[1]王炼炼,丘彦,幸贵邦.表皮生长因子促进小鼠未成熟卵母细胞体外成熟的实验研究[J].第三军医大学学报,2007,29(22):2179-2181.
 WANG Lian-lian,QIU Yan,XING Gui-bang.Epidermal growth factor improves in vitro maturation of immature mouse oocytes[J].J Third Mil Med Univ,2007,29(22):2179-2181.
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《第三军医大学学报》[ISSN:1000-5404/CN:51-1095/R]

卷:
29卷
期数:
2007年第22期
页码:
2179-2181
栏目:
论著
出版日期:
2007-11-30

文章信息/Info

Title:
Epidermal growth factor improves in vitro maturation of immature mouse oocytes
作者:
王炼炼丘彦幸贵邦
重庆医科大学附属第一医院辅助生殖中心
Author(s):
WANG Lian-lianQIU Yan XING Gui-bang
Center of Assisted Reproduction,First Affiliated Hospital of Chongqing Medical University, Chongqing 400016, China
关键词:
表皮生长因子卵母细胞体外成熟
Keywords:
epidermal growth factor oocyte in vitro maturation
分类号:
R-33;R321.1;R329.2
文献标志码:
A
摘要:
目的  了解表皮生长因子(epidermal growth factor,EGF)对小鼠未成熟卵母细胞体外成熟、受精及胚胎发育的影响。  方法  将生殖泡(germinal vesicle,GV)期卵母细胞分别在EGF浓度为0(对照)、0.5、1、2.5、5、10 ng/ml的培养液中进行体外培养,观察生殖泡破裂(germinal vesicle breakdown, GVBD)和第一极体(the first polar body,PB1)排出的情况。将达到MⅡ期的卵母细胞进行体外受精,观察原核和胚胎发育情况。  结果  EGF浓度为1、2.5、5、10 ng/ml组GVBD发生率高于对照组;EGF浓度2.5、5、10 ng/ml组PB1排出率高于对照组,其中EGF浓度5 ng/ml组的GVBD、PB1发生率最高(P<0.01)。卵母细胞在1、2.5、5、10 ng/ml EGF培养浓度下的退化率低于对照组(P<0.05,P<0.01)。卵母细胞的体外受精率、2-细胞和4-细胞胚胎的发生率在EGF浓度为5 ng/ml时明显高于对照组(P<0.01)。  结论  EGF可促进GV期卵母细胞的成熟,对其受精能力和胚胎发展也有促进作用,也可能有抑制卵母细胞凋亡的作用,而EGF以上作用与剂量有关。
Abstract:
Objective    To investigate the significance of EGF in the in vitro maturation, fertilization, and pre-implantation development of the immature oocytes.     Methods    The immature oocytes were isolated from the ovaries of the rats. The germinal vesicle (GV) oocytes were cultured in the EGF solutions at concentrations of 0(control), 0.5, 1, 2.5, 5 and 10 ng/ml Ham’s F10 culture media. The status of germinal vesicle break-down(GVBD) and the extrusion of the first polar body (PB1) were observed. The achieved MⅡoocytes were then inseminated in vitro; and the pronuclei (PN) and the pre-implantaon development were observed. The gradeⅠ-Ⅱ4-cell embryos were selected and implanted into the fallopian tube of the rats with the 1 day pseudogestation. Follow-up observations were made till the delivery.     Results    After GV oocytes were treated with EGF solutions at concentration of 1, 2.5, 5 and 10 ng/ml for 12, 16, 20, and 24 h, the  GVBD in the GV cumulus-oocyte complexs(COC) have better outcomes than that of the control group(P<0.05, P<0.01). For 20, 24, and 36 h in the 2.5, 5 and 10 ng/ml EGF groups, the elimination ratio of PB1 was higher than that of the control group(P<0.05, P<0.01), particularly in the 5 ng/ml EGF (P<0.01). The degenerating rates of the COC group cultured in the 1, 2.5, 5 and 10 ng/ml EGF were lower than that of the control group(P<0.05, P<0.01). The fertilization rates of the oocytes and the incidence rate of 2-cells and 4-cells in the 5 ng/ml EGF were higher than those of the control group (P<0.01).     Conclusion    EGF promotes not only the maturation of COC in the GV phase, but also the fertilization and pre-implantation development in a dose-dependent fashion. enhancing effect of EGF was closely related to the dosage of EGF.

参考文献/References:

王炼炼,丘彦,幸贵邦.  表皮生长因子促进小鼠未成熟卵母细胞体外成熟的实验研究[J]. 第三军医大学学报, 2007, 29(22):2179-2181.

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更新日期/Last Update: 2008-07-01