[1]王丽雁,蔡文琴,陈鹏慧.新生大鼠少突胶质前体细胞的培养与鉴定[J].陆军军医大学学报(原第三军医大学学报),2008,30(06):489-491.
 WANG Li-yan,CAI Wen-qin,CHEN Peng-hui.Isolation and identification of oligodendrocyte progenitor cells from neonatal rats[J].J Amry Med Univ (J Third Mil Med Univ),2008,30(06):489-491.
点击复制

新生大鼠少突胶质前体细胞的培养与鉴定(/HTML )
分享到:

陆军军医大学学报(原第三军医大学学报)[ISSN:1000-5404/CN:51-1095/R]

卷:
30卷
期数:
2008年第06期
页码:
489-491
栏目:
论著
出版日期:
2008-03-30

文章信息/Info

Title:
Isolation and identification of oligodendrocyte progenitor cells from neonatal rats
文章编号:
1000-5404(2008)06-0489-03
作者:
王丽雁蔡文琴陈鹏慧
第三军医大学基础医学部神经生物学教研室,重庆市神经科学研究所
Author(s):
WANG Li-yan CAI Wen-qin CHEN Peng-hui
Department of Neurobiology, College of Medicine, Third Military Medical University
关键词:
少突胶质前体细胞细胞培养大鼠
Keywords:
oligodendrocyte progenitor cells cell cultures rat
分类号:
R329-33;R329.2;R338
文献标志码:
A
摘要:
目的 在体外培养条件下获取纯度较高的少突胶质前体细胞(oligodendrocyte progenitor cells, OPCs)。方法 原代混合胶质培养,利用振荡法和差速贴壁法分离纯化少突胶质前体细胞,再用无血清化学条件培养基培养。免疫荧光法及膜片钳记录细胞电生理特性进行鉴定。结果 振荡分离后超过90 %细胞为NG2阳性OPCs;OPCs电生理特性:输入阻抗Ra相对较高;电压依赖的K+电流具有外向整流特性;对河豚毒素(tetrodotoxin, TTX)敏感的小的内向Na+流,不能产生动作电位。结论 振荡法结合差速贴壁可以获得纯度较高的OPCs;OPCs具有区别于神经元、星型胶质细胞、小胶质细胞的独有电生理特性。
Abstract:
Objective To develop new methods to cultivate, retrieve and purify oligodendrocyte progenitor cells (OPCs).  Methods Primary OPCs were isolated from mixed glial cultures of newborn Sprague Dawley rat forebrains by using a selective detachment procedure, further purified by differential adhesion, and maintained in a chemical conditioned medium. The characteristic of OPCs was determined by both electrophysiological recording and immunostaining.  Results Immunocytochemical analysis revealed that more than 90% of the isolated cells expressed the OPCs-specific marker NG2. OPCs exhibited a moderate input resistance, delayed rectifier K+ currents (IK) that could be antagonized partly by TEA-Cl and CsCl, small tetrodotoxin (TTX) sensitive Na+ currents that failed to generate typical action potentials.  Conclusion Isolation and purification by selective detachment and differential adhesion procedure are effective to obtain highly purified OPCs. OPCs, distinct from neuron, astrocyte, oligodendrocyte or microglia, possess unique physiological properties.

相似文献/References:

[1]易斌,钱桂生,白莉,等.不同方法培养大鼠肺动脉平滑肌细胞的差异[J].陆军军医大学学报(原第三军医大学学报),2005,27(24):2425.
[2]夏珊,李世荣,刘剑毅,等.大黄素对人增生性瘢痕成纤维细胞的增殖抑制及细胞内游离Ca2+的作用[J].陆军军医大学学报(原第三军医大学学报),2007,29(20):1982.
 XIA Shan,LI Shi-rong,LIU Jian-yi,et al.Effect of emodin on proliferation and intracellular free calcium of hypertrophic scar fibroblasts in vitro[J].J Amry Med Univ (J Third Mil Med Univ),2007,29(06):1982.
[3]桂伶俐,张传汉,刘志恒,等.不同胚龄人神经前体细胞分离培养及分化特性的比较[J].陆军军医大学学报(原第三军医大学学报),2007,29(15):1469.
 GUI Ling-li,ZHANG Chuan-han,LIU Zhi-heng,et al.Culture and differentiated characteristics of human neural progenitor cells derived from embryonic brains of different ages[J].J Amry Med Univ (J Third Mil Med Univ),2007,29(06):1469.
[4]路菊,赵建华,郭乔楠,等.细胞与生物材料共培养的样品扫描电镜制备法[J].陆军军医大学学报(原第三军医大学学报),2007,29(14):1446.
[5]刘志能,姚珍薇,唐良萏,等.异位和正常子宫内膜间质细胞对上皮细胞间隙连接细胞间通讯的影响[J].陆军军医大学学报(原第三军医大学学报),2007,29(20):1972.
 LIU Zhi-neng,YAO Zhen-wei,TANG Liang-dan,et al.Influence of ectopic and normal endometrial stromal cells on GJIC in epithelial cells in vitro[J].J Amry Med Univ (J Third Mil Med Univ),2007,29(06):1972.
[6]刘恺鸣,迟路湘,鲁向辉,等.大鼠脑微血管内皮细胞的原代培养[J].陆军军医大学学报(原第三军医大学学报),2007,29(20):2011.
[7]李捷,张宜明,刘颖,等.抗坏血酸诱导体外培养小鼠胚胎干细胞分化为心肌细胞[J].陆军军医大学学报(原第三军医大学学报),2008,30(18):1722.
 LI Jie,ZHANG Yi-ming,LIU Ying,et al.Ascorbic acid induces mouse embryonic stem cells to differentiate into cardiac myocytes[J].J Amry Med Univ (J Third Mil Med Univ),2008,30(06):1722.
[8]季亢挺,张怀勤,杨鹏麟,等.不同细胞生长因子组合对培养的内皮祖细胞集落形成及黏附能力的影响[J].陆军军医大学学报(原第三军医大学学报),2006,28(24):2468.
[9]金世龙,刘宝华,刘宏鸣,等.大鼠成体肝卵圆细胞分离培养及诱导分化的初步研究[J].陆军军医大学学报(原第三军医大学学报),2006,28(22):2259.
[10]朱瑾,陈平,李晓武,等.原代培养大鼠肝窦内皮细胞凋亡的检测及意义[J].陆军军医大学学报(原第三军医大学学报),2006,28(06):515.

更新日期/Last Update: 2008-04-07