[1]郑文建,梁平,赵弘智.RhoC-siRNA表达载体对肝癌细胞生长速度及侵袭力的影响[J].陆军军医大学学报(原第三军医大学学报),2007,29(18):1779-1782.
　ZHENG Wen-jian,LIANG Ping,ZHAO Hong-zhi.Role of RhoC-siRNA expression vector on growth velocity and invasiveness of hepatoma carcinoma cells[J].J Amry Med Univ (J Third Mil Med Univ),2007,29(18):1779-1782.
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RhoC-siRNA表达载体对肝癌细胞生长速度及侵袭力的影响(/HTML )
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陆军军医大学学报（原第三军医大学学报）[ISSN:1000-5404/CN:51-1095/R]

卷:
29卷
期数:
2007年第18期
页码:
1779-1782
栏目:
论著
出版日期:
2007-09-30

文章信息/Info

Title:
Role of RhoC-siRNA expression vector on growth velocity and invasiveness of hepatoma carcinoma cells
作者:
郑文建; 梁平; 赵弘智
第三军医大学新桥医院肝胆外科
Author(s):
ZHENG Wen-jian;  LIANG Ping;  ZHAO Hong-zhi
Department of Hepatobiliary Surgery, Xinqiao Hospital, Third Military Medical University, Chongqing 400037, China
关键词:
RhoC; siRNA; 肝癌细胞; 侵袭
Keywords:
RhoC;  siRNA;  hepatoma carcinoma cell;  invasion
分类号:
R394-33；R73-362；R735.7
文献标志码:
A
摘要:
目的  构建RhoC-siRNA表达载体，研究其对肝癌细胞生物学行为的影响。 方法  构建RhoC-siRNA表达载体，以脂质体转染法将之稳定转染入SK-Hep1肝癌细胞株，Western blot法鉴定转染细胞中RhoC蛋白表达的抑制情况，观察转染前后肝癌细胞形态、生长曲线、黏附、运动及体外侵袭能力的改变。 结果  酶切及测序证实RhoC-siRNA真核表达载体构建成功，将之转染入肝癌细胞后，细胞中RhoC蛋白表达水平降低达60％，细胞形态及生长速度无明显改变，但细胞黏附、运动能力及体外侵袭能力均降低。 结论  RhoC-siRNA表达载体对肝癌细胞的形态及生长曲线无显著影响，但可抑制其体外侵袭转移能力,可为肝癌的生物学治疗提供新的方法。
Abstract:
Objective    To construct a RhoC-siRNA expression vector, and study its role on the biological behaviors of hepatoma carcinoma cells.　     Methods    RhoC-siRNA gene was synthesized and cloned into the expression vector pSilencer2.1. The constructed RhoC-siRNA expression vector was stably transfected into hepatoma carcinoma cell line SK-Hep1. The inhibitory effect of RhoC-siRNA on the expression of RhoC in transfected cells was detected by Western blotting. The morphous, growth velocity and the ability of cell adhesion, cell migration, cell invasion before and after transfection was observed.     Results    Enzyme digestion and DNA sequencing confirmed that the RhoC specific siRNA expression vector was constructed successfully. After transfection, RhoC expression was inhibited by 60%, and no marked difference was observed in cellular morphous and growth curve, while the ability of cell adhesion, cell migration, and cell invasion were markedly decreased.     Conclusion    The RhoC-siRNA expression vector can effectively suppress RhoC expression in transfected hepatoma carcinoma cells. Although having no effect on the morphous and growth velocity of hepatoma carcinoma cells, it decreases the potentiality of cell invasion and cell metastasis, which may provide a novel applicable strategy for gene therapy on hepatocellular carcinoma.

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更新日期/Last Update: 2008-07-16