[1]于月新,李巨.紫草素逆转绒癌细胞JAR/MTX耐药与survivin和Bcl-2表达关系研究[J].陆军军医大学学报(原第三军医大学学报),2007,29(19):1880-1882.
 YU Yue-xin,LI Ju.Relationship between the reverse effect of shikonin on methotrexate-resistant human choriocarcinoma cell line JAR/MTX and expressions of survivin and Bcl-2[J].J Amry Med Univ (J Third Mil Med Univ),2007,29(19):1880-1882.
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紫草素逆转绒癌细胞JAR/MTX耐药与survivin和Bcl-2表达关系研究(/HTML )
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陆军军医大学学报(原第三军医大学学报)[ISSN:1000-5404/CN:51-1095/R]

卷:
29卷
期数:
2007年第19期
页码:
1880-1882
栏目:
论著
出版日期:
2007-10-15

文章信息/Info

Title:
Relationship between the reverse effect of shikonin on methotrexate-resistant human choriocarcinoma cell line JAR/MTX and  expressions of survivin and Bcl-2
作者:
于月新李巨
解放军第202医院妇产科
Author(s):
YU Yue-xin LI Ju
Department of Obstetrics and Gynecology, 202nd Hospital of PLA, Shenyang 110003, China
关键词:
紫草素多药耐药绒毛膜癌逆转
Keywords:
shikonin multi-drug resistance choriocarcinoma reverse
分类号:
R73-36;R730.52;R737.33
文献标志码:
A
摘要:
目的  探讨紫草素逆转绒癌细胞耐药效应在凋亡方面的机制。 方法  体外培养细胞并将其分为4组,各组加药后观察每组细胞24、48、72 h生长情况并计算细胞贴壁率,电化学发光法检测β-HCG值,S-P免疫组织化学及RT-PCR法检测survivin、Bcl-2基因表达。 结果  MTX对细胞JAR/MTX毒性作用、细胞贴壁率、β-HCG下降程度与对照相比差异无统计学意义(P>0.05);紫草素作用细胞后与对照相比差异有统计学意义(P<0.05);二者合用作用于JAR/MTX细胞与对照相比差异显著(P<0.01),survivin基因的mRNA和蛋白表达与对照相比差异显著(P<0.01),Bcl-2基因的mRNA表达与对照相比差异显著(P<0.01),蛋白表达与对照相比差异有统计学意义(P<0.05)。 结论  紫草素可逆转JAR/MTX细胞对MTX耐药是通过下调survivin、Bcl-2基因表达增加细胞凋亡实现的;紫草素有可能成为绒癌化疗的新型增敏剂。
Abstract:
Objective    To explore the mechanism of how shikonin to reverse the multi-drug resistance of human choriocarcinoma cells.     Methods    JAR/MTX cells were divided into 4 groups. The cell growth condition of each group at 24, 48, and 72 h was observed and cell adhesion rate was calculated. β-HCG values at 24 and 48 h after adding drugs were assayed. Cytomorphologic changes were observed by H-E staining. The gene expression of survivin and Bcl-2 was detected by S-P immunocytochemistry and RT-PCR.     Results    There was no significant difference in cell adhesion rate and secretion of β-HCG between methotrexate treatment group and control group (P>0.05), but there was difference between shikonin group and control one (P<0.05).The strong effect was noted after combining use of methotrexate and shikonin as compared with control group (P<0.01). There were significant differences in mRNA and protein expression of survivin and Bcl-2 between shikonin group and control one (P<0.01).     Conclusion    Shikonin can reverse drug-resistance of JAR/MTX to methotrexate by down-regulating the expression of survivin and Bcl-2 and accelerating apoptosis. Shikonin is promising as a synergist of chemotherapy used for choriocarcinoma.

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更新日期/Last Update: 2008-07-15