[1]刘耀,张曦,陈幸华,等.急性白血病化疗前后与正常人骨髓基质细胞间通讯的改变[J].第三军医大学学报,2007,29(10):879-881.
 LIU Yao,ZHANG Xi,CHEN Xing-hua,et al.Intercellular communicating function in BMSCs of acute leukemia before and after chemotherapy and normal BMSCs[J].J Third Mil Med Univ,2007,29(10):879-881.
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《第三军医大学学报》[ISSN:1000-5404/CN:51-1095/R]

卷:
29卷
期数:
2007年第10期
页码:
879-881
栏目:
论著
出版日期:
2007-05-30

文章信息/Info

Title:
Intercellular communicating function in  BMSCs of acute leukemia before and after chemotherapy and normal BMSCs
作者:
刘耀张曦陈幸华李忠俊司英健高蕾
第三军医大学新桥医院血液内科
Author(s):
LIU Yao ZHANG Xi CHEN Xing-hua LI Zhong-jun SI Ying-jian GAO Lei
Department of Hematology, Xinqiao Hospital, Third Military Medical University, Chongqing 400037, China
关键词:
急性白血病骨髓基质细胞Connexin43GJIC化疗
Keywords:
acute leukemia bone marrow stromal cell Connexin43 gap junction intercellular communication chemotherapy
分类号:
R329.25;R331.22;R733.705
文献标志码:
A
摘要:
目的    观察急性白血病化疗前后与正常人骨髓基质细胞间隙连接蛋白43 (connexin43, Cx43)表达的变化及通讯功能的改变。    方法    体外培养初发急性白血病化疗前及化疗后完全缓解的与正常人的骨髓基质细胞,采用免疫细胞化学方法观察三者之间Cx43表达的变化;采用细胞划痕染料传输技术比较三者之间间隙连接细胞间通讯(gap junction intercellular communication, GJIC)功能的差异。    结果    正常人、急性白血病化疗前及化疗后完全缓解的骨髓基质细胞上Cx43表达的阳性率分别为(88.0±3.5)%、(12.0±2.4) %和(52.0±3.1) %;基质细胞上Cx43蛋白的光密度值分别为(175.08±8.34)、(45.42±3.71)及(94.33±7.20);染料传输的细胞数分别为(9.20±0.35)、(1.84±0.33)和(4.07±0.53)。    结论    急性白血病骨髓基质细胞间通讯功能较正常人及化疗后完全缓解急性白血病骨髓基质细胞明显减弱。
Abstract:
Objective    To observe the connexin protein 43 (Cx43) expression levels in  BMSCs of acute leukemia before and after chemotherapy and normal BMSCs and study the communication function between cells.     Methods    In in vitro cultured  BMSCs of acute leukemia before and after chemotherapy and normal BMSCs, the Cx43 expression levels were detected by immunochemistry and the difference of gap junction intercellular communication (GJIC) were examined by using dye transfer technique.     Results    In the BMSCs of acute leukemia before and after chemotherapy and normal BMSCs, the positive rate of Cx43 expression was (88.0±3.5)%, (12.0±2.4)% and (52.0±3.1)% respectively, average optical density value of stromal cells were (175.08±8.34), (45.42±3.71) and (94.33±7.20) and the cell number through dye transmission of stromal cells were (9.20±0.35), (1.84±0.33) and (4.07±0.53).     Conclusion    Cell-cell communication function of BMSCs of acute leukemia before chemotherapy is significantly lower than that of normal BMSCs and BMSCs of acute leukemia after chemotherapy.

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更新日期/Last Update: 2008-10-24