[1]陈建明,范士志,蒋耀光,等.二氮嗪与1, 6-二磷酸果糖心麻痹液对大鼠心肌细胞的保护作用[J].第三军医大学学报,2007,29(09):786-788.
 CHEN Jian-ming,FAN Shi-zhi,JIANG Yao-guang,et al.Effects of cardioplegic solution supplemented with diazoxide and fructose-1,6- diphosphate on myocardial apoptosis of cryopreserved rat hearts[J].J Third Mil Med Univ,2007,29(09):786-788.
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二氮嗪与1, 6-二磷酸果糖心麻痹液对大鼠心肌细胞的保护作用
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《第三军医大学学报》[ISSN:1000-5404/CN:51-1095/R]

卷:
29卷
期数:
2007年第09期
页码:
786-788
栏目:
论著
出版日期:
2007-05-15

文章信息/Info

Title:
Effects of cardioplegic solution supplemented with diazoxide and fructose-1,6- diphosphate on myocardial apoptosis of cryopreserved rat hearts
作者:
陈建明范士志蒋耀光王如文何勇李坤
第三军医大学大坪医院野战外科研究所全军胸外科中心
Author(s):
CHEN Jian-ming FAN Shi-zhi JIANG Yao-guang WANG Ru-wen HE Yong LI Kun
Thoracic and  Cardiovascular Surgery Research Center, Institute of Surgery Research, Daping Hospital, Third Military Medical University, Chongqing 400042, China
关键词:
二氮嗪16-二磷酸果糖心脏保存心肌细胞凋亡
Keywords:
diazoxide fructose-16-diphosphate heart preservation myocardial apoptosis
分类号:
R322.11; R617; R96
文献标志码:
A
摘要:
目的      观察含二氮嗪(DZ)与1,6-二磷酸果糖(FDP)心麻痹液对冷保存大鼠心脏心肌细胞凋亡的影响。      方法      SD大鼠24只,分为3组(n=8):对照组、STH组和DF组。离体心脏在4 ℃条件下保存6 h后,在Langendorff装置灌注20 min,转为离体左心脏工作模型10 min。实验终了取心肌组织,检测心肌细胞凋亡指数;心肌丙二醛(MDA)含量,心肌线粒体钙离子含量([Ca2+]m)、细胞质细胞色素C(Cyt c)含量、细胞质caspase-3活性;部分心肌液氮保存,采用RT-PCR检测心肌细胞caspase-3 mRNA含量。      结果      与对照组比较,STH组与DF组再灌注后心肌细胞凋亡指数较明显升高(P<0.01),但DF组明显低于STH组(P<0.01)。STH组心肌MDA含量、[Ca2+]m、细胞质 Cyt c 含量显著增高,细胞质caspase-3 mRNA表达增加,caspase-3活性明显高于对照组(P<0.01)。DF组较STH组各项指标显著改善(P<0.01)。      结论      含二氮嗪与1,6-二磷酸果糖心麻痹液能减少缺血再灌注引起的心肌细胞凋亡。
Abstract:
Objective      To investigate the effects of St. Thomas Ⅱ solution supplemented with diazoxide (DZ) and fructose-1,6-diphosphate (FDP) on myocardial apoptosis in cryopreserved rat hearts.        Methods      Twenty-four SD rats were randomly divided into 3 groups: control, STH and DF. The isolated hearts were stored at 4 ℃ for 6 h except control group, STH group in St. ThomasⅡ solution and DF group in St. Thomas Ⅱ solution supplemented with DZ and FDP. All hearts were perfused by Langendorff model for 20 min, then changed into isolated working heart model for 10 min. The myocardial cell apoptosis was determined with terminal deoxynucleotidyl transferase-mediated nick end labeling (TUNEL) method. The content of cytosolic malondialdehyde (MDA), the mitochondrial Ca2+ concentration ([Ca2+]m) were detected and the content of cytosolic Cytochrome c (Cyt c) protein by Western blotting was evaluated. The expression of caspase-3 mRNA was evaluated by RT-PCR. The activity of cytosolic caspase-3 was determined with fluorescent assay.        Results      Compared with controls, the levels of apoptotic index, caspase-3 activity and its mRNA content, [Ca2+]m, the content of cytosolic MDA and Cyt c protein were significantly increased in STH group. These parameters of DF group were lower significantly than STH group (P<0.01).        Conclusion      Cardioplegic solution supplemented with DZ and FDP could restrain apoptosis of myocardial cells caused by ischemical reperfusion injury.

参考文献/References:

陈建明, 范士志, 蒋耀光, 等. 二氮嗪与1, 6-二磷酸果糖心麻痹液对大鼠心肌细胞的保护作用[J]. 第三军医大学学报, 2007, 29(9):786-788.

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更新日期/Last Update: 2008-10-24