<sup>18</sup>F-FMISO在肺癌模型小鼠体内的生物分布及PET显像研究-《第三军医大学学报》

文章信息/Info

Title:: Biodistribution and PET imaging of ¹⁸F-fluoromisonidazole in murine model of lung carcinoma

Author(s):: WANG Yu-qi; ZHENG Xin; ZHOU Nai-kang; ZHANG Jin-ming; LIU Xi; ZHANG Jing; Division of General Thoracic Surgery, Department of Nuclear Medicine, General Hospital of PLA, Beijing 100853, China

Keywords:: ¹⁸F-fluoromisonidazole; hypoxic imaging contract; biodistribution; pulmonary neoplasms; animal model

摘要:: 目的评价乏氧显像剂¹⁸F-硝基咪唑丙醇（¹⁸F-FMISO）在肺腺癌动物模型中的诊断价值。方法荷肺腺癌T739小鼠经尾静脉注入¹⁸F-FMISO后不同时间（30、60、90、120 min）处死，测量¹⁸F-FMISO的生物分布，并行PET显像。对照组小鼠在注入¹⁸F-氟脱氧葡萄糖（¹⁸F-FDG）后行PET显像。结果荷瘤鼠肿瘤PET显像清晰。生物分布研究表明肿瘤有明显的放射性摄取。在肾脏和肝脏有较高的放射性分布。肿瘤对血和肌肉的T/NT比值均大于2。结论肺部恶性肿瘤组织中¹⁸F-FMISO摄取高于正常组织，可通过PET清晰显像，为进一步临床应用于肿瘤的乏氧诊断提供了依据。

Abstract:: Objective To evaluate the diagnosis value of a hypoxic contrast, ¹⁸F-fluoromisonidazole (¹⁸F-FMISO), in murine model of pulmonary carcinoma. Methods After the injection of ¹⁸F-FMISO into the tail, 48 T739 mice bearing the pulmonary adenocarcinoma were sacrificed and divided into 4 groups according different killing times (30, 60, 90 and 120 min). The biodistribution was measured and PET scanning was performed. Another 8 tumor bearing T734 mice served as control, and PET was carried out after the injection of ¹⁸F-FDG. Results The tumor PET images with ¹⁸F-FMISO were clear. Considerable radioactive uptake on tumor was observed. Higher radioactivity was found in the kidneys and liver. The T/NT ratios of tumor/muscle and tumor/blood were above 2.0. Conclusion The uptake of ¹⁸F-FMISO in pulmonary malignant tissues is higher than that in normal tissues, thus the pulmonary neoplasm can be identified accurately with PET imaging. Our preliminary study provides a basis for further clinical hypoxic diagnose of the tumor.