[1]李琴山,刘洋,冯赞杰,等.单核细胞趋化蛋白-1对人脐静脉内皮细胞凋亡的影响[J].第三军医大学学报,2007,29(17):1682-1684.
 LI Qin-shan,LIU Yang,FENG Zan-jie,et al.Monocyte chemotactic protein-1 induces the apoptosis of human umbilical vein endothelial cells[J].J Third Mil Med Univ,2007,29(17):1682-1684.
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《第三军医大学学报》[ISSN:1000-5404/CN:51-1095/R]

卷:
29卷
期数:
2007年第17期
页码:
1682-1684
栏目:
论著
出版日期:
2007-09-15

文章信息/Info

Title:
Monocyte chemotactic protein-1 induces the apoptosis of human umbilical vein endothelial cells
作者:
李琴山刘洋冯赞杰张亚玲钱民章
遵义医学院基础部生物化学教研室
Author(s):
LI Qin-shan LIU Yang FENG Zan-jie ZHANG Ya-ling QIAN Min-zhang
Department of Biochemistry, Zunyi Medical College , Zunyi 563003, China
关键词:
单核细胞趋化蛋白-1人脐静脉内皮细胞凋亡动脉粥样硬化
Keywords:
monocyte chemotactic protein-1 human umbilical vein endothelial cell apoptosis atherosclerosis
分类号:
R329.26; R322.12; R344.81
文献标志码:
A
摘要:
目的  研究单核细胞趋化蛋白-1(monocyte chemotactic protein-1, MCP-1)对人脐静脉内皮细胞(human umbilical vein endothelial cells, hUVEC)凋亡的影响。  方法  培养并鉴定人脐静脉内皮细胞;用不同浓度MCP-1(0.1、1.0、10、100 μg/L)对其作用24、48 h;先用MTT比色法观察细胞存活率、流式细胞技术检测细胞DNA含量及细胞周期观察其对hUVEC凋亡的影响。  结果  单核细胞趋化蛋白-1能诱导人脐静脉内皮细胞的凋亡(P<0.05),其效应随浓度和时间的增加而增强:MTT明显减低;细胞DNA含量减少,出现明显亚G0/G1峰(凋亡细胞峰)。  结论  单核细胞趋化蛋白-1能诱导人脐静脉内皮细胞凋亡。
Abstract:
Objective    To study the effects of monocyte chemotactic protein-1(MCP-1) on the growth of  human umbilical vein endothelial cells (hUVEC).     Methods    hUVEC were cultured in vitro and identified. hUVEC that grew vigorously were stimulated for 24 h or 48 h respectively with MCP-1(0.1, 1.0, 10, and 100 μg/L). The survival rate of hUVEC was first detected by MTT assay. The cell cycle and DNA content were detected and analyzed by flow cytometry.     Results    hUVEC were isolated from human umbilical veins and cultured, and then identified by immunofluorescence and immunohistochemistry with factor Ⅷ and KDR. MCP-1 induced the apoptosis of hUVEC in a dose-dependentmanner and a time-dependentmanner (P<0.05). Compared with control group, MTT and chromosomal DNA content decreased significantly.     Conclusion    MCP-1 can induce the apoptosis of hUVEC.

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更新日期/Last Update: 2008-07-21