[1]张桂灵,石小枫,冉长清,等.三七总皂甙对抗大鼠免疫性肝纤维化的实验研究[J].第三军医大学学报,2007,29(23):2212-2214.
 ZHANG Gui-ling,SHI Xiao-feng,RAN Chang-qing,et al.Effects of total saponins of panax notoginseng against liver fibrosis in rats[J].J Third Mil Med Univ,2007,29(23):2212-2214.
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三七总皂甙对抗大鼠免疫性肝纤维化的实验研究(/HTML )
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《第三军医大学学报》[ISSN:1000-5404/CN:51-1095/R]

卷:
29卷
期数:
2007年第23期
页码:
2212-2214
栏目:
论著
出版日期:
2007-12-15

文章信息/Info

Title:
Effects of total saponins of panax notoginseng against liver fibrosis in rats
作者:
张桂灵石小枫冉长清徐曼朱照静
浙江省余姚市人民医院感染科;重庆医科大学:附属第二医院感染科,教育部感染性疾病分子生物学重点实验室,基础医学院药学教研室;重庆市中药研究院中药药理室
Author(s):
ZHANG Gui-ling SHI Xiao-feng RAN Chang-qing XU Man ZHU Zhao-jing
Department of Infectious Diseases, Yuyao People’s Hospital; Department of Infectious Diseases, Key Laboratory of Molecular Biology on Infection Diseases, Ministry of Education, The Second Affiliated Hospital, College of Basic Medicine, Chongqing Medical University; Institute of Chongqing Chinese Traditional Medicine
关键词:
三七总皂甙肝纤维化Ⅰ、Ⅲ型胶原TGF-β1
Keywords:
total saponins of panax notoginseng liver fibrosis collagen Ⅰ transforming growth factor β1
分类号:
R-33; R285,R575.2
文献标志码:
A
摘要:
目的  进一步观察三七总皂甙抗大鼠免疫性肝纤维化的作用。  方法  用牛血清白蛋白等复合因素致大鼠肝纤维化,同时给予三七总皂甙60 mg/kg和30 mg/kg皮下注射治疗,共6周。分离血清,检测肝功能;分离肝组织,作病理学纤维化程度分级和免疫组化观察Ⅰ、Ⅲ型胶原及TGF-β1的合成表达。  结果  三七总皂甙能降低血清转氨酶,增加肝脏白蛋白的合成,减轻肝纤维化程度,抑制免疫性肝纤维化大鼠Ⅰ、Ⅲ型胶原及TGF-β1的合成表达。  结论  三七总皂甙具有抗肝纤维化作用。
Abstract:
Objective  To investigate the effect of total saponins of panax notoginseng (PNS) on the production of collagen Ⅰ, Ⅲ and TGF-β1 in rats with experimental fibrosis.   Methods  Experimental fibrosis model was copied by intracutaneous injection of BSA and rats feeding on diet rich in lipid. From the 1st day after BSA injection, SPN (60 or 30 mg/kg) or Colchicine were given intracutaneously once a day for 42 days. All animals were sacrificed on the 43rd day. Their hepatic function was evaluated by determining the levels of ALT, AST, ALB in serum. The progression of hepatic fibrosis was confirmed by pathological analysis. Then type collagenⅠ, Ⅲ and TGF-β1 were observed with immunohistochemical method.   Results  The BSA injection significantly elevated the levels of ALT and AST, while SPN treatment significantly down-regulated them. But the level of ALB was opposite to that of ALT or AST in SPN treatment group. The positive staining of the collagenⅠ, Ⅲ and TGF-β1 was stronger in hepatic fibrosis model group than in SPN treatment group. The contents of the collagen were identical to the immunohistochemical results.   Conclusion  SPN has the protective effect against liver fibrosis.

参考文献/References:

张桂灵,石小枫,冉长清,等.三七总皂甙对抗大鼠免疫性肝纤维化的实验研究[J].第三军医大学学报,2007,29(23):2212-2214.

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更新日期/Last Update: 2008-05-20