[1]邓均,艾国平,周桃莉,等.G-CSF动员循环间充质干细胞及其对小鼠颅脑损伤修复的可行性分析[J].第三军医大学学报,2007,29(12):1131-1134.
 DENG Jun,AI Guo-ping,ZHOU Tao-li,et al.Feasibility of MSCs mobilization by G-CSF and its prosthetic effect in traumatic brain injury[J].J Third Mil Med Univ,2007,29(12):1131-1134.
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G-CSF动员循环间充质干细胞及其对小鼠颅脑损伤修复的可行性分析(/HTML )
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《第三军医大学学报》[ISSN:1000-5404/CN:51-1095/R]

卷:
29卷
期数:
2007年第12期
页码:
1131-1134
栏目:
论著
出版日期:
2007-06-30

文章信息/Info

Title:
Feasibility of MSCs mobilization by G-CSF and its prosthetic effect in traumatic brain injury
作者:
邓均艾国平周桃莉王军平徐辉邹仲敏董世武郝磊冉新泽粟永萍
第三军医大学:军事预防医学院防原医学教研室,全军复合伤研究所,创伤、烧伤与复合伤国家重点实验室,基础医学部病原生物学教研室
Author(s):
DENG Jun AI Guo-ping ZHOU Tao-li WANG Jun-ping XU Hui ZOU Zhong-min DONG Shi-wu HAO Lei RAN Xin-ze SU Yong-ping
State Key Laboratory of Trauma, Burns and Combined Injury, Institute of Combined Injury, College of Preventive Medicine,Department of Pathobiology, College of Medicine, Third Military Medical University, Chongqing 400038, China
关键词:
间充质干细胞创伤动员G-CSF
Keywords:
mesenchymal stem cell trauma mobilization G-CSF
分类号:
R329.21;R331.22;R651.15
文献标志码:
A
摘要:
目的    建立小鼠严重颅脑创伤模型探讨粒细胞集落刺激因子(granulocyte colony-stimulating factor, G-CSF)动员循环间充质干细胞(mesenchymal stem cells, MSCs)的可行性,并观察G-CSF动员的修复效应。    方法    以G-CSF作为动员剂,培养骨髓和外周血来源的MSCs,计数动员前后的CFU-F。制作小鼠严重颅脑创伤模型,致伤后采用G-CSF对MSCs进行动员,在2、24、48、96、120、144、192、264、336 h 进行神经行为学评分、运动功能评分,并观察小鼠死亡率和病理组织切片。    结果    从小鼠外周血培养出MSCs,G-CSF动员后骨髓和外周血MSCs的CFU-F数量显著高于正常对照组(P<0.01)。致伤后小鼠神经行为学和运动功能评分显著低于正常对照组(P<0.01),创伤治疗组在144~192 h期间评分显著高于创伤组(P<0.05)。致伤后小鼠死亡率增高,病理切片可见创伤处有出血和空泡。    结论    成功培养及用G-CSF动员了外周血MSCs,在成功建立小鼠严重颅脑创伤模型的基础上,显示G-CSF动员可以降低严重颅脑创伤小鼠死亡,参与了创伤修复。
Abstract:
Objective    To explore the feasibility of mobilization circulating MSCs by G-CSF and observe the repairing effect of G-CSF mobilization in severe mouse traumatic brain injury (TBI) model.     Methods    MSCs-derived bone marrow and peripheral blood (PB) were cultured and its CFU-F were counted after mobilization by G-CSF. At 2, 24, 48, 96, 120, 144, 192, 264, 336 h after severe TBI in mice was establish, the neurobehavior of mice was measured by neurological examination and motor functional test, and mortality rate and pathologic changes were analyzed.     Results    MSCs-derived PB were successfully cultured. The CFU-F of mobilization group increased significantly than that of control group (P<0.01). After TBI, the neurobehavioral and motor function scores decreased significantly as compared with that of normal group (P<0.01), the scores of treatment group increased significantly as compared with that of trauma group (P<0.05). The mortality rate was high after TBI, and brain injury such as haemorrhage and vacuolus were confirmed by pathologic examinations.     Conclusion    G-CSF can mobilize MSCs-derived PB. G-CSF mobilization can decrease the death of TBI mice and participate repair in trauma of TBI mice.

参考文献/References:

邓均,艾国平,周桃莉,等. G-CSF动员循环间充质干细胞及其对小鼠颅脑损伤修复的可行性分析[J]. 第三军医大学学报, 2007, 29(12):1131-1134.

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更新日期/Last Update: 2008-10-23