[1]左国伟,何焕玲,李星星,等.腺病毒介导BMP-2和BMP-6对人骨肉瘤细胞株MG63和U2OS的作用研究[J].第三军医大学学报,2007,29(18):1768-1771.
 ZUO Guo-wei,HE Huan-ling,LI Xing-xing,et al.Effects of recombinant BMP-2 and BMP-6 adenoviruses on human osteosarcoma cell line MG63 and U2OS[J].J Third Mil Med Univ,2007,29(18):1768-1771.
点击复制

腺病毒介导BMP-2和BMP-6对人骨肉瘤细胞株MG63和U2OS的作用研究(/HTML )
分享到:

《第三军医大学学报》[ISSN:1000-5404/CN:51-1095/R]

卷:
29卷
期数:
2007年第18期
页码:
1768-1771
栏目:
论著
出版日期:
2007-09-30

文章信息/Info

Title:
Effects of recombinant BMP-2 and BMP-6 adenoviruses on human osteosarcoma cell line MG63 and U2OS
作者:
左国伟何焕玲李星星赖天霞王嫣王胜周兰
重庆医科大学生物医学工程系
Author(s):
ZUO Guo-wei HE Huan-ling LI Xing-xing LAI Tian-xia WANG Yan WANG Sheng ZHOU Lan
Department of Biomedical Engineering, Chongqing Medical University, Chongqing 400016, China
关键词:
骨形态发生蛋白细胞增殖细胞凋亡细胞迁移碱性磷酸酶活性
Keywords:
bone morphogenetic protein proliferation apoptosis transmigration phosphatase activity
分类号:
R73-362;R730.23;R738.1
文献标志码:
A
摘要:
目的  研究骨形态发生蛋白(BMP)-2和BMP-6对人骨肉瘤细胞株MG63和U2OS的作用。 方法  分别用重组腺病毒AdBMP-2、AdBMP-6和AdGFP感染骨肉瘤细胞MG63和U2OS,利用台盼蓝拒染法、TUNEL法、吖啶橙/溴乙啶(AO/EB)荧光双染法、Transwell小室和碱性磷酸酶(ALP)活性测定法分别检测细胞增殖、凋亡、迁移以及成骨分化能力的变化。 结果  与感染AdGFP的相应骨肉瘤细胞株(对照组)相比,分别感染AdBMP-2和AdBMP-6的两种骨肉瘤细胞株的细胞存活率随时间延长逐渐降低,凋亡率逐渐增加,细胞穿膜数逐渐减少,ALP活性逐渐增加。 结论  BMP-2和BMP-6可以抑制人骨肉瘤细胞株MG63和U2OS的增殖和迁移,并诱导它们凋亡和向成骨细胞分化。
Abstract:
Objective    To investigate the effects of BMP-2 and BMP-6 on osteosarcoma cell line MG63 and U2OS.     Methods    The two osteosarcoma cell lines were infected with AdBMP-2, AdBMP-6 (as experimental groups), and AdGFP (as control), respectively. Abilities of their proliferation, transmigration, apoptosis and differentiation were detected by trypan blue exclusion test, transwell-room, acridine orange-ethidium bromide fluorescent staining, in situ cell death detection and the test of alkaline phosphatase activity respectively.     Results    In the experimental groups, the cell survival rates were significant decreased (P<0.01); the apoptosis indexes were significantly increased in a time-dependent manner (P<0.01); the cell numbers of transmembrane were decreased (P<0.01); and the activity of alkaline phosphatase was increased gradually (P<0.01).     Conclusion    The human BMP-2 and BMP-6 could inhibit the proliferation and migration, promote apoptosis, and reverse the malignancy of osteosarcoma cell line MG63 and U2OS.

参考文献/References:

左国伟,何焕玲,李星星,等.  腺病毒介导BMP-2和BMP-6对人骨肉瘤细胞株MG63和U2OS的作用研究[J].第三军医大学学报,2007,29(18):1768-1771.

相似文献/References:

[1]周坤,杨辉,周伟,等.药物阻断mGlu3受体通路对脑胶质瘤干细胞增殖与分化的影响[J].第三军医大学学报,2012,34(17):1701.
 Zhou Kun,Yang Hui,Zhou Wei,et al.Effect of drug blockade of type-3 metabotropic glutamate receptor on proliferation and differentiation in glioma stem cells[J].J Third Mil Med Univ,2012,34(18):1701.
[2]林力,邓碧,寿铸,等.硫链丝菌肽对人喉癌Hep-2细胞生长及FoxM1表达的影响[J].第三军医大学学报,2012,34(20):2086.
 Lin Li,Deng Bi,Shou Zhu,et al.Effect of thiostrepton on proliferation and FoxM1 expression in human laryngocarcinoma Hep-2 cell line[J].J Third Mil Med Univ,2012,34(18):2086.
[3]王恒,王勇,江晓霁,等.莪术醇通过JAK3/STAT信号途径抑制Jurkat细胞增殖[J].第三军医大学学报,2013,35(05):408.
 Wang Heng,Wang Yong,Jiang Xiaoji,et al.Proliferation-inhibiting effect of curcumol on human T-cell leukemia cell line Jurkat via blocking JAK3/STAT pathway[J].J Third Mil Med Univ,2013,35(18):408.
[4]邵新宏,于游,张才全.miR-34a靶向调控NOTCH1基因对SW480细胞增殖的影响[J].第三军医大学学报,2012,34(22):2297.
 Shao Xinhong,Yu You,Zhang Caiquan.Inhibitory effects of miR-34a on NOTCH1 gene expression and SW480 cell proliferation[J].J Third Mil Med Univ,2012,34(18):2297.
[5]倪振洪,王滨,丁雯,等.左旋棉酚通过ERK通路诱导Daudi细胞发生自噬及意义[J].第三军医大学学报,2012,34(23):2384.
 Ni Zhenhong,Wang Bin,Ding Wen,et al.(-)-gossypol induces autophagy in Daudi cells through ERK signal pathway[J].J Third Mil Med Univ,2012,34(18):2384.
[6]赵亮,李静,魏强,等.人PGI基因siRNA慢病毒质粒的构建及对白血病细胞增殖的影响[J].第三军医大学学报,2013,35(01):20.
 Zhao Liang,Li Jing,Wei Qiang,et al.Construction of a lentiviral vector expressing human phosphoglucose isomerase gene siRNA and its influence on leukemia cell proliferation[J].J Third Mil Med Univ,2013,35(18):20.
[7]郑英如,李力,刘宝英,等.人可溶性增殖诱导配体促HeLa细胞增殖作用的研究[J].第三军医大学学报,2007,29(20):1951.
 ZHENG Ying-ru,LI Li,LIU Bao-ying,et al.Soluble a proliferation-induced ligand promotes proliferation of HeLa cells[J].J Third Mil Med Univ,2007,29(18):1951.
[8]夏珊,李世荣,刘剑毅,等.大黄素对人增生性瘢痕成纤维细胞的增殖抑制及细胞内游离Ca2+的作用[J].第三军医大学学报,2007,29(20):1982.
 XIA Shan,LI Shi-rong,LIU Jian-yi,et al.Effect of emodin on proliferation and intracellular free calcium of hypertrophic scar fibroblasts in vitro[J].J Third Mil Med Univ,2007,29(18):1982.
[9]冉擘力,何国祥,王耿,等.p27基因对血管平滑肌细胞中连接蛋白43表达的影响[J].第三军医大学学报,2008,30(05):424.
 RAN Bo-li,HE Guo-xiang,WANG Geng,et al.Effect of p27 gene on expression of connexin43 in vascular smooth muscle cells[J].J Third Mil Med Univ,2008,30(18):424.
[10]温磊,孟刚,李懿,等.人骨肉瘤抗失巢凋亡细胞的生物学特性研究[J].第三军医大学学报,2008,30(10):942.
 WEN Lei,MENG Gang,LI Yi,et al.Biological characteristics of anoikis-resistant cells from human osteosarcoma cell line hFOB1.19[J].J Third Mil Med Univ,2008,30(18):942.

更新日期/Last Update: 2008-07-16