[1]任泂,粟永萍,李洪涛,等.JAB1表达下调对LPS诱导炎性因子TNF-α和IL-6的影响[J].第三军医大学学报,2007,29(16):1552-1554.
 REN Jiong,SU Yong-ping,LI Hong-tao,et al.Effect of JAB1 down-regulation on LPS-induced TNF-α and IL-6 in mouse macrophage cells[J].J Third Mil Med Univ,2007,29(16):1552-1554.
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《第三军医大学学报》[ISSN:1000-5404/CN:51-1095/R]

卷:
29卷
期数:
2007年第16期
页码:
1552-1554
栏目:
论著
出版日期:
2007-08-30

文章信息/Info

Title:
Effect of JAB1 down-regulation on LPS-induced TNF-α and IL-6 in mouse macrophage cells
作者:
任泂粟永萍李洪涛李军王军平邹仲敏
第三军医大学军事预防医学院防原医学教研室,全军复合伤研究所,创伤、烧伤与复合伤国家重点实验室
Author(s):
REN Jiong SU Yong-ping LI Hong-tao LI Jun WANG Jun-ping ZOU Zhong-min
State Key Laboratory of Trauma, Burns and Combined Injury, Institute of Combined Injury, College of Preventive Medicine, Third Military Medical University, Chongqing 400038, China
关键词:
JAB1RNA干扰巨噬细胞炎性因子IL-6TNF-α
Keywords:
JAB1RNAimacrophageinflammatory factorIL-6TNF-α
分类号:
R392-33;R392.11;R392.12
文献标志码:
A
摘要:
目的    初步探讨JAB1基因表达下调对炎症反应的影响。     方法    构建特异性针对小鼠JAB1基因的RNA干扰真核表达载体,转染到RAW264.7细胞中降低其JAB1基因的表达,观察LPS刺激后培养上清中炎性因子TNF-α和IL-6的浓度变化。    结果    成功得到小鼠JAB1基因的RNA干扰真核表达载体(pPUR/U6-siJAB1),将其转染RAW264.7细胞后使JAB1的蛋白表达水平显著下降,经LPS刺激后培养上清中炎性因子TNF-α和IL-6的浓度较正常细胞明显降低。    结论    小鼠JAB1基因的低表达使RAW264.7细胞在经LPS诱导后的炎性因子产生降低,推测其在炎症反应中对致炎因素的影响更大
Abstract:
Objective     To study the effect of down-regulation of JAB1 in the inflammatory reaction.       Methods     The recombinant plasmid pPUR/U6-siJAB1 was constructed and transfected into RAW264.7 cell to down-regulate JAB1.The concentrations of TNF-α and IL-6 in the cell culture media were detected by ELISA.       Results     The recombinant plasmid pPUR/U6-siJAB1 was obtained successfully. The expression of JAB1 in RAW264.7 cell was markedly decreased. The concentrations of TNF-α and IL-6 stimulated by LPS in the cell culture media were lower than the normal group significantly.       Conclusion     Low expression of JAB1 in RAW264.7 cell degrades the production of inflammatory cytokines, and JAB1 may exert powerful effect on pro-inflammatory reaction caused by LPS.

参考文献/References:

任泂,粟永萍,李洪涛,等. JAB1表达下调对LPS诱导炎性因子TNF-α和IL-6的影响[J]. 第三军医大学学报,2007, 29(16):1552-1554.

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更新日期/Last Update: 2008-09-11