[1]陈济,潘巍巍,成海恩,等.细胞周期素E的干扰RNA对HeLa细胞增殖的抑制作用[J].第三军医大学学报,2007,29(22):2135-2138.
 CHEN Ji,PAN Wei-wei,CHENG Hai-en,et al.Inhibition of HeLa cell proliferation by cyclinE RNA interference[J].J Third Mil Med Univ,2007,29(22):2135-2138.
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细胞周期素E的干扰RNA对HeLa细胞增殖的抑制作用(/HTML )
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《第三军医大学学报》[ISSN:1000-5404/CN:51-1095/R]

卷:
29卷
期数:
2007年第22期
页码:
2135-2138
栏目:
论著
出版日期:
2007-11-30

文章信息/Info

Title:
Inhibition of HeLa cell proliferation by cyclinE RNA interference
作者:
陈济潘巍巍成海恩魏丽丽袁成福石华易发平马永平宋方洲
重庆医科大学基础医学院生物化学与分子生物学教研室,临床检验诊断学省部共建教育部重点实验室,重庆市生物化学与分子药理学重点实验室
Author(s):
CHEN Ji PAN Wei-wei CHENG Hai-en WEI Li-li YUAN Cheng-fu SHI Hua YI Fa-ping MA Yong-ping SONG Fang-zhou
Department of Biochemical and Molecular Biology, Faculty of Basic Medical Sciences, Chongqing Medical University, Chongqing 400016, China
关键词:
RNA干扰细胞周期素E宫颈癌HeLa细胞细胞周期
Keywords:
RNA interference cyclin E cervical cancer HeLa cell cell cycle
分类号:
R394-33;R73-362;R737.33
文献标志码:
A
摘要:
目的  利用RNAi技术下调宫颈癌HeLa细胞中cyclin E mRNA水平,研究对HeLa细胞增殖及周期的影响。 方法  构建针对cyclin E基因的RNAi真核表达载体,脂质体转染HeLa细胞下调细胞中cyclin E mRNA含量。RT-PCR检测抑制效果;MTT检测细胞生长情况;流式细胞仪分析HeLa细胞周期变化。 结果  成功构建针对cyclin E基因的RNAi真核表达载体,可以特异下调cyclin E mRNA含量,并抑制细胞恶性增殖。细胞周期分析显示干扰使细胞停留在G0~G1期,S期细胞明显减少。 结论  RNAi能特异地下调HeLa 细胞中cyclin E mRNA含量,抑制宫颈癌细胞恶性增殖,提示cyclin E 可能成为宫颈癌基因治疗中的一个新的靶点。
Abstract:
Objective    To study the effect of RNAi targeting cyclin E on the proliferation and cell cycle of cervical cancer cell line HeLa.     Methods    The eukaryotic expression vector of cyclin E RNAi was constructed and transfected into HeLa cell. The mRNA level of cyclinE was detected by RT-PCR. Cell proliferation was analyzed by MTT;Cell cycle was analyzed by flow cytometry.     Results    The vector of cyclinE RNAi was successfully constructed, and specially knocked down the mRNA level of cyclin E gene after transfection. MTT showed the cell proliferation was inhibited. Cells stopped at G0-G1 phase and cell number in S phase was sharply decreased after RNAi.     Conclusion    The mRNA level of cyclinE gene in HeLa cell can be specially knocked down by RNAi, and the malignant proliferation of cervical cancer cells is inhibited too.

参考文献/References:

陈济,潘巍巍,成海恩,等.  细胞周期素E的干扰RNA对HeLa细胞增殖的抑制作用[J]. 第三军医大学学报, 2007, 29(22):2135-2138.

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更新日期/Last Update: 2008-07-01