[1]邹显巍,吴珊.大鼠脑出血后脑肺组织NF-κB的表达及其在急性肺损伤中的作用[J].第三军医大学学报,2007,29(19):1859-1862.
 ZOU Xian-wei,WU Shan.Expression of NF-κB in the brain and lung and its role in acute lung injury following intracerebral hemorrhage of rats[J].J Third Mil Med Univ,2007,29(19):1859-1862.
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大鼠脑出血后脑肺组织NF-κB的表达及其在急性肺损伤中的作用(/HTML )
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《第三军医大学学报》[ISSN:1000-5404/CN:51-1095/R]

卷:
29卷
期数:
2007年第19期
页码:
1859-1862
栏目:
论著
出版日期:
2007-10-15

文章信息/Info

Title:
Expression of NF-κB in the brain and lung and its role in acute lung injury following intracerebral hemorrhage of rats
作者:
邹显巍吴珊
贵阳医学院附属医院神经内科
Author(s):
ZOU Xian-wei WU Shan
Department of Neurology, Affiliated Hospital of Guiyang Medical College, Guiyang 550004, China
关键词:
NF-кB脑出血急性肺损伤
Keywords:
nuclear factor-kappa Bintracerebral hemorrhageacute lung injury
分类号:
R563.9;R741.02; R743.34
文献标志码:
A
摘要:
目的  探讨NF-κB在脑出血(intracerebral hemorrhage,ICH)后在脑、肺组织的表达及在急性肺损伤(acute lung injury ,ALI)中的作用。  方法  采用自体血尾状核注入法制备脑出血大鼠模型。将35只大鼠随机分为假手术组(5只),脑出血组(30只),脑出血组又分为6 、12、 24 、48 h和3、5 d 6个亚组。应用HE染色观察各组脑出血周围组织与肺组织的炎症反应情况。RT-PCR法检测脑和肺组织中NF-κB p65 mRNA的表达。放射免疫法测定血清中TNF-α和IL-1β的含量。  结果  脑出血后12 h脑水肿明显,24 h血肿周围炎症细胞浸润增多,48 h左右炎症反应最重;脑出血6 h即有间质性肺炎表现和局灶性肺气肿,48~72 h炎症反应达高峰,肺泡结构破坏明显;与假手术组相比,NF-κB p65 mRNA于脑出血6 h在脑血肿周围组织与肺组织中表达均开始增高(P<0.01),48 h均达高峰(P<0.01),3~5 d表达仍高于假手术组(P<0.01);血清TNF-α含量在脑出血6 h开始增高(P<0.01),48 h最高(P<0.01),5 d时仍高于假手术组(P<0.01);IL-1β在脑出血各组中含量均无增高。  结论  脑出血可以通过引发炎症反应导致急性肺损伤,NF-кB在其病理生理学过程中起重要作用。
Abstract:
Objective    To investigate the role of NF-κB in acute lung injury following intracerebral hemorrhage.     Methods    Using stereotactic apparatus we infused autologous blood into the caudate nucleus to build up rat  ICH model. A total of 35 male adult Wistar rats were randomly divided into sham-operation group(n=5) and hemorrhage group(n=30) that was subsequently divided into 6 subgroups (6 h, 12 h, 24 h, 48 h, 3 d, and 5 d, n=5). The inflammatory reaction degrees in the peripheral tissues of hematoma in the brain and lung were observed after HE staining. The levels of NF-κB p65 mRNA in the brain and lung were assayed using RT-PCR. The content of TNF-α and IL-1β in serum was determined by radioimmunoassay (RIA) method.     Results    Brain edema was obvious at 12 h after intracerebral hemorrhage and the inflammatory cells infiltration increased at 24 h and reached maximum at 48 h in the peripheral tissues of hematoma. Interstitial pneumonia and local emphysema appeared at 6 h and got to peak at 48-72 h, in which alveolar structure was significantly destroyed. In comparison with sham-operation group, the expression of NF-κB p65 mRNA began to increase at 6 h in brain and lung tissues (P<0.01), and reached maximum at 48 h (P<0.01). Thereafter it began decreasing but was still higher at 5 d (P<0.01). The content of serum TNF-α began to increase at 6 h,got to peak at 48 h, and still higher than the sham-operation group at 5 d (P<0.01). The content of IL-1β did not increase among all intracerebral hemorrhage groups.     Conclusion    Intracerebral hemorrhage can induce acute lung injury through irritating the cascade of inflammation. NF-κB plays a critical role in the pathophysiology of inflammation.

参考文献/References:

邹显巍,吴珊. 大鼠脑出血后脑肺组织NF-κB的表达及其在急性肺损伤中的作用[J].第三军医大学学报,2007,29(19):1859-1862.

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更新日期/Last Update: 2008-07-15