[1]余抒,顾江,曾浩,等.肠出血性大肠埃希菌O157∶H7黏附HeLa细胞模型的建立[J].第三军医大学学报,2007,29(20):1933-1935.
 YU Shu,GU Jiang,ZENG Hao,et al.Establishment of an enterohemorrhagic Escherichia coli O157∶H7 attached HeLa cell model[J].J Third Mil Med Univ,2007,29(20):1933-1935.
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《第三军医大学学报》[ISSN:1000-5404/CN:51-1095/R]

卷:
29卷
期数:
2007年第20期
页码:
1933-1935
栏目:
论著
出版日期:
2007-10-30

文章信息/Info

Title:
Establishment of an enterohemorrhagic Escherichia coli O157∶H7 attached HeLa cell model
作者:
余抒顾江曾浩杨琴刘艳青张卫军罗萍王庆旭毛旭虎
第三军医大学医学检验系临床微生物及免疫学教研室; 成都军区总医院检验科
Author(s):
YU Shu GU Jiang ZENG Hao YANG Qin LIU Yan-qing ZHANG Wei-jun LUO Ping WANG Qing-xu MAO Xu-hu
Department of Clinical Microbiology and Immunology, College of Medical Laboratory Science, Third Military Medical University, Chongqing 400038; Department of Laboratory Medicine, The General Hospital of Chengdu Military Command, Chengdu 610000, China
关键词:
肠出血性大肠埃希菌O157∶H7细胞模型HeLa细胞
Keywords:
Enterohemorrhagic Escherichia coli O157∶H7 cell model HeLa cell
分类号:
R-33;R378.21;R446.5
文献标志码:
A
摘要:
目的  建立肠出血性大肠埃希菌O157∶H7体外黏附HeLa细胞模型,为进一步研究其致病机制奠定基础。 方法  以细菌黏附HeLa细胞数量为指标,分别评价细菌数量、细菌与细胞孵育时间对细菌黏附数量的影响,得到最佳条件,最后肌动蛋白荧光染色(FAS)试验鉴定模型。 结果  加入细菌为1×105 CFU,细菌与细胞孵育4 h时,黏附细胞的数量最佳,FAS检测发现该条件下细菌能够引起细胞特异性黏附、擦拭(attaching & effacing, A/E) 损伤,模型建立成功。 结论  成功建立O157∶H7体外黏附HeLa细胞模型,为进一步研究其致病机制提供了条件。
Abstract:
Objective    To establish Enterohemorrhagic Escherichia coli (EHEC) O157∶H7 attached HeLa cell model for further study on its pathogenic mechanism.     Methods    HeLa cells and Escherichia coli O157∶H7 were incubated together in different condition (amount of bacteria, incubation time). In regard to the amount of bacteria attached, we decided the best condition for O157∶H7 to infect HeLa cells and identified the characterized A/E (attaching and effacing) lesion by FAS (fluorescence actin staining).     Results    The best condition is that 1×105 CFU O157∶H7 and HeLa cells were incubated together for 4 h, under which the specific A/E lesion can be observed.     Conclusion    EHEC O157∶H7 attached HeLa cell model has been successfully established.

参考文献/References:

余抒,顾江,曾浩,等.  肠出血性大肠埃希菌O157∶H7黏附HeLa细胞模型的建立[J].第三军医大学学报,2007,29(20):1933-1935.

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更新日期/Last Update: 2008-07-10