[1]田坤,钱桂生.人CD40L真核表达体系的建立[J].第三军医大学学报,2007,29(10):963-966.
 TIAN Kun,QIAN Gui-sheng.Construction of eukaryotic expression system of a cell surface molecule——human CD40 ligand[J].J Third Mil Med Univ,2007,29(10):963-966.
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《第三军医大学学报》[ISSN:1000-5404/CN:51-1095/R]

卷:
29卷
期数:
2007年第10期
页码:
963-966
栏目:
论著
出版日期:
2007-05-30

文章信息/Info

Title:
Construction of eukaryotic expression system of a cell surface molecule——human CD40 ligand
作者:
田坤 钱桂生
第三军医大学新桥医院全军呼吸内科研究所,全军呼吸病研究重点实验室
Author(s):
TIAN Kun QIAN Gui-sheng
Institute of Respiratory Diseases, Xinqiao Hospital, Third Military Medical University, Chongqing 400037, China
关键词:
人CD40L真核表达载体肿小细胞肺癌细胞
Keywords:
human CD40L eukaryotic expression vector SCCL cell line
分类号:
R392-33; R394.2; R730.51
文献标志码:
A
摘要:
目的    构建人CD40L真核表达体系并鉴定其在转染细胞中的表达。    方法    以DNA重组技术构建CD40L的真核表达载体pcDNA3.1(+)-CD40L,脂质体法转染于人小细胞肺癌细胞株h446,流式细胞技术鉴定其在转染细胞中的表达。    结果    双酶切及DNA测序证明,CD40L DNA插入表达载体pcDNA3.1(+) 序列及方向正确,转染肺小细胞肺癌细胞株h446后,经流式细胞仪鉴定转染细胞中存在CD40L表达。    结论    成功地建立人CD40L表达体系,为构建以人CD40L为组件的抗肿瘤疫苗的临床研究奠定基础。
Abstract:
Objective    To construct human eukaryotic expression system expressing T cell surface molecule CD40L and identify the expression product in SCCL cell line h446.     Methods    The expression vector pcDNA3.1-CD40L was constructed by recombinant DNA technology, then transfected into h446 cell line of SCCL by lipidosome methods, and the expressed membrane protein was identified by flow cytometry.     Results    The pcDNA3.1(+)-CD40L was constructed successfully and the coding region was inserted into the vector correctly. The membrane protein was observed by flow cytometry on transfected h446 cell line of SCCL.     Conclusion    Human CD40L was expressed successfully in eukaryotic system, which is very important in the anti-tumor vaccination study with a recombinant human CD40L in clinic.

参考文献/References:

田坤, 钱桂生. 人CD40L真核表达体系的建立[J]. 第三军医大学学报, 2007, 29(10):963-966.

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更新日期/Last Update: 2008-10-24