[1]陈晓玲,杨戎,贾咏存,等.PTEN基因在小鼠胚胎着床过程中作用的实验研究[J].第三军医大学学报,2007,29(21):2064-2066.
 CHEN Xiao-ling,YANG Rong,JIA Yong-cun,et al.PTEN gene expression in mouse endometria increases during embryo implantation[J].J Third Mil Med Univ,2007,29(21):2064-2066.
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PTEN基因在小鼠胚胎着床过程中作用的实验研究(/HTML )
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《第三军医大学学报》[ISSN:1000-5404/CN:51-1095/R]

卷:
29卷
期数:
2007年第21期
页码:
2064-2066
栏目:
论著
出版日期:
2007-11-15

文章信息/Info

Title:
PTEN gene expression in mouse endometria increases during embryo implantation
作者:
陈晓玲杨戎贾咏存刘孝云魏莎莉
重庆医科大学公共卫生学院生殖生物学研究室
Author(s):
CHEN Xiao-ling YANG Rong JIA Yong-cun LIU Xiao-yun WEI Sha-li
Laboratory of Reproductive Biology, Chongqing Medical University, Chongqing 400016, China
关键词:
PTEN子宫内膜植入FQ-PCR子宫角注射
Keywords:
PTEN endometrium implantation real-time fluorescence quantitative PCR intrauterine injection
分类号:
R-33; R321.3; R394.2
文献标志码:
A
摘要:
目的  探讨PTEN基因小鼠胚胎着床过程中的作用。 方法  实时荧光定量聚合酶链反应( realtime fluorescence quantitative PCR ,FQ -PCR)分别检测未孕及孕1、3、4、5、7 d(分别记为d0、d1、d3、d4、d5、d7)小鼠子宫内膜PTEN基因mRNA的表达和子宫角注射反义寡核苷酸观察胚泡着床数。 结果  FQ-PCR测得妊娠的子宫内膜组织PTEN的表达高于未妊娠的子宫内膜组织,且随着妊娠天数的增加呈现逐渐增加的趋势,到妊娠第5天达到最高。子宫角注射PTEN反义寡核苷酸后胚泡着床数明显减少。 结论  PTEN在妊娠早期子宫内膜持续表达,可能参与了胚泡着床。
Abstract:
Objective    To investigate the effect of PTEN gene in the mouse uterus during embryo implantation.    Methods    Real-time fluorescence quantitative PCR (FQ-PCR) was used to detect PTEN mRNA expressed in the endometria of the nonpregnant mice and the late pregnant mice (day 1, day 3, day 4, day 5 and day 7), with 20 mice sacrificed at each fixed day. Out of another 20 3-day pregnant mice, ten received PTEN antisense oligonucleotide at the horn of uterus and ten received normal saline to count the blastocysts at pregnant day 8.    Results    The PTEN mRNA/β-actin mRNA in pregnant mice was higher than that of nonpregnant mice, gradually hoisted as days passed by, and reached the highest at pregnant day 5. The number of blastocysts in the mice that received PTEN antisense oligonucleotide was fewer than that received normal saline.     Conclusion    PTEN persistently expresses in mouse endometria during the early pregnancy and maybe participate in the regulation process of mouse blastodyst implantation.

参考文献/References:

陈晓玲,杨戎,贾咏存,等.  PTEN基因在小鼠胚胎着床过程中作用的实验研究[J]. 第三军医大学学报, 2007, 29(21):2064-2066.

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更新日期/Last Update: 2008-07-02