[1]冯起甲,徐智,吴国明,等.脂多糖结合蛋白的B细胞抗原表位预测及其效应初步分析[J].第三军医大学学报,2008,30(04):292-294.
 FENG Qi-jia,XU Zhi WU Guo-ming,HU Chuan-min,et al.Prediction of B-cell antigen epitope on lipopolysaccharidebinding protein[J].J Third Mil Med Univ,2008,30(04):292-294.
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《第三军医大学学报》[ISSN:1000-5404/CN:51-1095/R]

卷:
30卷
期数:
2008年第04期
页码:
292-294
栏目:
论著
出版日期:
2008-02-28

文章信息/Info

Title:
Prediction of B-cell antigen epitope on lipopolysaccharidebinding protein
作者:
冯起甲徐智吴国明胡川闽徐顺贵陈维中 郭芮伶
第三军医大学: 新桥医院全军呼吸内科研究所,全军呼吸病研究重点实验室, 医学检验系临床生物化学教研室
Author(s):
FENG Qi-jia XU Zhi WU Guo-ming HU Chuan-min XU Shun-gui CHEN Wei-zhong GUO Rui-ling
Institute of Respiratory Diseases, Xinqiao Hospital,  Department of Clinical Biochemistry, College of Medical Laboratory, Third Military Medical University
关键词:
LBP抗原表位B细胞生物信息学
Keywords:
LBP antigen B cell epitope bioinformatics
分类号:
R318.04;R392.1;R392.2
文献标志码:
A
摘要:
目的 预测人脂多糖结合蛋白(LBP)的B 细胞抗原表位。  方法 采用生物信息软件和互联网服务器,预测LBP的二级结构和分析LBP表面特性(亲水性、可塑性、可及性和抗原性),再计算平均抗原指数(AI),预测LBP 的B细胞抗原表位。  结果 LBP 存在多个潜在的抗原表位,247~260序列(FKGEIFHRNHRSPV)的AI(0.040 7)明显高于其他片段,370~379序列(LPSSSKEPVF)和303~325序列(ITDDMIPPDSNIRLTTKSFRPFV)的AI分别是0.033 6和0.029 5。247~260 序列是LBP 潜在的B 细胞优势抗原表位。  结论 应用多参数预测到LBP的B细胞抗原表位。
Abstract:
Objective To predict the B-cell epitope for lipopolysaccharide-binding protein (LBP) antigen.  Methods Using biotic software and network servers, the secondary structure of LBP was predicted, and the surface properties of LBP, such as hydrophilicity, flexibility, accessibility and antigenicity, were analyzed. Then the average antigen index (AI) was calculated by using Wu’s method.  Results Many distinct antigenic epitopes on LBP were identified: AI of sequence 247-260 (FKGEIFHRNHRSPV) on LBP was 0.040 7, significantly higher than that of other sequences. AI of sequence 370-379 (LPSSSKEPVF) and sequence 303-325 (ITDDMIPPDSNIRLTTKSFRPFV) was 0.033 6 and 0.029 5, respectively. Sequence 247-260 was the prevalent antigen epitope of Bcell on LBP.  Conclusion The B-cell epitopes of LBP is predicted successfully.

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更新日期/Last Update: 2008-04-29