[1]孙梯业,赵永亮,颜伟,等.CXCR4在VEGF-C介导的胃癌淋巴道转移中的作用[J].陆军军医大学学报(原第三军医大学学报),2008,30(02):161-165.
 SUN Ti-ye,ZHAO Yong-liang,YAN Wei,et al.Role of CXCR4 in lymph node metastasis promoted by VEGF-C in gastric carcinoma[J].J Amry Med Univ (J Third Mil Med Univ),2008,30(02):161-165.
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CXCR4在VEGF-C介导的胃癌淋巴道转移中的作用(/HTML )
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陆军军医大学学报(原第三军医大学学报)[ISSN:1000-5404/CN:51-1095/R]

卷:
30卷
期数:
2008年第02期
页码:
161-165
栏目:
论著
出版日期:
2008-01-30

文章信息/Info

Title:
Role of CXCR4 in lymph node metastasis promoted by VEGF-C in gastric carcinoma
作者:
孙梯业赵永亮颜伟石彦曾冬竹余佩武
第三军医大学西南医院普通外科;空军总医院妇产科
Author(s):
SUN Ti-ye ZHAO Yong-liang YAN Wei SHI Yan ZENG Dong-zhu YU Pei-wu
Department of General Surgery, Southwest Hospital, Third Military Medical University; General Hospital of Air Force of PLA
关键词:
胃癌CXCR4VEGF-C淋巴转移侵袭转移
Keywords:
gastric carcinoma CXCR4 VEGF-C lymphatic metastasis invasion metastasis
分类号:
R73-362;R73-37;R735.2
文献标志码:
A
摘要:
目的 探索趋化因子受体CXCR4和VEGF-C与胃癌淋巴道转移的关系,为研究干预胃癌淋巴转移的新方法提供理论依据。  方法 采用RT-PCR方法检测86例胃癌组织中趋化因子受体CXCR4 mRNA的表达及VEGF-C mRNA的表达,探索其与胃癌淋巴结转移的关系;Boyden趋化小室法检测CXCR4不同表达状态胃癌细胞的趋化活性和趋化抑制性。  结果 86例胃癌组织均有不同程度的CXCR4 mRNA和VEGF-C mRNA的表达。53例CXCR4 mRNA呈高表达,表达率为61.63%,VEGF-C mRNA高表达率为56.98%(49/86);VEGF-C mRNA高表达组中的CXCR4 mRNA的表达率也较高;CXCR4 mRNA的表达与胃癌淋巴结转移呈正相关(P<0.05);CXCR4的配体SDF-l对胃癌细胞的平均趋化率为81%,经抗CXCR4单克隆抗体处理后癌细胞的平均趋化率下降至30%,CXCR4被特异性抗体封闭前后的癌细胞趋化活性有显著性差异(P<0.05)。  结论 胃癌CXCR4的表达和VEGF-C的表达与胃癌淋巴结转移呈正相关;CXCR4的功能状态影响胃癌细胞的迁移活性。通过干预趋化因子受体CXCR4和VEGF-C的活性可能成为阻断胃癌淋巴转移的新靶点。
Abstract:
Objective To explore the role of chemokine receptor CXCR4 in lymphatic metastasis induced by VEGF-C in gastric carcinoma.   Methods The mRNA expressions of CXCR4 and VEGF-C were determined by reverse transcription PCR (RT-PCR) in 86 cases of primary breast carcinoma, and the migration and inhibition response of CXCR4 to chemokine stromal derived factor-1 (SDF-1 or CXCL12) were detected by Boyden chemotaxis assay.   Results Out of 86 cases, 53 cases presented high expression levels of CXCR4 (61.63%, 53/86), 49 cases showed high expression levels of VEGF-C mRNA (56.98%, 49/86). The cases with higher expression of VEGF-C mRNA presented higher expression of CXCR4 mRNA. The expression of CXCR4 mRNA was positively correlated with lymph node metastasis in primary gastric carcinoma (P<0.05). The average chemotaxis activity of CXCR4 to SDF-1  was evaluated as 81% in gastric cancer cells, but dropped to 30% after using anti-CXCR4 monoclonal antibody, with significant difference (P<0.05).   Conclusion There is a positive correlation between lymphatic metastasis and the expression of CXCR4 and VEGF-C mRNA in primary gastric carcinoma. The functional status of CXCR4 affects the chemotaxis of gastric cancer cells.

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更新日期/Last Update: 2008-04-30