[1]赖国旗,邱宗荫,向廷秀,等.肿瘤坏死因子相关凋亡诱导配体诱导HeLa细胞凋亡的差异蛋白质组分析[J].陆军军医大学学报(原第三军医大学学报),2008,30(04):318-321.
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肿瘤坏死因子相关凋亡诱导配体诱导HeLa细胞凋亡的差异蛋白质组分析(/HTML )
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陆军军医大学学报（原第三军医大学学报）[ISSN:1000-5404/CN:51-1095/R]

卷:
30卷
期数:
2008年第04期
页码:
318-321
栏目:
论著
出版日期:
2008-02-28

文章信息/Info

Title:
Differential proteomic analysis of HeLa cells induced by TRAIL
作者:
赖国旗; 邱宗荫; 向廷秀; 宋敏; 胡小龙; 何明忠; 谭冬梅
重庆医科大学：实验动物中心，医学检验系，临床检验诊断学省部共建教育部重点实验室，附属第一医院消化内科，药学院，临床医学一系
Author(s):
LAI Guo-qi;  QIU Zong-yin;  XIANG Ting-xiu;  SONG Min;  HU Xiao-long;  HE Ming-zhong;  TAN Dong-mei
Laboratory Animal Center, Key Laboratory of Clinical Medical Diagnostics, Ministry of Education, Department of Gastroenterology, The First Affiliated Hospital, Department of Pharmacy, Department One of Clinic Medicine, Chongqing Medical University
关键词:
肿瘤坏死因子相关凋亡诱导配体; HeLa细胞; 蛋白质组分析; RT-PCR
Keywords:
TNF-related apoptosis-inducing ligand;  HeLa cells;  Proteomics;  RT-PCR
分类号:
R394-33;R73-362;R737.33
文献标志码:
A
摘要:
目的 比较分析肿瘤坏死因子相关凋亡诱导配体（TNF-related apoptosis-inducing ligand， TRAIL）诱导人宫颈癌细胞系HeLa细胞凋亡前后蛋白质组中的差异表达蛋白，以进一步了解TRAIL的作用机制。  方法 以TRAIL诱导人宫颈癌细胞系HeLa细胞凋亡为模型，用双向电泳技术分离HeLa全细胞蛋白质，MALDI-TOF质谱鉴定，MS-FIit数据库分析，RT-PCR半定量方法检测JNK2的表达。  结果 鉴定得到12个明显的差异蛋白点，其中8个蛋白点上调，4个蛋白点下调。JNK2的表达与TRAIL有良好的量效关系。  结论 JNKK、MAPKAPK-2、Dip13 beta、SHPS-1、DDBb、JEM-1、Macoilin、Leucine-rich repeat-containing protein 14、Ena/VASP-like protein、PTPase-MEG2、GRB10 adaptor protein、THUMP domain-containing protein 1为TRAIL诱导HeLa细胞凋亡的新的相关蛋白。
Abstract:
Objective To detect the differential proteins between HeLa cells treated and untreated by TRAIL so as to further understand the mechanisms of apoptosis induced by TRAIL in HeLa cells.   Methods The differential proteins were observed by 2D electrophoresis, identified by matrixassisted laser desorption/ionizationtime of flightmass spectrometry (MALDI-TOF-MS) and analyzed by MS Fit. The expression of JNK2 was examined by semiquantitative RT-PCR method.   Results There were twelve differential proteins between HeLa cells treated and untreated by TRAIL, 8 up-regulated and 4 down-regulated. There was a good dose-effect relationship between JNK2 and TRAIL.   Conclusion JNKK, MAPKAPK-2, Dip13 beta, SHPS-1, DDBb, JEM-1, Macoilin, Leucine-rich repeat-containing protein 14, Ena/VASP-like protein, PTPase-MEG2, GRB10 adaptor protein, THUMP domain-containing protein 1 take part in the apoptosis of HeLa cells induced by TRAIL.

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更新日期/Last Update: 2008-04-29