[1]王永明,胡燕,黎海芪.小鼠整合素α4基因克隆、序列分析及突变修复[J].陆军军医大学学报(原第三军医大学学报),2007,29(22):2131-2134.
 WANG Yong-ming,HU Yan,LI Hai-qi.Molecular cloning, sequence analysis of mouse integrin α4 gene and repairing of its mutation[J].J Amry Med Univ (J Third Mil Med Univ),2007,29(22):2131-2134.
点击复制

小鼠整合素α4基因克隆、序列分析及突变修复(/HTML )
分享到:

陆军军医大学学报(原第三军医大学学报)[ISSN:1000-5404/CN:51-1095/R]

卷:
29卷
期数:
2007年第22期
页码:
2131-2134
栏目:
论著
出版日期:
2007-11-30

文章信息/Info

Title:
Molecular cloning, sequence analysis of mouse integrin α4 gene and repairing of its mutation
作者:
王永明胡燕黎海芪
重庆医科大学附属儿童医院儿童保健科
Author(s):
WANG Yong-ming HU Yan LI Hai-qi
Department of Primary Child Care, the Children’s Hospital, Chongqing Medical University,Chongqing 400014, China
关键词:
α4基因基因克隆RT-PCR突变修复
Keywords:
α4gene cDNA clone RT-PCR mutation repairing
分类号:
R322.45;R394-33;R394.3
文献标志码:
A
摘要:
目的  克隆小鼠整合素α4基因cDNA,进行分析整合素α4基因序列,对造成氨基酸突变的碱基进行定点突变修复。  方法  从小鼠小肠peyer’s结获得α4基因cDNA,采用RT-PCR方法,克隆到pMD18-T载体,选择阳性克隆进行酶切鉴定和序列测定,对造成氨基酸突变的碱基进行定点突变修复。  结果  扩增得到的α4基因cDNA为3 099 bp,编码1 032 个氨基酸,与GenBank中公布的序列比较,有12个碱基发生突变,其中6个碱基造成6个氨基酸发生改变,对发生突变的6个碱基进行定点突变修复。  结论  获得小鼠整合素α4基因的克隆。
Abstract:
Objective    To clone and analyze the full-length cDNA of mouse integrin α4, and repair the mutation sensible locei that caused the change of amino acids.     Methods    The cDNA of α4 gene was amplified by RT-PCR using the total RNA extracted from mouse small intestine peyer’s patch. The PCR product was inserted into pMD19-T vector and then transformed into E. coli JM109. The positive recombinant clone was analyzed by restriction endonuclease and DNA sequencing. The mutation of α4cDNA that caused the change of amino acids was repaired.     Results    The cDNA of mouse α4 had a length of 3 099 bp, and encoded a product of 1 032 amino acids. There were 12 bases pairs mutation of α4 gene and the 6 base pairs causing the change of amino acids was repaired.     Conclusion    The cDNA of mouse α4 is cloned successfully.

相似文献/References:

[1]王颖莹,陈纯海,裴莉萍,等.2型大麻受体的克隆及真核表达体系的构建[J].陆军军医大学学报(原第三军医大学学报),2007,29(11):1003.
 WANG Ying-ying,CHEN Chun-hai,PEI Li-ping,et al.Clone and eukaryotic expression of human cannabinoid receptor 2 gene[J].J Amry Med Univ (J Third Mil Med Univ),2007,29(22):1003.
[2]杨俊霞,易发平,宋方洲,等.豚鼠MCHR2基因外显子1的克隆及序列分析[J].陆军军医大学学报(原第三军医大学学报),2006,28(21):2167.
[3]李忠俊,陈幸华.白血病原代骨髓基质细胞诱导Jurkat细胞基因差异表达研究[J].陆军军医大学学报(原第三军医大学学报),2006,28(14):1461.
[4]连继勤,戴旭芳,李晓辉,等.hLNα4LG1基因克隆、表达及生物学活性检测[J].陆军军医大学学报(原第三军医大学学报),2006,28(14):1464.
[5]周静,姜政,孙伟,等.人脂联素真核表达载体的构建及其表达[J].陆军军医大学学报(原第三军医大学学报),2009,31(14):1413.
[6]李成仁,蔡文琴,苏炳银,等.NOV基因真核表达载体的构建及在COS-7细胞中的表达[J].陆军军医大学学报(原第三军医大学学报),2005,27(12):1187.
[7]李树钧,钱桂生,戚好文,等.巨噬细胞炎性蛋白4的非融合表达载体的构建和表达[J].陆军军医大学学报(原第三军医大学学报),2005,27(21):2111.
[8]惠玲,王晓辉,袁碧波,等.钙整合素结合蛋白CIB真核表达载体的构建及在NIH3T3细胞中的表达[J].陆军军医大学学报(原第三军医大学学报),2007,29(03):216.
 HUI Ling,WANG Xiao-hui,YUAN Bi-bo,et al.Construction of eukaryotic expression of CIB vectors and its expression in NIH3T3 cells[J].J Amry Med Univ (J Third Mil Med Univ),2007,29(22):216.
[9]许福明,马文煜,项秉懿,等.淋球菌IgA蛋白酶基因中和表位片段的克隆、表达和鉴定[J].陆军军医大学学报(原第三军医大学学报),2000,22(11):0.[doi:10.16016/j.1000-5404.2000.11.025 ]
[10]谭虎,杨天德,粟永萍,等.修饰GLP2分泌性表达载体的构建及促增殖活性测定的实验研究[J].陆军军医大学学报(原第三军医大学学报),2007,29(17):1650.
 TAN Hu,YANG Tian-de,SU Yong-ping,et al.Construction of secretary expression vector of mutation chimeric GLP2 and detection of its proliferation-promoting activity[J].J Amry Med Univ (J Third Mil Med Univ),2007,29(22):1650.

更新日期/Last Update: 2008-07-01