[1]钟善传,罗雪,陈兴书,等.转录因子EN2在小鼠胚胎组织中的分布[J].陆军军医大学学报(原第三军医大学学报),2008,30(03):192-194.
 ZHONG Shan-chuan,LUO Xue,CHEN Xing-shu,et al.Expression of transcription factor Engrailed-2 in mouse embryo[J].J Amry Med Univ (J Third Mil Med Univ),2008,30(03):192-194.
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转录因子EN2在小鼠胚胎组织中的分布(/HTML )
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陆军军医大学学报(原第三军医大学学报)[ISSN:1000-5404/CN:51-1095/R]

卷:
30卷
期数:
2008年第03期
页码:
192-194
栏目:
论著
出版日期:
2008-02-15

文章信息/Info

Title:
Expression of transcription factor Engrailed-2 in mouse embryo
作者:
钟善传罗雪陈兴书蔡其燕姚忠祥
第三军医大学基础医学部组织学与胚胎学教研室,重庆市神经科学研究所
Author(s):
ZHONG Shan-chuan LUO Xue CHEN Xing-shu CAI Qi-yan YAO Zhong-xiang
Department of Histology and Embryology, College of Medicine, Third Military Medical University, Chongqing Institute of Neuroscience
关键词:
Engrailed-2胚胎发育
Keywords:
Engrailed-2 embryo development
分类号:
R-332;R329.1;R394.2
文献标志码:
A
摘要:
目的 观察转录因子EN2在小鼠胚胎发育过程中的时空分布规律。  方法 利用Western blot技术检测EN2在小鼠胚胎E8.5~E17.5的表达强度;利用免疫组化技术观察EN2在胚胎发育不同时期各组织器官中的分布。  结果 ①Western blot:E8.5未检测到明显的阳性蛋白条带, E9.5~E15.5在相对分子质量为41×103处检测到清晰的EN2蛋白条带,表达水平从E9.5~E12.5逐渐增强,E13.5之后逐渐减弱,至E16.5消失。②免疫组化:在胚胎不同时期,EN2蛋白在中后脑交界部、Rathke囊周围、下颌弓、视交叉、嗅球、椎间盘、轴旁肌和一些间充质组织中都有不同程度的表达。 结论  EN2蛋白可能参与了神经系统、骨骼肌、椎间盘和一些间充质组织的发育与分化。
Abstract:
Objective To investigate the expression pattern of the transcription factor Engrailed-2 (EN2) during mouse embryo development.   Methods The expression level of EN2 during E8.5 (embryo day 8.5) to E17.5 was detected by Western blot and the mapping of EN2 at different embryo day was detected by immunohistochemistry.   Results Western blot analysis showed there were clear straps during E9.5 to E15.5. The expression level of EN2 increased gradually from E9.5 to E13.5, then weakened. Immunohistochemical analysis showed during E9.5 to E15.5, the EN2 protein was detected in mid-hindbrain junction, around the Rathke pouch, in mandibular arch, chiasma opticum, olfactory bulb, discus intervertebrales, dorsolateral muscles and some mesenchymal tissues.  Conclusion The EN2 protein maybe involved in differentiation and development of the tissues and the organs mentioned above.

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更新日期/Last Update: 2008-05-04