[1]崔洁,曹参祥,郑静,等.脑出血大鼠脑组织MDA、TNF-α含量变化及其依达拉奉干预效应的研究[J].第三军医大学学报,2007,29(11):1032-1035.
　CUI Jie,CAO Can-xiang,ZHENG Jing,et al.Changes of TNF-alpha and malondialdehyde content in brain tissue and therapeutic effect of edaravone on rats after intracerebral hemorrhage[J].J Third Mil Med Univ,2007,29(11):1032-1035.
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脑出血大鼠脑组织MDA、TNF-α含量变化及其依达拉奉干预效应的研究
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《第三军医大学学报》[ISSN:1000-5404/CN:51-1095/R]

卷:
29卷
期数:
2007年第11期
页码:
1032-1035
栏目:
论著
出版日期:
2007-06-15

文章信息/Info

Title:
Changes of TNF-alpha and malondialdehyde content in brain tissue and therapeutic effect of edaravone on rats after intracerebral hemorrhage
作者:
崔洁; 曹参祥; 郑静; 王景周
第三军医大学大坪医院野战外科研究所神经内科；河北省保定市第二医院南院神经内科
Author(s):
CUI Jie;  CAO Can-xiang;  ZHENG Jing;  WANG Jing-zhou
Department of Neurology, Institute of  Surgery Research, Daping Hospital, Third Military Medical University, Chongqing 400042, Department of Neurology, South Branch of Baoding Second Hospital, Baoding 071051, China
关键词:
依达拉奉; 脑出血; 脑含水量; 丙二醛;  ;  肿瘤坏死因子-α
Keywords:
edaraven;  intracerebral hemorrhage;  brain water content;  malondialdehyde;  tumor necrosis factor-alpha
分类号:
R329.25; R743.34; R971
文献标志码:
A
摘要:
目的    观察3-甲基-1-苯基-2-吡唑啉-5-酮（MCI-186,商品名为依达拉奉）对出血性脑组织的保护作用和可能机制。    方法    将96只Sprague-Dawley大鼠按随机数字表法分为假手术组、脑出血模型组、依达拉奉治疗组，每组32只。采用自体血脑内注入法制作大鼠脑出血模型，假手术组只进针不注血。依达拉奉治疗组大鼠腹腔注射依达拉奉（3 mg/kg），分别于造模前30 min及造模后1次/12 h。于6、24、72、168 h分别用Longa标准测定神经功能缺损；干、湿重法检测脑含水量；酶联免疫吸附法（ELISA)和硫代巴比妥酸法测定血肿周围组织中脑组织TNF-α和丙二醛(malondialdehyde,MDA)的含量。    结果    与假手术组相比，脑出血模型组大鼠神经功能缺损评分、脑含水量、TNF-α和MDA含量均显著增高（P<0.05）；与脑出血模型组相比，依达拉奉治疗组大鼠神经功能缺损评分、脑含水量、TNF-α和MDA含量显著下降（P<0.05）。    结论    依达拉奉对出血后脑组织具有保护作用，其机制可能与抑制MDA和TNF-α的活性有关。
Abstract:
Objective    To observe the protective effect of edaraven on brain tissue and the therapeutic mechanism in rats following intracerebral hemorrhage(ICH).      Methods    In this series, 96 Sprague-Dawley rats were divided into three groups at random(n=32 in each group)： sham-operative group,ICH model group and edaraven therapy group.  ICH model was induced by infusion of autologous whole blood into the caudate-putamen. Rats in sham-operative group were treated the same as above, but no blood injected. Rats in edaraven therapy group were treated with edaraven (3 mg/kg) by peritoneal injection 30 min before operation and once per 12 hours after operation. These rats were killed at 6, 24, 72, and 168 h after neurologic impairment test with Longa criteria. Brain water content was measured by dry-wet weight method. Malondialdehyde and TNF-alpha in the vicinity of the hematoma were measured by thio-barbituric acid method and ELISA respectively.      Results    Compared with sham operative group, neurologic impairment score, brain water content, malondialdehyde and TNF-alpha in brain tissues were obviously higher in ICH model group (P<0.05). Compared with ICH model group, neurologic impairment score, brain water content, malondialdehyde and TNF-alpha in brain tissues were significantly decreased in edaraven therapy group (P<0.05).      Conclusion    Edaraven has an evident protective effect on injured brain tissue through inhibiting malondialdehyde and TNF-alpha content in the vicinity of the hematoma.

参考文献/References:

崔洁, 曹参祥, 郑静, 等. 脑出血大鼠脑组织MDA、TNF-α含量变化及其依达拉奉干预效应的研究[J]. 第三军医大学学报, 2007, 29(11):1032-1035.

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更新日期/Last Update: 2008-10-23