[1]林爽,李力,易萍.低氧对宫颈癌HeLa细胞黏附及侵袭能力的影响[J].第三军医大学学报,2007,29(18):1776-1778.
　LIN Shuang,LI Li,YI Ping.Effect of hypoxia on adhesion and invasion of human cervical cancer cell HeLa[J].J Third Mil Med Univ,2007,29(18):1776-1778.
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低氧对宫颈癌HeLa细胞黏附及侵袭能力的影响(/HTML )
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《第三军医大学学报》[ISSN:1000-5404/CN:51-1095/R]

卷:
29卷
期数:
2007年第18期
页码:
1776-1778
栏目:
论著
出版日期:
2007-09-30

文章信息/Info

Title:
Effect of hypoxia on adhesion and invasion of human cervical cancer cell HeLa
作者:
林爽; 李力; 易萍
第三军医大学大坪医院野战外科研究所妇产科
Author(s):
LIN Shuang;  LI Li;  YI Ping
Department of Obstetrics and Gynecology, Institute of Surgery Research, Daping Hospital, Third Military Medical University, Chongqing 400042, China
关键词:
低氧; 低氧诱导因子（HIF-α）; 肿瘤; 侵袭
Keywords:
hypoxia;  hypoxia inducible factor-1α;  cancer;  invasion
分类号:
R364.4；R73-37；R737.33
文献标志码:
A
摘要:
目的 研究低氧对宫颈癌HeLa细胞的黏附、运动以及侵袭行为的影响。  方法  宫颈癌HeLa细胞放置于常氧、5%O2、3%O2、1%O2培养，逆转录聚合酶链反应（RT-PCR）检测低氧诱导因子HIF-1α、E-钙黏蛋白（E-cadherin）mRNA表达，Transwell小室进行细胞侵袭分析。 结果  常氧、5%O2、3%O2、1%O2条件下HIF-1α mRNA/β-actin光密度比值为（0.423±0.116）、（0.764±0.111）、（1.687±0.126）、（0.215±0.099），随氧分压降低呈现升高后降低的趋势。E-钙黏蛋白/β-actin光密度比值为（1.045±0.171）、（0.719±0.114）、（0.386±0.105）、（0.157±0.063），随氧分压降低而降低。不同氧浓度下细胞侵袭力分别为（112.5±20.7）、（254.3±27.5）、（333.6±45.2）、(124.5±24.6)，随氧分压降低呈现升高后降低的趋势。 结论  适度缺氧可以升高HeLa细胞中HIF-1α的表达，降低肿瘤细胞间黏附力，并增强肿瘤细胞侵袭能力，HIF-1α表达的改变可能是导致肿瘤细胞黏附能力下降、侵袭能力增加的原因之一。
Abstract:
Objective    To investigate the potential effect of hypoxia on adhersion and invasion of human cervical carcinoma cells HeLa.     Methods    Reverse transcription-polymerase chain reaction (RT-PCR) was used to investigate the level of HIF-1α mRNA and E-cadherin mRNA of HeLa cells cultured under normoxia, 5%O2, 3%O2, 1%O2. The invasiveness of HeLa cells was investigated by transwell chamber assay.     Results    Under the condition of normoxia, 5%O2, 3%O2 and 1%O2, the optical density ratio of HIF-1α mRNA/β-actin was (0.423±0.116), (0.764±0.111), (1.687±0.126) and (0.215±0.099), showing that the level of HIF-1α mRNA had a tendency of first inceasing and then decreasing with the decreasing oxygen content. The optical density ratio of E-cadherin/β-actin was (1.045±0.171), (0.719±0.114), (0.386±0.105) and (0.157±0.063), showing a decreasing tendency with the decreasing of oxygen content. The invasiveness of HeLa cells was (112.5±20.7), (254.3±27.5), (333.6±45.2) and (124.5±24.6), showing that the invasiveness of HeLa cells had a tendency of first inceasing and then decreasing with the decreasing oxygen content.     Conclusion    Moderate hypoxia up-regulates the mRNA levels of HIF-1α, and down-regulates the mRNA levels of E-cadherin, and enhances the tumor invasiveness. The possible mechanism is the increasing mRNA levels of HIF-1α.

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更新日期/Last Update: 2008-07-16