[1]朱冰,杨建茹,付新平,等.GALV致融性糖蛋白转导肺腺癌细胞的抗肿瘤实验研究[J].陆军军医大学学报(原第三军医大学学报),2007,29(18):1737-1741.
　ZHU Bing,YANG Jian-ru,FU Xin-ping,et al.Antitumor effect of GALV membrane fusion glycoprotein on lung adenocarcinoma in vivo and in vitro[J].J Amry Med Univ (J Third Mil Med Univ),2007,29(18):1737-1741.
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GALV致融性糖蛋白转导肺腺癌细胞的抗肿瘤实验研究(/HTML )
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陆军军医大学学报（原第三军医大学学报）[ISSN:1000-5404/CN:51-1095/R]

卷:
29卷
期数:
2007年第18期
页码:
1737-1741
栏目:
论著
出版日期:
2007-09-30

文章信息/Info

Title:
Antitumor effect of GALV membrane fusion glycoprotein on lung adenocarcinoma in vivo and in vitro
作者:
朱冰; 杨建茹; 付新平; 江跃全
重庆医科大学附属第二医院心胸外科;邯郸市第六医院中心实验室
Author(s):
ZHU Bing;  YANG Jian-ru;  FU Xin-ping;  JIANG Yue-quan
Department of Cardiothoracic Surgery, Second Affiliated Hospital of Chongqing Medical University, Chongqing 400010；Central Laboratory, Sixth Hospital of Handan, Handan 056800, China
关键词:
Ⅰ型嗜肿瘤单纯疱疹病毒; 肺腺癌; 长臂猿白血病病毒致融性外膜糖蛋白
Keywords:
type Ⅰ herpes simplex virus;  lung adenocarcinoma;  Gibbon ape leukemia virus membrane fusion glycoprotein
分类号:
R73-362；R734.2；R730.54
文献标志码:
A
摘要:
目的  探讨特异性启动子控制下嗜肿瘤Ⅰ型单纯疱疹病毒（HSV-Ⅰ）载体介导的长臂猿白血病病毒致融性外膜糖蛋白(GALV.fus)基因转导肺腺癌细胞的抗肿瘤作用。 方法  将重组HSV-Ⅰ载体质粒通过脂质体导入非洲绿猴肾细胞（Vero）中包装制备重组HSV-Ⅰ病毒，用来转染肺腺癌细胞(A549)和正常人胚胎成纤维细胞（HFL-Ⅰ GNHu 5）及裸鼠皮下肺癌转移模型，观察其体外及体内抗肿瘤作用及细胞毒性作用。统计学方法采用t-检验。 结果  成功包装重组HSV-Ⅰ病毒, BALB／c 裸鼠皮下接种A549细胞2周后均有肿瘤生长，成瘤率为100%。重组单纯疱疹病毒能特异性转染A549细胞，治疗组及对照组细胞存活率分别为20%及70%，病毒对A549细胞有明显的杀灭作用且对裸鼠皮下肺癌转移灶抑制明显。  结论  GALV.fus基因对肺癌细胞有强效的抗肿瘤作用，为临床肺癌治疗探索出一种新型基因治疗方法。
Abstract:
Objective    To investigate the antitumor effect of special promoter-controlled Gibbon ape leukemia virus membrane fusion glycoprotein (GALV.fus) mediated by type Ⅰ herpes simplex virus (HSV-Ⅰ) on lung adenocarcinoma.     Methods    Recombinant HSV-Ⅰ plasmids encoding GALV.fus was introduced into green monkey kidney cells（Vero）by liposome to amplify the virus, and then the virus was transfected into lung adenocarcinoma (A549), human fetal fibroblasts (HFL-Ⅰ GNHu 5) and human lung adenocarcinoma xenografts which were established in nude mice subcutaneously to observe antitumor and cytotoxic effect in vitro and in vivo; Recombined cytomegalovirus (CMV) containing GALV.fus or enhanced green fluorescence protein were served as control.     Results    Recombinant HSV-Ⅰ virus were packed successfully. Heterotransplantative tumourigenicity of the tumour was 100% in nude mice after A549 cells were inoculated. Recombinant HSV-Ⅰvirus exerted obvious antitumor effects in vitro, with relative survival rate of 23%, while for CMV virus containing GALV. fus, the rate was 20%, and for CMV virus encoding EGFP, the rate was 68%. Recombinant HSV-Ⅰvirus also showed striking antitumor effect on the implanted tumor.     Conclusion    GALV.fus has powerful effect against lung cancer in vitro and in vivo and maybe a promising candidate for gene therapy.

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更新日期/Last Update: 2008-07-15