[1]郑英如,李力,刘宝英,等.人可溶性增殖诱导配体促HeLa细胞增殖作用的研究[J].第三军医大学学报,2007,29(20):1951-1953.
 ZHENG Ying-ru,LI Li,LIU Bao-ying,et al.Soluble a proliferation-induced ligand promotes proliferation of HeLa cells[J].J Third Mil Med Univ,2007,29(20):1951-1953.
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《第三军医大学学报》[ISSN:1000-5404/CN:51-1095/R]

卷:
29卷
期数:
2007年第20期
页码:
1951-1953
栏目:
论著
出版日期:
2007-10-30

文章信息/Info

Title:
Soluble a proliferation-induced ligand promotes proliferation of HeLa cells
作者:
郑英如李力刘宝英李媛李蓉芬郭建新
第三军医大学:大坪医院野战外科研究所妇产科,基础医学部生物化学与分子生物学教研室
Author(s):
ZHENG Ying-ru LI Li LIU Bao-ying LI Yuan LI Rong-fen GUO Jian-xin
Department of Obstetrics and Gynecology, Institute of Surgery Research, Daping Hospital, Chongqing 400042, Department of Biochemistry and Molecular Biology, College of Medicine, Third Military Medical University, Chongqing 400038, China
关键词:
增殖诱导配体受体宫颈癌细胞增殖
Keywords:
a proliferation-induced ligand (APRIL) receptor uterine cervix cancer cell proliferation
分类号:
R392.11;R737.33
文献标志码:
A
摘要:
目的  观察人可溶性增殖诱导配体(soluble a proliferation-induced ligand, sAPRIL)是否具有促进宫颈癌细胞株HeLa增殖的作用及其机制。  方法  用Brdu法检测sAPRIL蛋白对人宫颈癌HeLa细胞增殖的作用,进而用RT-PCR法检测HeLa细胞APRIL受体的表达情况。  结果  原核表达的sAPRIL蛋白能促进HeLa细胞增殖。HeLa细胞表达APRIL的受体HSPG1、HSPG2,而不表达BCMA 、TACI及HSPG4。  结论  sAPRIL有明确的促HeLa细胞增殖作用。由于HeLa细胞有APRIL受体HSPG1、HSPG2的表达,我们初步推测该作用可能是通过HSPG1及HSPG2起作用的。
Abstract:
Objective    To identify the promoting function of soluble a proliferation-induced ligand (sAPRIL) to the proliferation of HeLa cells and its possible mechanism.     Methods    The proliferation stimulating effect of sAPRIL to HeLa cells was detected by Brdu. RT-PCR was performed to identify the expression of sAPRIL receptors on HeLa cells.     Results    sAPRIL had positive effect to promote the proliferation of HeLa cells. HeLa cells could express sAPRIL receptors HSPG1 and HSPG2 but not BCMA, TACI or HSPG4.     Conclusion    sAPRIL can effectively promote the proliferation of HeLa cells, which might be associated with HSPG1 and HSPG2.

参考文献/References:

郑英如,李力,刘宝英,等.  人可溶性增殖诱导配体促HeLa细胞增殖作用的研究[J].第三军医大学学报,2007,29(20):1951-1953.

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更新日期/Last Update: 2008-07-10