[J].J Third Mil Med Univ,2008,30(14):1330-1333.
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《第三军医大学学报》[ISSN:1000-5404/CN:51-1095/R]

卷:
30卷
期数:
2008年第14期
页码:
1330-1333
栏目:
论著
出版日期:
2008-07-30

文章信息/Info

Title:
Effect of poly(ADP-ribose)polymerase inhibitor on liver metastasis of colorectal carcinoma CT26 cell line in mice
作者:
秦艳王娅兰李圆圆
重庆医科大学基础医学院病理学教研室
Author(s):
QIN Yan WANG Ya-lan LI Yuan-yuan
Department of Pathology, Faculty of Basic Medical Sciences, Chongqing Medical University, Chongqing 400016, China
关键词:
聚腺苷二磷酸核糖聚合酶(PARP) 5-AIQ肝转移结肠癌
Keywords:
poly(ADP-ribose)polymerase 5-aminoisoquinolinone liver  metastasis colorectal carcinoma
分类号:
R73-36;R73-37;R735.35
文献标志码:
A
摘要:
目的  探讨5-AIQ抑制小鼠结肠癌CT26细胞聚腺苷二磷酸核糖聚合酶[poly(ADP-ribose)polymerase, PARP]后对其肝转移的影响。  方法  以小鼠脾脏接种小鼠结肠癌CT26细胞肝转移为模型,采用CT26细胞体外药物处理后脾脏接种和脾脏接种后直接腹腔给药两种方式,将36只BALB/c小鼠按随机区组设计分成6组,其中4组做实验组,2组做对照组,每组6只。观察脾脏原发肿瘤和肝脏转移肿瘤的变化。Western blot检测PARP在各组脾脏原发瘤组织中的蛋白表达量。  结果  经过药物5-AIQ处理后,CT26细胞脾脏接种的小鼠和CT26细胞直接脾脏接种后腹腔给药处理的小鼠,脾脏肿瘤体积缩小和肝脏转移结节数目及分级减少,与对照组比较差别显著(P<0.05),但是不同剂量之间未见明显差异(P>0.05)。各5-AIQ处理组小鼠脾脏组织PARP表达明显低于未给药处理小鼠脾脏原发瘤组织(P<0.05)。 结论  5-AIQ能抑制CT26细胞PARP表达,对结肠癌CT26细胞脾脏原发瘤的生长及肝转移具有一定的抑制作用。
Abstract:
Objective    To investigate the effect of poly (ADP-ribose)polymerase (PARP) expression inhibited by 5-AIQ on the liver metastasis in colorectal carcinoma CT26 cell lines.     Methods    Liver metastasis model of colorectal carcinoma was established by intrasplenic inoculation of colorectal carcinoma CT26 cell in BALB/c mice. Treated by 5-AIQ (100 or 500 μmol/L),  CT26 cells were inoculated to the spleen of the mice, or the rats were first inoculated of CT26 cells, then treated by  5-AIQ (3 or 10 mg/kg) ip.  The changes spleen primary carcinoma and liver metastasis carcinoma were observed.  The PARP expression in spleen primary carcinoma was detected by Western blotting.     Results    Significant difference was found in the splenic carcinoma size, the number of liver metastatic nodules, the grading of liver metastatic nodules in the 5-AIQ treated group and control group(P<0.05), but no statistical significance was observed in the mice treated with different drug dosages (P>0.05).  The PARP expression of spleen primary carcinoma in 5-AIQ treated groups was lower significantly than that in control group (P<0.05).      Conclusion    5-AIQ can inhibit PARP expression in the CT26 cell lines and the growth of  primary splenic carcinoma and liver metastasis after CT26 cell inoculation in vivo.

参考文献/References:

秦艳,王娅兰,李圆圆.  PARP抑制剂对小鼠结肠腺癌CT26细胞肝转移的影响[J].第三军医大学学报,2008,30(14):1330-1333.

更新日期/Last Update: 2008-07-21