[1]赵玲,杜晓兰,苏楠,等.HIF-1α条件性基因敲除嵌合体小鼠的获得[J].第三军医大学学报,2007,29(14):1361-1363.
 ZHAO Ling,DU Xiao-lan,SU Nan,et al.Generation of chimeras for HIF-1α conditional knockout mice[J].J Third Mil Med Univ,2007,29(14):1361-1363.
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HIF-1α条件性基因敲除嵌合体小鼠的获得(/HTML )
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《第三军医大学学报》[ISSN:1000-5404/CN:51-1095/R]

卷:
29卷
期数:
2007年第14期
页码:
1361-1363
栏目:
论著
出版日期:
2007-07-30

文章信息/Info

Title:
Generation of chimeras for HIF-1α conditional knockout mice
作者:
赵玲杜晓兰苏楠宋瑞华何启芬李福兵戚华兵陈林
第三军医大学大坪医院野战外科研究所全军创伤中心,创伤、烧伤与复合伤国家重点实验室
Author(s):
ZHAO Ling DU Xiao-lan SU Nan SONG Rui-hua HE Qi-feng LI Fu-bing QI Hua-bing CHEN Lin
State Key Laboratory of Trauma, Burns and Combined Injury, Trauma Center, Institute of Surgery Research, Daping Hospital, Third Military Medical University, Chongqing 400042, China
关键词:
低氧诱导因子-1α条件性基因敲除同源重组胚胎干细胞Cre/loxP系统
Keywords:
hypoxia inducible factor-1α conditional knockout homologous recombination embryonic stem cell Cre/loxP system
分类号:
R-332;R394-33;R394.2
文献标志码:
A
摘要:
目的    应用Cre/loxP系统建立HIF-1α条件性基因敲除小鼠模型。    方法    电击法将构建好的HIF-1α条件性基因敲除载体转染入小鼠ES细胞,用G418与Ganciclovir对转染的ES细胞进行正负筛选,经Southern杂交鉴定得到阳性ES细胞克隆后,将已发生正确重组的ES细胞显微注射入小鼠囊胚腔,对子代鼠进行基因型鉴定。    结果    经G418与Ganciclovir筛选培养得到490个阳性克隆,用5′端探针进行Southern杂交鉴定获得1株发生同源重组的ES细胞克隆,经显微注射,得到5只由基因敲除ES细胞和供体囊胚细胞共同发育而来的嵌合体小鼠。    结论    成功得到基于Cre/loxP系统的HIF-1α条件性基因敲除嵌合体小鼠模型。
Abstract:
Objective    To generate chimeras for hypoxia inducible factor-1α (HIF-1α) conditional knockout mice using Cre/loxP system.     Methods    The mouse ES cells were electroporated with conditional knockout vector of HIF-1α, selected by G418-positive and Ganciclovir-negative culture. Target ES cells, following positive and negative selection, was identified by Southern blotting. Chimera mice were born by pseudopregenant mice which were transplanted with blastocysts microinjected with target ES cells.     Results    A total of 490 ES clones were collected after screening by both G418-positive and Ganciclovir-negative selection, and one clone with correct homologous recombinant event was obtained by Southern blotting screening. Five chimeras were produced following blastocysts microinjection and transplantation.     Conclusion    Chimera mice with HIF-1α conditional knockout allele were obtained, which will help to finally obtain HIF-1α conditional knockout mice, subsequently provide the possibility to study HIF-1α function in vivo.

参考文献/References:

赵玲,杜晓兰,苏楠,等. HIF-1α条件性基因敲除嵌合体小鼠的获得[J]. 第三军医大学学报, 2007, 29(14):1361-1363.

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更新日期/Last Update: 2008-10-07