[1]杨光伦,姚榛祥,黄平,等.来氟米特在大鼠异种心脏移植排斥反应中对NF-κB P65的影响[J].第三军医大学学报,2007,29(23):2267-2270.
 YANG Guang-lun,YAO Zhen-xiang,HUANG Ping,et al.Effect of leflunomide on NF-κB P65 in mouse to rat cardiac xenograft[J].J Third Mil Med Univ,2007,29(23):2267-2270.
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《第三军医大学学报》[ISSN:1000-5404/CN:51-1095/R]

卷:
29卷
期数:
2007年第23期
页码:
2267-2270
栏目:
论著
出版日期:
2007-12-15

文章信息/Info

Title:
Effect of leflunomide on NF-κB P65 in mouse to rat cardiac xenograft
作者:
杨光伦姚榛祥黄平涂刚
重庆医科大学附属第一医院普通外科
Author(s):
YANG Guang-lun YAO Zhen-xiang HUANG Ping TU Gang
Department of General Surgery, The First Affiliated Hospital of Chongqing Medical University
关键词:
异种移植心脏移植免疫抑制剂NF-κB
Keywords:
xenotransplantation heart transplantation immunosuppressant NF-κB
分类号:
R392.4; R654.2; R979.5
文献标志码:
A
摘要:
目的 复制小鼠到大鼠异种心脏移植模型,探讨来氟米特(leflunomide, Lef)在大鼠异种心脏移植排斥反应中对NFκB P65的影响。 方法 实验动物按随机数字表法分成4组,每组6只:空白移植组、环孢素A(CsA)组、来氟米特(Lef)组、Lef+ CsA组。采用免疫组化法和蛋白印迹杂交法检测各组移植心肌组织中NF-κB P65的表达;凝胶电泳迁移率法检测NF-κB DNA结合活性。 结果 单用CsA不能延长移植心脏存活时间[(2.50±1.05)d],也不能抑制移植心肌NFκB P65蛋白的表达(263.09±28.81,IOD)和NF-κB DNA结合活性(227.49±14.83,IOD);而单用Lef可显著延长移植心脏存活时间[(4.17±1.33)d],也能显著抑制移植心肌NF-κB P65蛋白的表达(173.48±5.85,IOD)和NF-κB DNA结合活性(109.96±12.46,IOD),与空白移植和CsA组比较,均有显著性差异(P<0.05)。Lef 与CsA联合应用使移植心脏存活(6.50±2.56)d,抑制移植心肌组织中NF-κB P65蛋白的表达(67.79±3.79,IOD)和NF-κB DNA结合活性(43.61±7.42,IOD)最为显著,与其他3组比较,均有显著性差异(P<0.05)。 结论 在大鼠心脏异种移植中Lef与CsA联用可通过显著抑制NF-κB P65蛋白表达和NF-κB DNA结合活性来延长移植心脏的存活时间,二者具有免疫协同作用。
Abstract:
Objective  By reproducing mouse to rat heart xenograft, to determine the effect of leflunomide on NF-κB P65 in the transplanted heart.   Methods  The recipient rats were randomly divided into 4 groups: group A (n=6) receiving no immunosuppressant as control; group B (n=6) cyclosporine (CsA); group C (n=6) leflunomide (Lef), group D (n=6) Lef plus CsA. The expressions of NF-κB P65 protein and NF-κB P65 DNA binding activity in transplanted heart tissues were determined by immunohistochemistry, Western blot and electrophoretic mobility shift assay (EMSA).   Results  Administration of CsA alone had no appreciable effect on the survival time of xenografts [(2.50±1.05) d] and could not suppress the protein expression of NF-κB P65 [(263.09±28.81), IOD] and NF-κB P65 DNA binding activity [(227.49±14.83), IOD] in the tissues of xenografts. Administration of Lef alone could significantly prolong the survival time of xenografts [(4.17±1.33) d] and could suppress the protein expression of NF-κB P65 [(173.48±585), IOD] and NF-κB P65 DNA binding activity [(109.96±12.46), IOD] (P<0.05, vs group A and group B respectively). Lef plus CsA could significantly prolong the survival time of xenografts to (6.50±2.56) d and suppress the protein expression of NF-κB P65 [(67.79±3.79), IOD] and NF-κB P65 DNA binding activity [(43.61±7.42), IOD] (P<0.05, vs group A, B and group C respectively).    Conclusion  Combination of Lef and CsA can significantly prolong the survival time of xenograft through suppressing the protein expression of NF-κB P65 and NF-κB P65 DNA binding activity in the tissues of transplanted hearts and exerts synergistic immunosuppressive effect in mouse to rat cardiac xenograft.

参考文献/References:

杨光伦,姚榛祥,黄平,等.来氟米特在大鼠异种心脏移植排斥反应中对NF-κB P65的影响[J].第三军医大学学报,2007,29(23):2267-2270.

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更新日期/Last Update: 2008-05-23