[1]丁雄,王梦远,刘作金,等.Kupffer细胞在原发性肝细胞癌患者肝脏中的分布及意义[J].第三军医大学学报,2007,29(11):1076-1078.
 DING Xiong,WANG Meng-yuan,LIU Zuo-jin,et al.Distribution of Kupffer cells and its significance in primary hepatocellular carcinoma[J].J Third Mil Med Univ,2007,29(11):1076-1078.
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Kupffer细胞在原发性肝细胞癌患者肝脏中的分布及意义
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《第三军医大学学报》[ISSN:1000-5404/CN:51-1095/R]

卷:
29卷
期数:
2007年第11期
页码:
1076-1078
栏目:
论著
出版日期:
2007-06-15

文章信息/Info

Title:
Distribution of Kupffer cells and its significance in primary hepatocellular carcinoma
作者:
丁雄王梦远刘作金李生伟龚建平
重庆医科大学附属第二医院肝胆外科
Author(s):
DING Xiong WANG Meng-yuan LIU Zuo-jin LI Sheng-wei GONG Jian-ping
Department of Hepatobiliary Surgery, The Second Clinical College, Chongqing Medical University, Chongqing 400010, China
关键词:
肝肿瘤Kupffer细胞
Keywords:
liver tumor Kupffer cells
分类号:
R392-33;R730.23;R735.7
文献标志码:
A
摘要:
目的    观察人原发性肝细胞肝癌患者肝脏Kupffer细胞(KCs)的分布并探讨其意义。      方法    采用免疫组织化学方法,通过CD68定位KCs,运用ImageTool 3.0软件进行细胞计数,了解KCs在正常人肝组织、不同分化程度肝癌组织、癌旁肝组织和远癌肝组织的分布,并在电子显微镜下观察KCs的超微结构改变。      结果    KCs呈卵圆形、星形或纺锤形,在肝癌组织、癌旁肝组织、远癌肝组织和正常肝组织中分布数量分别为(21.6±7.8)、(68.8±9.1)、(62.0±1.9)和(36.2±4.1)(P<0.01),癌组织分化程度越低,其内KCs数量越少(P<0.05)。细胞超微结构观察结果显示,肝癌患者癌旁肝组织肝血窦中KCs数量明显增多,可见中性粒细胞、淋巴细胞和嗜酸性粒细胞浸润,细胞质吞噬体和溶酶体增多。      结论    原发性肝癌患者肝组织内KCs明显激活,但KCs在癌组织内分布数量明显减少,而且癌组织分化程度越低其内KCs数量越少,而在癌旁肝组织和远癌肝组织内较癌组织和正常肝组织显著增多,提示KCs可能通过复杂的途径参与对肝癌的防御作用。
Abstract:
Objective    To observe the distribution  of KCs in liver in human primary hepatocellular carcinoma and investigate the significance.     Methods    KCs were confirmed by immunochemistry of CD68. Image Tool 3.0 software and JEM 2000 electron microscope were employed to define the distribution of KCs in normal human liver tissues, cancer tissues, tissues around and apart from cancer, as well as the number of KCs in different differentiated cancer tissues.     Results    The KCs revealed orbicular-ovate, astroid or fusiform, the number was (21.6±7.8), (68.8±9.1), (62.0±1.9) and (36.2±4.1) respectively in liver cancer tissues, tissues around cancer, tissues apart from cancer, and normal human liver tissues (P<0.01), and the number decreased with the degradation of differentiation degree (P<0.05). Electron microscopy found infiltrations of neutrophils, lymphocytes and eosinophile granulocytes, increased number of KCs in hepatic sinusoid in the tissues around cancer, and increased phagosomes and lysosomes in KCs.     Conclusion    KCs are confirmed greatly activated in liver cancer tissues. The number of KCs declines in liver cancer tissues but increases in tissues around cancer and apart from cancer compared to normal human tissues. This indicates that KCs may participate in the defense to liver cancer by intricate means.

参考文献/References:

丁雄, 王梦远, 刘作金, 等. Kupffer细胞在原发性肝细胞癌患者肝脏中的分布及意义[J]. 第三军医大学学报, 2007, 29(11):1076-1078.

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更新日期/Last Update: 2008-10-23