[1]张钢,高钰琪,刘福玉.缺氧对大鼠白细胞粘弹性的影响[J].第三军医大学学报,2008,30(01):46-48.
 ZHANG Gang,GAO Yu-qi,LIU Fu-yu.Effects of hypoxia on viscoelastic properties of polymorphonuclear leukocyte of rats[J].J Third Mil Med Univ,2008,30(01):46-48.
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缺氧对大鼠白细胞粘弹性的影响(/HTML )
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《第三军医大学学报》[ISSN:1000-5404/CN:51-1095/R]

卷:
30卷
期数:
2008年第01期
页码:
46-48
栏目:
论著
出版日期:
2008-01-15

文章信息/Info

Title:
Effects of hypoxia on viscoelastic properties of polymorphonuclear leukocyte of rats
作者:
张钢高钰琪刘福玉
第三军医大学高原军事医学系病理生理学与高原生理学教研室,全军高原生理与高原病研究重点实验室
Author(s):
ZHANG Gang GAO Yu-qi LIU Fu-yu
Department of Pathophysiology, College of High Altitude Military Medicine, Third Military Medical university
关键词:
缺氧白细胞粘弹性
Keywords:
hypoxialeukocyteviscoelasticity
分类号:
R331.142; R361.3; R364.4
文献标志码:
A
摘要:
目的  研究急、慢性缺氧对中性粒细胞粘弹性的影响。  方法 低压舱模拟高原复制缺氧模型,成年Wistar大鼠15只,随机分为常氧组、急性缺氧组(5 000 m减压3 d)和慢性缺氧组(5 000 m减压30 d)。微管吸吮技术分析粒细胞粘弹性变化,标准线性固体模型拟合结果。  结果 常氧组粒细胞弹性模量K1为(39.52±16.06)Pa,K2为(148.85±9.06)Pa,粘性系数μ为(2.28±1.11)Pa·s;急、慢性缺氧组各参数均显著增大(P<0.05),K1为(86.66±26.84) Pa和(179.61±26.31)Pa,K2为(399.89±22.03) Pa和(472.12±30.36)Pa,μ为(4.23±1.82 ) Pa·s和(9.21±2.65)Pa·s;急性缺氧初始变形长度、最大变形长度和时间常数较对照组显著减小(P<0.05),但慢性缺氧组时间常数较对照组已无明显差异(P>0.05)。  结论 急、慢性缺氧对粒细胞生物力学特性有明显影响,表现为细胞膜刚性增大,细胞变形性下降。
Abstract:
Objective  To explore the effects of acute or chronic hypoxia on the viscoelastic properties of polymorphonuclear leukocyte (PMN) of rats.   Methods  Hypobaric chamber was used to simulate high altitude condition. Fifteen adult Wistar rats were randomized into 3 groups: control, acute hypoxia (5 000 m for 3 d) and chronic hypoxia (5 000 m for 30 d). Micropipette aspiration technique was used to evaluate the biomechanical properties of PMNs of rats and the experimental results were fitted with a three-element standard linear solid model.   Results  The viscoelastic coefficients of PMNs in acute hypoxic condition [K1=(86.66±26.84) Pa, K2=(399.89±22.03)Pa,  μ=(4.23±1.82) Pa·s] or those values in chronic hypoxic condition [K1=(179.61±26.31) Pa, K2=(472.12±30.36) Pa, μ=(9.21±2.65) Pa·s] were significantly higher than the corresponding value of PMNs in control(P<0.05) [K1=(39.52±16.06) Pa,K2=(148.85±9.06) Pa,μ=(2.28±1.11) Pa·s]. Compared with the control, the initial strain, maximum strain and the time constant in acute hypoxia was obviously decreased(P<0.05), but the time constant in chronic hypoxia had no difference.   Conclusion  These results suggest that acute or chronic hypoxia has strong impact on the biomechanical properties of PMN, involving the increased rigidity of cell membrane and the decreased deformation of cell.

参考文献/References:

张钢, 高钰琪, 刘福玉. 缺氧对大鼠白细胞粘弹性的影响[J].第三军医大学学报,2008,30(1):46-48.

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更新日期/Last Update: 2008-05-05